Publication: Cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro
Authors
Morcillo, Patricia ; Romero, Diego ; Meseguer, José ; Cuesta Peñafiel, Alberto ; Esteban Abad, María de los Ángeles
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Publisher
Springer
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DOI
http://doi.org/10.1007/s11356-016-6445-3
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info:eu-repo/semantics/article
Description
©2016 Springer. This document is the Published version of a Published Work that appeared in final form in Environmental Science and Pollution Research. To access the final edited and published work see https://doi.org/10.1007/s11356-016-6445-3
Abstract
The in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. In this study, erythrocytes from marine gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed for 24 h to Cd, Hg, Pb and As and the resulting cytotoxicity was evaluated. Exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxicity followed by MeHg, Cd, As and Pb. Moreover, fish erythrocytes incubated with each one of the metals exhibited alteration in gene expression profile of metallothionein, superoxide dismutase, catalase, peroxiredoxin, glutathione reductase, heat shock proteins 70 and 90, Bcl2-associated X protein and calpain1 indicating cellular protection, stress and apoptosis death as well as oxidative stress. This study points to the benefits for evaluating the toxicological mechanisms of marine pollution using fish erythrocytes in vitro.
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Citation
Environmental Science and Pollution Research, Vol. 23, N. 12, 2016, pp. 12312-12322.
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