Publication: Cinética de hidroxilación y oxidación de compuestos fenólicos por Polifenol Oxidasa de uva Monastrell (Vitis Vinifera)
Authors
Sánchez Ferrer, Álvaro ; Cabanes Cos, Juana ; Bru Martínez, Roque
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Facultad de Biología
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Publisher
Murcia: Universidad de Murcia, Servicio de Publicaciones
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DOI
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info:eu-repo/semantics/article
Description
Abstract
La enzima polifenol oxidasa ha sido purificada a partir de uva Monastrell mostrando actividad tanto en la
oxidación de monofenoles (cresolasa) como en la de o-difenoles (catecolasa). Los parámetros cinéticos, Km y
temperatura óptima, han sido evaluados en ambas actividades. La actividad cresolasa presenta un período de
retardo característico, que puede ser modificado por la temperatura, la concentración de enzima, la presencia
de o-difenoles y la concentración de sustrato.
Monastrell grape polyphenol oxidase was purified, showing both activity towards monophenols and o-diphenols. The kinetic parameters, Km and optimal temperature, were evaluated in both activities. Cresolase activity presents a particular lag period, which may be modified by the temperature, enzyme concentration, the presence of o-diphenols and substrate concentration.
Monastrell grape polyphenol oxidase was purified, showing both activity towards monophenols and o-diphenols. The kinetic parameters, Km and optimal temperature, were evaluated in both activities. Cresolase activity presents a particular lag period, which may be modified by the temperature, enzyme concentration, the presence of o-diphenols and substrate concentration.
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Citation
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