Browsing by Subject "Erythrocytes"

Now showing 1 - 6 of 6
  • Thumbnail Image
    Publication
    Open Access
    Cadmium- and lead-induced apoptosis in mallard erythrocytes (Anas platyrhynchos)
    (Elsevier, 2009-01) Romero García, Diego; Hernández-García, A.; Tagliati, C. A.; Martínez-López, E.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    Cadmium, lead and cadmium–lead (1:10) induced apoptosis were studied using mallard blood cells. The allowable range in concentrations were: 0.01–0.5, 0.1–5.0, and 0.01:0.10–0.50:5.00 mM, for cadmium, lead and cadmium–lead, respectively. The lowest EC50 achieved was for cadmium (0.2270.04 mM). Two doses from each treatment group were chosen to study apoptosis and the presence of metals in cells. The percentage of apoptotic cells increased as the concentration of metals increased. The percentage of cells with intracellular metals was high for both exposure levels and the quantity of intracellular metal was greater for exposure to high concentrations. Morphological alterations for all types of exposure were related to the diverse range of effects that these metals have on membranes. We suggest that the decrease in the number of erythrocytes observed in specimens suffering from lead and cadmium poisoning is related to the induction of apoptosis.
  • Thumbnail Image
    Publication
    Restricted
    Cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro
    (Springer, 2016-03-15) Morcillo, Patricia; Romero, Diego; Meseguer, José; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Ciencias Sociosanitarias
    The in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. In this study, erythrocytes from marine gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed for 24 h to Cd, Hg, Pb and As and the resulting cytotoxicity was evaluated. Exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxicity followed by MeHg, Cd, As and Pb. Moreover, fish erythrocytes incubated with each one of the metals exhibited alteration in gene expression profile of metallothionein, superoxide dismutase, catalase, peroxiredoxin, glutathione reductase, heat shock proteins 70 and 90, Bcl2-associated X protein and calpain1 indicating cellular protection, stress and apoptosis death as well as oxidative stress. This study points to the benefits for evaluating the toxicological mechanisms of marine pollution using fish erythrocytes in vitro.
  • Repository logo
    Publication
    Open Access
    Effects of cantharidin on fish erythrocytes, tumour cell lines, and marine pathogenic bacteria
    Campos-Sánchez, José Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
    Cantharidin, a toxic monoterpene secreted by blister beetles, has long been used in traditional Chinese and modern medicine for its unique properties. However, despite its widespread use, its effects on fish have not been studied in depth. The aim of this study was to evaluate the potential therapeutic applications of cantharidin in fish by examining its antioxidant, hemoagglutinating, hemolytic and cytotoxic activities at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 μg mL-1) in three different cell lines. In addition, the study explored the bactericidal and bacteriostatic properties of cantharidin against various fish pathogenic bacteria. The results revealed that there were no significant differences in antioxidant, hemagglutinating or hemolytic activities between the different concentrations of cantharidin tested. However, the study found that cantharidin exhibited dose- and time-dependent cytotoxicity in sea bream (Sparus aurata) erythrocytes and in SAF-1, PLHC-1 and Hela cell lines, resulting in morphological changes indicative of apoptosis. Interestingly, the highest dose of cantharidin tested demonstrated potent bactericidal activity against four marine fish opportunistic bacteria, including Vibrio harveyi, V. anguillarum, Photobacterium damselae and Tenacibaculum maritimum, but no statistically significant changes in bacteriostatic activity were observed against any of the bacteria tested. Overall, these results provide valuable information on the potential therapeutic applications of cantharidin in fish aquaculture. Further research is needed to fully understand the mechanisms of action and to explore possible preventive uses of cantharidin in fish.
  • Repository logo
    Publication
    Open Access
    Erythrocyte phagocytosis in gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax).
    (2024-11-04) Campos Sánchez, Jose Carlos; Meseguer, José; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
  • Repository logo
    Publication
    Open Access
    In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens
    Campos-Sánchez, José Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
    Carrageenin is a high molecular weight sulphated mucopolysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenin. The results showed that λ-carrageenin has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 hours of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenin. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 hours of incubation compared with control cells, and the highest doses of λ-carrageenin caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC1 cells incubated with the highest doses of λ-carrageenin for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 or HeLa cell lines after incubation with λ-carrageenin. λ-carrageenin showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, V. anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenin has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.
  • Thumbnail Image
    Publication
    Restricted
    In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of Common buzzard (Buteo buteo)
    (Elsevier, 2013-11-25) Hernández-García, A.; Romero, D.; María-Mojica, P.; Martínez-López, E.; García Fernández, Antonio Juan; Gómez Ramírez, Pilar; Ciencias Sociosanitarias
    Cadmium and lead are persistent and ubiquitous metals that can cause several deleterious effects in living beings. Apoptosis and necrosis are two types of cell death that can be found after in vivo and in vitro exposure to these metals. In this study, isolated red blood cells from living captive Common buzzard (Buteo buteo) were exposed in vitro to different concentrations of lead, cadmium, and the mixture lead–cadmium in a proportion of 1:10 (similar to that found in previous field studies). Data obtained from dose–response curves were used to evaluate the interactive effects of metal mixtures on cell viability. In general, except for the exposure to NOEC, additivity was the most frequently observed response. As described in human, after in vitro exposure, lead was highly accumulated in buzzard erythrocytes, while cadmium accumulation was scarce. Finally, the type of cell death (apoptosis or necrosis) induced by the exposure to different concentrations of these heavy metals and their mixtures was evaluated in the red blood cells. Apoptosis was found to be the main type of cell death observed after cadmium and/or lead exposure. However, this exposure caused an increase in lysis or necrosis, especially if red blood cells were exposed to high doses.