Histology and histopathology Vol.35, nº5 (2020)
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- PublicationOpen AccessEwing's sarcoma of the cervix: a case report and review of literature.(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Zhang, Yangyang; Nong, Weixia; Ren, Yan; Jiang, Jinfang; Zhang, Haijun; Meng, Lian; Li, Qianru; Zhang, Qiaochu; Wang, Xiaomeng; Wang, Yang; Liu, Chunxia; Li, FengEwing’s sarcoma (ES) is a small cell malignant tumor that occurs in the bone of children or adolescents. ES can also occur in extraskeletal organs, such as the pancreas, thyroid, liver, proximal phalanx, and, rarely, cervix. Only 15 published case reports have discussed ES arising in the cervix. We report a 76-year- old woman who had groin mass. ES was diagnosed in accordance with morphological and immunohisto- chemical maps. Fluorescence in situ hybridization and RT-PCR (reverse transcription PCR) revealed ESWR1 gene rearrangement and fusion gene formation (EWS- FLI-1), both of which confirmed the diagnosis of ES. Although the patient underwent surgical resection, the patient died without chemotherapy and radiotherapy. This case is the first one to involve a patient aged over 70 years and the fifth one to show metastasis occurrence
- PublicationOpen AccessThe role of the non-ciliated bronchiolar cell in tolerance to inhaled vanadium of the bronchiolar epithelium(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) López Valdez, Nelly; Guerrero Palomo, Gabriela; Rojas-Lemus, Marcela; Bizarro-Nevares, Patricia; González-Villalva, Adriana; Ustarroz-Cano, Martha; Rivera-Fernández, Norma; Fortoul, Teresa I.The Non-Ciliated Bronchiolar Cell (NCBC) is responsible for the defense and maintenance of the bronchiolar epithelium. Several cellular defense mechanisms have been associated with an increase in the secretion of CC16 and changes in the phenotype of the cell; these mechanisms could be linked to tolerance to the damage due to exposure to inhaled Particulate Matter (PM) of the epithelium. These defense mechanisms have not been sufficiently explored. In this article, we studied the response of the NCBC to inhaled vanadium, an element which adheres to PM. This response was measured by the changes in the phenotype of the NCBC and the secretion of CC16 in a mouse model. Mice were exposed in two phases to different vanadium concentrations; 1.56 mg/m 3 in the first phase and 2.57 mg/m3 in the second phase. Mice were sacrificed on the 2nd, 4th, 5th, 6th and 8th weeks. In the second phase, we observed the following: sloughing of the NCBC, hyperplasia and small inflammatory foci remained without changes and that the expression of CC16 was higher in this phase than in phase I. We also observed a change in the phenotype with a slow decrease in both phases. The increase in the secretion of CC16 and the phenotype reversion could be due to the anti- inflammatory activity of CC16. The changes observed in the second phase could be attributed to the tolerance to inhaled vanadium.
- PublicationOpen AccessDiagnostic utility of CD205 in breast cancer: Simultaneous detection of myoepithelial cells and dendritic cells in breast tissue by CD205(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Ohe, Rintaro; Aung, Naing Ye; Tamura, Yuka; Kabasawa, Takanobu; Utsunomiya, Aya; Tamazawa, Nobuyuki; Kitaoka, Takumi; Meng, Hong-Xue; Shibata, Kenichi; Yamakawa, MitsunoriBackground. CD205 can be used to detect myoepithelial cells (MECs) and dendritic cells (DCs) in breast tissue. However, the usefulness of CD205 immunostaining in the pathological diagnosis of breast tumors is not fully understood. The objective of this study was to re-evaluate CD205 co-expression with other MEC markers, such as p63 and CD10, in nonneoplastic and neoplastic breast tissue and to evaluate its pathological diagnostic utility in these types of breast cancer. Material and methods. Nonneoplastic breast tissue samples with a terminal duct lobular unit and duct were obtained from fibroadenoma and mastopathy patients. Neoplastic breast tissue samples included ductal carcinoma in situ (DCIS) (n=43) and invasive ductal carcinoma (IDC) (n=60), including the tubule-forming type (n=20). These specimens were investigated by CD205, p63, and CD10 immunostaining. Results. In addition to p63 and CD10, CD205 was expressed on MECs in nonneoplastic breast and DCIS tissue samples; CD205 was simultaneously detected on DCs that had infiltrated DCIS and IDC tumor nests. CD205 was expressed on cancer cells themselves in only 7.3% of the breast cancer samples. The number of intratumoral CD205 + DCs in tubular IDC was significantly higher than that in DCIS (P<0.01)
- PublicationOpen AccessPTK7 expression is associated with lymph node metastasis, ALK and EGFR mutations in lung adenocarcinomas(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Jiang, Wei; He, Jing; Lv, Bihong; Xi, Xiaoxiang; He, Guangming; He, JingkangNon-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Lung adenocarcinoma is the main tumor type of NSCLC. Recent advances in the molecular characterization and personalized therapies have improved NSCLC patient prognosis. Previous studies showed that protein tyrosine kinase 7 (PTK7) plays an important role in human cancers. However, the role of PTK7 has not been investigated. PTK7 expression was assessed by immunohistochemistry in 95 patients with lung adenocarcinoma. Correlations of PTK7 expression levels with clinicopathological parameters, EGFR mutation and EML4-ALK fusion were examined. Positive PTK7 expression was detected in 47.4% of lung adeno- carcinoma. PTK7 expression was associated with gender (P=0.024), lymph node metastasis (P<0.001), ALK mutation (P=0.050), and EGFR mutations (P=0.014). No significant association was found between PTK7 expression and age (P=0.831), differentiation (P=0.494), adenocarcinoma subtype (P=0.098) and Ki67 (P=0.473). Our data suggest that PTK7 plays an oncogenic role in lung adenocarcinoma and may be a molecular marker for lymph node metastasis.
- PublicationOpen AccessThe regulatory roles and potential prognosis implications of long non-coding RNAs in gastric cancer(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Wang, Yue; Yang, Fan; Yang, QingAccumulating dysregulated lncRNAs have been demonstrated to execute vital functions in the pathogenesis and progress of gastric cancer (GC) through versatile molecular mechanisms. In this review, we classify the mechanisms of dysregulated lncRNAs in GC into several governing types according to their roles at molecular level. For each regulatory role, we illustrate several instructive examples and introduce significant effects of lncRNAs on cellular biological properties of GC. Besides, we summarize a group of lncRNA- signatures that are potential biomarkers in the prediction of prognosis for GC patients.