Histology and histopathology Vol.35,nº10 (2020)
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- PublicationOpen AccessCORRIGENDUM TO: "Preterm birth and/or low birth weight are associated with periodontal disease and the increased placental immunohistochemical expression of inflammatory markers" Histol Histopathol. 2016 Nov;31(2):231-237. doi: 10.14670/HH-11-671](Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Pozo, Elena; Mesa, Francisco; Ikram, Mohamed H.; Puertas, Alberto; Torrecillas-Martínez, Laura; Ortega-Olle, Inmaculada; Magán-Fernández, Antonio; Rodríguez-Martínez, María Dolores; Miguel, Padial-Molina; Sánchez-Fernández, Elena; Galindo-Moreno, Pablo
- PublicationOpen AccessCombined expression levels of KDM2A and KDM2B correlate with nucleolar size and prognosis in primary breast carcinomas(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Nicola, Igor De; Guerrieri, Ania Naila; Penzo, Marianna; Ceccarelli, Claudio; Leo, Antonio De; Treré, Davide; Montanaro, LorenzoRibosome biogenesis is a fine-tuned cellular process and its deregulation is linked to cancer progression: tumors characterized by an intense ribosome biogenesis often display a more aggressive behavior. Ribosomal RNA (rRNA) synthesis is controlled at several levels, the higher one being the epigenetic regulation of the condensation of chromatin portions containing rRNA genes. KDM2A and KDM2B (Lysine (K)-specific demethylase 2A/B) are histone demethylases modulating the accessibility of ribosomal genes, thereby regulating their transcription. Both enzymes are able to demethylate lysins at relevant sites (e.g. K4, K36) on histone H3. We previously demonstrated that KDM2B is one of the factors regulating ribosome biogenesis in human breast cancer. In this study we aimed to define the combined contribution of KDM2A and KDM2B to breast cancer outcome. KDM2A and KDM2B mRNA levels, nucleolar area as a marker of ribosome biogenesis, and patients' prognosis were retrospectively assessed in a series of primary breast carcinomas. We observed that tumors characterized by reduced levels of both KDM2A and KDM2B displayed a particularly aggressive clinical behavior and increased nucleolar size. Our results suggest that KDM2A and KDM2B may cooperate in regulating ribosome biogenesis thus influencing the biological behavior and clinical outcome of human breast cancers.
- PublicationOpen AccessThe flavonoid 6-hydroxyflavone prevention of cisplatin-induced nephrotoxicity(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Din, Zia Ud; Farooq, Syed Umer; Shahid, Muhammad; Alghamdi, Osama; Al Hamoudi, Nawwaf; Vohra, Fahim; Abduljabbar, TariqIn this study, the flavonoid, 6-hydroxy- flavone was investigated for its renal protective activity in the cisplatin rat model of nephrotoxicity. Male Sprague-Dawley rats weighing 200-250 g were included in the study. 6-Hydroxyflavone was daily administered at 25 and 50 mg/kg (i.p.), while ascorbic acid was used as a positive control and injected (i.p.) at 50 mg/kg for 15 days. The nephrotoxicity was evoked with a single cisplatin injection at 7.5 mg/kg on the tenth day of treatment. The renal function and levels of oxidative stress markers were assessed. Each tissue slide of different groups was observed under a compound microscope attached with a digital camera. Cisplatin significantly decreased the overall body weight with an increase in serum creatinine and urea and production of severe histopathological and oxidative stress in the kidneys. The daily treatment with 6-hydroxyflavone significantly attenuated the cisplatin associated detrimental changes in the body weight, and serum levels of creatinine and urea at both 25 mg/kg (P<0.05) and 50 mg/kg (P<0.01). The 6-hydroxyflavone treatment also preserved the renal histoarchitecture from the toxicological influence of cisplatin as evident from a significant reduction in the severity of histopathological changes in the renal tissues. Moreover, 6- hydroxyflavone also reduced the cisplatin-induced lipid peroxidation and corrected the renal antioxidant status. A similar protective effect was observed with the positive control, ascorbic acid (50 mg/kg). These findings show that the flavonoid 6-hydroxyflavone has potential nephroprotective properties and can be used for the management of chemotherapy associated renal disturbances.
- PublicationOpen AccessHow bad is brazilian ginseng extract for reproductive parameters in mice?(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Dias, F.C.R.; Machado Neves, M.; Lima, G.D.A.; Martins, A.L.P.; Menezes, T.P.; Melo, F.C.S.A.; Gomes, M.L.M.; Cupertino, M.C.; Otoni, W.C.; Matta, S.L.P.Properties attributed to the Panax ginseng are also attributed to the Brazilian ginseng, such as adaptogenic and aphrodisiac effects. There are studies demonstrating that the Brazilian ginseng (BGE) possibly increases the serum levels of testosterone and nitric oxide in mice and rats. The present study aimed to evaluate the effects of its extract on male fertility and sperm quality. Male Swiss mice (n=60) were divided into six groups. The control animals were provided 0.5 mL of water, and 0.5 mL of water containing 7 mg/kg per day (d) sildenafil citrate. Other animals were treated with BGE at 100 mg/kg/d, 200 mg/kg/d, and 400 mg/kg/d by gavage for 42 days. Finally, animals from the last group received 200 mg/kg BGE every 3 days (3- 3d) by gavage for 42 days. The results showed a reduction in the number of resistant spermatids in the testis and damage to daily sperm production, culminating in a reduction in the number of epididymal spermatozoa. Although the sperm quality decreased in all experimental animals, only males treated with BGE 100 mg/kg/d showed pre and post implantation embryo losses. We concluded that BGE alters sperm viability compromising the embryonic development after implantation
- PublicationOpen AccessLncRNA AY343892 inhibits breast cancer development by positively regulating BRCA1-mediated transcription of PTEN(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Li, Yan; Zhang, Miao; Li, Fanreast cancer remains a major challenge despite dramatic advances in cancer research. The long non-coding RNA (lncRNA) has been reported to associate with carcinogenesis and progression of various cancers. In this research, we found that lncRNA AY343892 was significantly down-regulated in breast cancer tissues and cells. Besides, breast cancer patients with high AY343892 level exhibited a favorable prognosis. Functional assays indicated that overexpression of AY343892 significantly inhibited proliferation and promoted apoptosis in breast cancer cells. In terms of mechanism, PTEN and BRCA1 were confirmed to be regulated by AY343892 in breast cancer. Luciferase activity and chromatin immunoprecipitation (ChIP) assays indicated that AY343892 can regulate the promoter of PTEN by binding to BCRA1. Further investigation suggested that knockdown of AY343892 significantly promoted MDA-MB-231 cell proliferation and inhibited MDA-MB-231 cell apoptosis. However, these effects were reversed when PTEN was up- regulated. Moreover, PTEN silence can also countervail the inhibitory effect of overexpressed BCRA1 or AY343892 on the expressions of genes related to proliferation and apoptosis in breast cancer. In conclusion, this study illustrated that AY343892 inhibited breast cancer development by positively regulating BRCA1-mediated transcription of PTEN. This finding contributes to a better understanding in the pathogenesis of breast cancer and provides a theoretical basis for the treatment of breast cancer patients
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