Cuesta Peñafiel, Alberto

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Cuesta Peñafiel, Alberto

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Cuesta Peñafiel, Alberto

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Universidad de Murcia. Departamento de Biología Celular e Histología

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Open Access
Esferoides y esferas líquidas. Cultivos celulares en 3D para mimetizar el ambiente de las células en el organismo
(Universidad de Murcia, 2015) Meseguer, José; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Mulero Méndez, Victoriano Francisco; Sepulcre Cortés, María Pilar
Embargo
Molecular oxidative stress markers in olive ridley turtles (Lepidochelys olivacea) and their relation to metal concentrations in wild populations
(Elsevier, 2017-10-23) Cortés-Gómez, Adriana A.; Morcillo, Patricia; Espinosa, Cristóbal; Girondot, Marc; Romero, Diego; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Ciencias Sociosanitarias
Due to their longevity and extensive migration areas, marine turtles are able to accumulate diverse contaminants over many years and as a consequence they represent an interesting bioindicator species for marine ecosystem pollution. Metals provoke toxicological effects in many aquatic animal species, but marine turtles have been under-investigated in this area. Thus, we have determined the presence of certain inorganic elements (As, Cd, Cu, Ni, Pb, Se and Zn) in olive ridley turtles (Lepidochelys olivacea) and related them to metallothionein (MT), superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) transcription and/or enzymatic activities. Gene expression of sod, cat and gr was found to be higher in blood than liver or kidney but most of the significant relationships were found in liver, not only for gene expression but also for enzyme activities. This must be related to the role the liver has as the first filter organ. Several positive relationships ofsod, cat and gr gene expression in the different tissues were found in this population, as well as very high Cd concentrations. This could mean that these turtles are adapting to the metals-production of ROS and damage through a high transcription of these antioxidants. Multiple positive relationships with GR seem to be part of its compensatory effect due to the decrease of SOD production against the high and chronic exposure to certain xenobiotics. CAT, on the other hand, seems not to be used much, and glutathione detoxification of H2O2 may be more important in this species. Finally, despite the very high Cd concentrations found in this population, no significant relationship was found in any tissue with metallothionein gene expression. These results, along with very high Cd concentrations and a negative relationship with Cu, lead us to consider some kind of disruption in mt gene expression in these turtles.
Open Access
Data of the work entitled: Ability of short-chain fatty acids to reduce inflammation and attract leucocytes to the inflamed skin of gilthead seabream (Sparus aurata L.)
Albaladejo-Riad, Nora; El qendouci, Mouna; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Biología Celular e Histología
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Cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro
(Springer, 2016-03-15) Morcillo, Patricia; Romero, Diego; Meseguer, José; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Ciencias Sociosanitarias
The in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. In this study, erythrocytes from marine gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed for 24 h to Cd, Hg, Pb and As and the resulting cytotoxicity was evaluated. Exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxicity followed by MeHg, Cd, As and Pb. Moreover, fish erythrocytes incubated with each one of the metals exhibited alteration in gene expression profile of metallothionein, superoxide dismutase, catalase, peroxiredoxin, glutathione reductase, heat shock proteins 70 and 90, Bcl2-associated X protein and calpain1 indicating cellular protection, stress and apoptosis death as well as oxidative stress. This study points to the benefits for evaluating the toxicological mechanisms of marine pollution using fish erythrocytes in vitro.
Open Access
Gene expression raw data of the work entitlled: "evolution of a gilthead sea bream (Sparus aurata L.) caudal fin skin explant"
Albaladejo-Riad, Nora; García-Álvarez, Miguel Ángel; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Marín Parra, Claudia; Biología Celular e Histología
Open Access
Terminal carbohydrates abundance, immune related enzymes, bactericidal activity and physico-chemical parameters of the Senegalese sole (Solea senegalensis, Kaup) skin mucus
