Publication: Biotechnological production of antioxidant pigments betalains and evaluation of their functional capacity in the in vivo model Caenorhabditis elegans
Authors
Guerrero Rubio, Mª Alejandra
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Escuela Internacional de Doctorado
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Gandía Herrero, Fernando
Publisher
Universidad de Murcia
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info:eu-repo/semantics/doctoralThesis
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Abstract
La metodología biotecnológica desarrollada en esta Tesis Doctoral ha permitido estudiar las propiedades promotoras de la salud de diecisiete betalaínas individuales empleando el modelo animal Caenorhabditis elegans. Los resultados mostraron que los gusanos alimentados con betalaínas puras experimentaron un aumento en su tiempo de vida útil. El mecanismo molecular subyacente a este efecto se analizó usando cepas mutantes y realizando ensayos de microarrays a partir de extracción de ARN. Los resultados mostraron que las betalaínas modulan la expresión de daf-16 o skn-1, genes ortólogos a FOXO y Nrf2 humanos, respectivamente. En ambas especies, estos genes están involucrados en vías relacionadas con la longevidad y la resistencia al estrés oxidativo y conducen a la sobreexpresión de HSPs, proteínas involucradas en la resistencia al cáncer y al Alzheimer. Estos resultados pueden abrir nuevas líneas de investigación en la búsqueda de tratamientos efectivos basados en estas moléculas de origen vegetal
The biotechnological methodology developed in this Thesis has allowed to study the health-promoting properties of seventeen individual betalains by employing the animal model Caenorhabditis elegans. Results showed that worms fed with pure betalains experienced an increase in their lifespan. The molecular mechanism underlying this effect was analyzed by using mutant strains and by performing microarray assays from the extraction of RNA from worms fed with betalains. The results showed that betalains modulate the expression of daf-16 or skn-1, orthologous genes to human FOXO and Nrf2, respectively. In both species, these genes are involved in pathways related to longevity and oxidative stress resistance and lead to the overexpression of hsp genes that encode HSP proteins involved in resistance to cancer and Alzheimer's. Therefore, the results obtained can open new lines of research in the search for effective treatments based on these molecules of plant origin.
The biotechnological methodology developed in this Thesis has allowed to study the health-promoting properties of seventeen individual betalains by employing the animal model Caenorhabditis elegans. Results showed that worms fed with pure betalains experienced an increase in their lifespan. The molecular mechanism underlying this effect was analyzed by using mutant strains and by performing microarray assays from the extraction of RNA from worms fed with betalains. The results showed that betalains modulate the expression of daf-16 or skn-1, orthologous genes to human FOXO and Nrf2, respectively. In both species, these genes are involved in pathways related to longevity and oxidative stress resistance and lead to the overexpression of hsp genes that encode HSP proteins involved in resistance to cancer and Alzheimer's. Therefore, the results obtained can open new lines of research in the search for effective treatments based on these molecules of plant origin.
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