Browsing by Subject "Cyclophosphamide"

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    Alveolar cells in cyclophosphamide-induced lung injury. II. Pathogenesis of experimental endogenous lipid pneumonia
    (Murcia : F. Hernández, 1999) Sulkowski, S.; Sulkowska, M.
    An ultrastructural and histological study was made to analyse the structural and cellular features of the pulmonary lesions produced in Wistar rats by intraperitoneal (i.p.) administration of cyclophosphamide (two i.p. doses of 150 mg CP/1 kg bwtl m1 PBS). Rats exposed to cyclophosphamide (CP) developed a condition whose morphological picture corresponded to endogenous lipid pneumonia andlor pulmonary alveolar proteinosis-like changes. Damage to the endothelium and neutrophil accumulation in lung vascular bed were found to be potential initiators of endogenous lipid pneumonia-type changes. The possibility of the evolution of the acute lung injury into endogenous lipid pneumonia-type changes and into alveolar proteinosislike changes was demonstrated. The results of the study supplement the existing theories of pulmonary alveolar proteinosis pathogenesis.
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    Alveolar cells in cyclophosphamideinduced lung injury_ An ultrastructural analysis of type II alveolar epithelial cells in situ
    (F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 1998) Sulkowska, M.; Sulkowski, S.
    Recent studies have brought rich evidence in favour of the significant contribution of the surfactant system-forming structures to morphogenesis of many pulmonary disorders. The aim of this study was to evaluate the effect of intraperitoneal cyclophosphamide administration on changes within this system. The experiments used 40 Wistar rats, of 170g body weight. The animals were divided into two experimental groups. Group I animals were given cyclophosphamide (Endoxan-ASTA) in a single intraperitoneal dose of 150mg/1kg b.w./1ml PBS/. Group II (control) received 1ml PBS. All the animals were sacrificed after 1, 3, 7 and 28 days following intraperitoneal cyclophosphamide or PBS administration. Morphological examinations of pulmonary tissue were based on ultrastructural analysis in the transmission electron microscope. The study revealed that a single intraperitoneal cyclophosphamide injection caused damage to all elements forming the surfactant system, particularly to type II alveolar epithelial cells. Rebuilding processes in pulmonary tissue, coexisting with destructive changes, occurred with a significant contribution of type II alveolar epithelial cells. These cells are likely to take an active part in pulmonary fibrosis processes observed after the action of cyclophosphamide.
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    Modification of swine serum-induced bile duct lesion in BALB/c mice by cyclophosphamide
    (Murcia: F. Hernández, 1990) Doi, K.; Honjo, K.; Doi, C.; Mitsuoka, T.
    Effects of cyclophosphamide (CY) on the antibody titer level and incidence and severity of swine serum (SS)-induced bile duct lesion (BDL) were examined. BDL induced by 0.2 m1 of SS per head twice a week for 2 weeks was characterized by hyperplasia of biliary epithelial cells, proliferation of mucous glands, and periductal infiltration of eosinophils with mild fibrosis. CY showed no significant influence on the above-mentioned parameters at the dose levels of 140 and 210 mg/kg. On the other hand, CY lowered the antibody titer level and decreased the seventy of BDL at the dose level of 280 mg/kg, and it suppressed the antibody response and BDL at the dose level of 280 X 2 mg/kg. Thus the antibody titer level and the severity of BDL were closely related each other.
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    The effects of cyclophosphamide on the gonadotrophic cells of the normal rat
    (Murcia : F. Hernández, 1990) Poblador, María S.; Rojas, Carmen; Raya, Antonio; Casares, José A.; Aparicio, J.; Lancho Alonso, José Luis
    The effects of the administration of cyclophosphamide to male rats at doses of 400 mg/m2 for a period of 5 days and of 200 mg/m2 over two five-day cycles interrupted by a 21-day break reveal differences in the ultrastructural morphology of gonadotrophic cells between treated animals and normal controls. The ultrastructural data obtained coincide with the results obtained from the measurement of serum FSH and LH levels, indicating hypofunction. A significant fall was also recorded in blood testosterone levels. The two types of treatment did not give rise to morphological or functional differences.
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    The effects of cyclophosphamide on the prolactin cells of the normal rat
    (Murcia : F. Hernández, 1989) Poblador, María S.; Rojas, Carmen; Raya, Antonio; Quiralte, Joaquín; Casares, José A.; Lancho Alonso, José Luis
    Cyclophosphamide administered at doses of 400 mg/m2/5 days with sacrifice two days later, and 200 mg/m2/5 days with a 21-day break and a further five days of treatment, with sacrifice two days later, provokes similar effects. Functional activity is less marked in untreated and control animals than in treated ones, as the hormone data shows. But the ultrastructure of PRL cells in treated animals indicates the existence of clear hormone synthesis activity, evident in the fusion and clustering of granules at differing stages of maturity, etc.
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    Ultrastructural alterations of the cortical epithelial cells of the rat thymus after cyclophosphamide treatment
    (Murcia : F. Hernández, 1997) Yoon, S.; Yoo, Y.H.; Kim, B.S.; Kim, J.J.
    A single dose of cyclophosphamide (CY) was administered to Sprague-Dawley rats to investigate the effects of CY on thymic cortical epithelial cells (TCE) at the ultrastructural level. The most striking finding among the alterations in the TCE after CY treatment was a cytoplasmic vacuolization with an increased amount of granular and membranous content. The granular content appeared not only as dense bodies but also as loosely aggregated forms or finely dispersed grandes. The membranous structures appeared in various forms including vesicular, tubular, vacuolar and irregular membranous structures and myelin figures. Some of the membranous structures contained granular material. Severa1 vacuoles were closely associated with the endoplasmic reticulum (ER). The morphological alterations of the ER were also remarkable. The Golgi apparatus, mitochondria and vesicles increased in number. The cytoplasm became densely granulated due to an increased number of ribosomes and an increased amount of granular material. The tonofilaments lost their original array and increased in amount. The cell surface exhibited many cytoplasmic processes like microvilli. It seems that the above features result not only from some damage by CY, but are also signs of a hyperfunctiona1 state of the TCE, probably due to their important functions in repopulation and maturation of the cortical thymocytes during recovery after CY-induced acute thymic involution, including the secretion of some humoral factors.