(Elsevier , 2016-11-09) Collado-González, Mar; Guardiola Abellán, Francisco Antonio; Cuartero, María; Díaz Baños, F. Guillermo; Cuesta Peñafiel, Alberto; Moriñigo, Miguel Angel; Esteban Abad, María de los Ángeles; Biología Celular e Histología
Recently, interest in mucosal surfaces, more specifically fish skin and its secreted mucus, has greatly increased among immunologists. The abundance of terminal carbohydrates, several enzymes (proteases, lysozyme, peroxidase, alkaline phosphatase, esterases and ceruloplasmin), bactericidal activity against fish pathogenic and non-pathogenic bacteria and several physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolarity, density and viscosity) in the skin mucus of Senegalese sole (Solea senegalensis, Kaup) have been evaluated. Present results evidence the abundance of N-acetylneuraminic acid, mannose, glucose and N-acetyl-galactosamine in skin mucus. The levels of lysozyme, proteases, esterases and alkaline phosphatase were very similar (from 20 to 30 Units mg−1 protein). However, 93 Units mg−1 protein were detected of ceruloplasmin and only 4′88 Units mg−1 protein of peroxidase. Skin mucus of S. senegalensis showed high bactericidal activity against the tested pathogen bacteria but weak activity against non-pathogenic bacteria. Finally, a clear relationship between mucus density and temperature was detected, while viscosity showed a direct shear- and temperature-dependent behaviour. These results could be useful for better understanding the role of the skin mucus as a key component of the innate immune system, as well as, for elucidating possible relationships between biological and physico-chemical parameters and disease susceptibility.
Restricted
Data set of Novel hepcidin genes in Gilthead Seabream: implications for immune response and iron metabolism
(2025-04-24) García Navarro, Laura; Serna Duque, Jhon; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Biología Celular e Histología
Results of Synteny and phylogeny analysis of hamp2.0 and hamp2.15 in the class Actinopterygii, Bactericidal activity of HAMP2α or HAMP2Ω, Cytotoxicity activity of HAMP2α or HAMP2Ω. And a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems).
Open Access
Effect of Light–Dark Cycle on Skin Mucosal Immune Activities of Gilthead Seabream (Sparus aurata) and European Sea Bass (Dicentrarchus labrax)
(MDPI, 2020) Ceballos-Francisco, Diana; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Biología Celular e Histología
Changes in di erent immune activities in the skin mucus of gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens exposed to a constant light–dark photoperiod (12 h L:12 h D) were studied. Samples were collected at 08:00 (light on), 14:00, 20:00 (light o ), 02:00, and again at 08:00 to determine immunoglobulin M (IgM) levels, several enzymes related to the immune system, and bactericidal activity. IgM levels were higher during the day in seabream and reached a minimum value at 20:00, but it was hardly a ected in sea bass. No significant variations were recorded in the levels of protease and antiprotease. Peroxidase reached its maximum level in seabream at 02:00, the same time that it reached its minimum level in sea bass. Lysozyme showed little variation in seabream, but it was significantly lower at 14:00 than during the rest of the cycle in sea bass. Finally, di erent interspecific variations on bactericidal activity against Vibrio harveyi were recorded. The findings demonstrate that the immune parameters present in skin mucus of these important fish species are a ected by the light–dark cycle and that there are substantial interspecies di erences.
Open Access
Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass
(Elsevier, 2024-03) Garcia Alvarez, M. A.; Chaves Pozo, E.; Cuesta Peñafiel, Alberto; Biología Celular e Histología
Cell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes, as target cells. Results showed low and unaltered innate cytotoxic activity through the infection time. However, adaptive CMC against RGNNV and SJNNV/RGNNV-infected target cells increased from 7 to 30 days post-infection, peaking at 15 days, demonstrating the specificity of the cytotoxic activity and suggesting the involvement of CTLs. At transcriptomic level, we observed up-regulation of genes related to T cell activation, perforin/granzyme and Fas/FasL effector pathways as well as apoptotic cell death. Further studies are necessary to understand the adaptive role of European sea bass CTLs in the elimination of NNV-infected cells.
Open Access
Bioinformatic and gene expression analysis of the somatostatin/cortistatin gene family in the gilthead seabream (Sparus aurata).
Campos Sánchez, Jose Carlos; Serna‑Duque, Jhon A.; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología