Histology and histopathology Vol. 5, nº 2 (1990)
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- PublicationOpen AccessEffects of experimental diabetes in the noradrenergic and cholinergic nerves of the rat small intestine(Murcia: F. Hernández, 1990) Cuervas-Mons, M.; Morte, L.; Junquera, C.; Ramón y Cajal, S.An histochemical research on cholinergic and noradrenergic fibres of the adventitia layer and of the myenteric plexus of the terminal ileum from rats with streptozotocin-induced diabetes, after 20 weeks of evolution of the illness, was carried out to study changes in the innervation of the gut. The cholinergic nerves, revealed through their acetylcholinesterase activity, did not present alterations, but an evident reduction in number of the noradrenergic nerves and swollen intensely fluorescent varicosities, were observed, both in the perivascular and myenteric plexus of terminal ileum from diabetic animals.
- PublicationOpen AccessModification of swine serum-induced bile duct lesion in BALB/c mice by cyclophosphamide(Murcia: F. Hernández, 1990) Doi, K.; Honjo, K.; Doi, C.; Mitsuoka, T.Effects of cyclophosphamide (CY) on the antibody titer level and incidence and severity of swine serum (SS)-induced bile duct lesion (BDL) were examined. BDL induced by 0.2 m1 of SS per head twice a week for 2 weeks was characterized by hyperplasia of biliary epithelial cells, proliferation of mucous glands, and periductal infiltration of eosinophils with mild fibrosis. CY showed no significant influence on the above-mentioned parameters at the dose levels of 140 and 210 mg/kg. On the other hand, CY lowered the antibody titer level and decreased the seventy of BDL at the dose level of 280 mg/kg, and it suppressed the antibody response and BDL at the dose level of 280 X 2 mg/kg. Thus the antibody titer level and the severity of BDL were closely related each other.
- PublicationOpen AccessInflammatory pseudotumour of the lung. lmmunohistochemical analysis on four new cases(Murcia : F. Hernández, 1990) Barbareschi, M.; Ferrero, S.; Aldovini, D.; Leonardi, E.; Colombetti, V.; Carboni, N.; Mariscotti, C.Inflammatory pseudotumour of the lung (I.P.) is a quite rare benign lesion, variously named by different authors. In the present report four new cases of I.P. are presented and immunohistochemically studied with a panel of antibodies. Microscopically, the most prominent histological features were the presence of interlacing bundles of elongated histiocytic-like cells, plasma cell aggregates and lymphoid follicles. Irnmunohistochemistry showed that plasma cells are polyclonal. The spindle cells were negative for desmin, cytokeratins, lysozyme and S-100 and immunoreactive for alpha-l-antichymotrypsin, vimentin and for smoothmuscle alpha-actin. Actin and desmin, were clearly evident in the vessels' smooth muscle layers, highlighting the angioinvasive behaviour of the lesions. Our data are in keeping with literature suggesting that I.P. is due to a mixed histiocytic-myofibroblastic-reactive proliferation and support the inflammatory nature of IP.
- PublicationOpen AccessDistribution of type IV collagen, laminin, nidogen and fibronectin in the haemodynamically stressed vascular wall(Murcia : F. Hernández, 1990) kittelberger, Reinhold; Davis, Paul F.; Stehbens, William E.Changes in the extracellular matrix of haemodynamically stressed blood vessel walls were studied by immunofluorescence histochemistry in venous-pouch aneurysms fashioned on the site of the common carotid artery of nine sheep. Tissues from the thickened walls of the experimental aneurysms were examined from 11 to 98 months post-operatively for changes in the distribution of the basement membrane components type IV coliagen, laminin, nidogen and fibronectin. In the younger aneurysms, there was an increase of the basement membrane components in the thickened area. Very little basement membrane was detected in older aneurysms. Diffuse staining for fibronectin was noted in aneurysms of al1 ages. Thick deposits of basement membrane material were observed in calcified tissues. The changes in the matrix próteins were similar to alterations occurring dunng the development of atherosclerosis in human vascular tissue.
- PublicationOpen AccessA morphometric study of the secretory granules of the granular duct in the submaxillary gland of the rat following stimulation with noradrenalin and isoproterenol(Murcia : F. Hernández, 1990) Gutiérrez, M.S.; Galera, H.; Bullón, P.; Hevia, A.; Dorado, M. E.In the present work, we carry out a morphometric analysis, at ultrastructural level, of the secretory granules of the granular undulated duct of the submaxillary gland of the rat, under basal conditions (Control Group or I), following stimulation for 10 minutes with 2 mg1100 g weight of Isoproterenol (Group 11), and following stimulation with 2 gamrnas/100 g weight of Noradrenalin for the same time as in the former case. It is seen that in general, Noradrenalin produces the appearance of a greater number of small granules than does Isoproterenol or the control group; and that Isoproterenol induces the presence of larger-sized granules than does Noradrenalin.