Oñate Sánchez, Ricardo Elías

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Oñate Sánchez, Ricardo Elías

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Oñate Sánchez, Ricardo Elías

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Universidad de Murcia. Departamento de Dermatología, Estomatología,Radiología y Medicina Física

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Open Access
Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells
(Willey, 2016-09-26) Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Ortolani-Seltenerich, P.S.; Lozano, Adrián; Forner, Leopoldo; Llena, Carmen; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física
Aim To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs). Methodology human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound-healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One-way analysis of variance (ANOVA) followed by a Bonferroni post-test was performed (P < 0.05). Results At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer (BR) and Nano-ceramic Sealer (NCS), but not Endoseal MTA (ES). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation (P < 0.05). Similar results were obtained in a cell death assay. In addition, hPDLSCs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR. Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs. Conclusions BR and NCS were associated with better cytocompatibility than ES. Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate-based endodontic sealers for clinical application.
Open Access
Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth
(Willey, 2017-02-07) Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Ortolani-Seltenerich, P.S.; Álvarez-Muro, T.; Lozano, A.; Forner, L.; Llena, C.; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física
Aims To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint-Maur-des-Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs). Methodology SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound-healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post-test (a = 0.05). Results Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P < 0.01) and was also significantly higher than using MTA Angelus from 48 h of incubation (P < 0.01). However, Theracal LC and IRM were associated with low rates of cell viability (P < 0.001). Similar results were obtained in an apoptosis assay. In addition, SHEDs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. SEM studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and MTA Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7 days of culture (P < 0.01). Conclusions Biodentine exhibited better cytocompatibility and bioactivity than MTA Angelus, Theracal LC and IRM.
Open Access
La férula gingival como método modificado de aplicación tópica de esteroides en el penfigoide cicatrizal oral
(Elsevier, 1992-06) Bermejo Fenoll, Ambrosio; Sánchez Pérez, Arturo Joaquín; Oñate Sánchez, Ricardo Elías; López Jornet, María Pía; González Sequeros, Ofelia; Dermatología, Estomatología, Radiología y Medicina Física
Se describe un nuevo método en el tratamiento de la membrana mucosa penfigoide oral. Consiste en una técnica modificada de aplicación local de esteroides, en cura oclusiva. El fármaco es colocado en las áreas de lesión, fundamentalmente encías y paladar, mediante férulas de resina rígida, montadas en articulador semiajustable. Presentamos 4 casos clínicos, y se obtuvo mejoría en todos ellos. No hubo efectos adversos locales o sistémicos.
Open Access
Silk fibroin and graphene oxide composites promote human periodontal ligament stem cell spontaneous differentiation into osteo/cementoblast-like cells
(Mary Ann Liebert, Inc, 2016-11-15) Sánchez Vera, María del Mar; Aznar-Cervantes, Salvador; Jover, Eva; García Bernal, David; Oñate Sánchez, Ricardo Elías; Hernández Romero, Diana; Moraleda Jiménez, José María; Collado-González, Mar; Rodríguez Lozano, Francisco Javier; Cenís, José L.; Dermatología, Estomatología, Radiología y Medicina Física
Graphene represents one of the most interesting additions to the tissue engineering toolbox. Novel graphenebased composites are required to improve the beneficial graphene properties in terms of tridimensional polymeric structure, conferring a higher mechanical strength and favoring the differentiation of human mesenchymal stem cells. Here, we have demonstrated in a wide range of composite combinations, the successful use of graphene and silk-fibroin constructs for future bioengineering applications in the field of clinical regenerative dentistry using human periodontal ligament stem cells. Our results provide exciting new data for the development of suitable scaffolds that allow good cell engrafting, preservation of cell viability and proliferation, promotion of spontaneous osteoblastic differentiation, and importantly, stimulation of a higher cementum physiological synthesis than using other different available biomaterials.
Open Access
Citotoxicity of Guttaflow bioseal, Guttaflow2, MTA Fillapex, and AH Plus on human periodontal ligament stem cells.
(Elsevier Science, 2017-03-23) Collado-González, Mar; Tomás Catalá, Christopher Joseph; Oñate Sánchez, Ricardo Elías; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física
Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment. Methods: Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer. To assess cell orphology and attachment to the different sealers, the hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Chemical composition of the sealers was determined by energy-dispersive x-ray, and eluates were analyzed by inductively coupled plasma mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was evident after 24 hours in the presence of GuttaFlow Bioseal and GuttaFlow 2 but not in the case of AH Plus or MTA Fillapex. At 168 hours, GuttaFlow Bioseal and GuttaFlow 2 exhibited high and moderate cell viability, respectively, whereas AH Plus and MTA Fillapex revealed low rates of cell cell viability (P < .001). Finally, scanning electron microscopy studies revealed a high degree of proliferation, cell spreading, and attachment, especially when using GuttaFlow Bioseal disks. Conclusions: GuttaFlow Bioseal and GuttaFlow2 showed lower cytotoxicity than MTA Fillapex and AH plus. Further in vitro and in vivo investigations are required to confirm the suitability of GuttaFlow Bioseal for clinical application.
Open Access
Biological effects of acid-eroded MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells
(2019-01-25) Rodríguez Lozano, Francisco Javier; Oñate Sánchez, Ricardo Elías; Collado-González, Mar; López-García, Sergio; García Bernal, David; Tomás Catalá, Christopher Joseph; Moraleda Jiménez, José María; Lozano, Adrián; Forner, Leopoldo; Dermatología, Estomatología, Radiología y Medicina Física
Objective The aim of this study was to analyze the biological effects of MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells (hPDLSCs) after exposure to acidic and neutral environments. Materials and methods Discs of each material (n = 30) were exposed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.2) for 7 days, and biological testing was carried out in vitro on hPDLSCs. Cell viability and apoptosis assays were performed using eluates of each root-end filling material. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the rootend filling materials was determined by energy-dispersive x-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by ANOVA and Tukey test (p < 0.05). Results Under an acidic environment, both materials displayed similar ion release abilities, with the increased release of Si and Ca ions. Substantial changes in microstructure were observed for both materials after exposure to acidic pH. In addition, material exposure to an acidic environment showed a similar degree of cell adherence, and, surprisingly, MTA Repair HP exhibited higher cell viability rates at pH 5.2 than ProRoot MTA. Conclusions Exposure to an acidic environment promoted Si and Ca ion release from ProRoot MTA and MTA Repair HP. Moreover, we observed optimal biological properties of ProRoot MTA and MTA Repair HP in terms of cell viability, cell death, and cell attachment in both environments. Clinical relevance These results may suggest that MTA Repair HP and ProRoot exhibited optimal biological properties in terms of cell viability, cell death and cell attachment in acidic environment, being considered as materials for root-end filling and perforations.
Open Access
Dental treatments under general anesthesia on children with special health care needs enrolled in the spanish dental care program
(MDPI, 2021-01-06) Pecci Lloret, Miguel Ramón; Guerrero Gironés, Julia; López-González, Belén; Rodríguez Lozano, Francisco Javier; Oñate-Cabrerizo, Daniel; Oñate Sánchez, Ricardo Elías; Pecci-Lloret, Miguel R.; Dermatología, Estomatología, Radiología y Medicina Física
Abstract: The purpose is to analyze the medical characteristics of children with special health care needs (CSHCN) recommended for dental treatment under general anesthesia (GA), postoperative complications, and dental treatment outcomes under the regulation of the Spanish Dental Care Program (PADI). 111 clinical records were selected. The study population was divided into three age groups. The quantitative data was specified as the mean ± SD. For the qualitative variables, the Chi- Square test was used. One-way ANOVA and Bonferroni tests were used to examine the effect of the “age group” and the number of treatment procedures. A total of 1473 treatment procedures were performed, of which 110 (7.5%) were cleanings, 898 (61%) were restorative procedures, 332 (21.7%) were extractions, 22 (1.6%) were endodontic treatments, 62 (4.2%) were pulpotomies, and 59 (4%) were stainless steel crowns. Regarding the mean number of incisor root canal treatments (RCT), age group 3 received a significantly higher mean number of incisor RCTs than age group 1 (p = 0.02). Age group 1 received a higher average of pulpotomies and stainless-steel crowns (p = 0.00) compared to groups 2 and 3. GA is a safe procedure for the dental treatment of CSHCN, with minimal postoperative complications, which should be included among dental public programs.
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Clinical management of the homozygous α-thalassemia with unusual mandibular manifestation of hematopoiesis
(Elsevier, 2017-02-03) Ruiz Roca, Juan Antonio; Oñate Sánchez, Ricardo Elías; Urrutia Rodríguez, Idoia; Martínez-Izquierdo, A.; Mengual-Pujante, D.; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
Alpha (α)-thalassemias are the most common genetic disorder of hemoglobin (Hb) synthesis, affecting up to 5% of the world's population. These congenital hemolytic anemias induce extramedullary hematopoiesis, including the liver, spleen, sinuses, and the diploic spaces of the skull. Oral health problems in patients with thalassemias are mostly related to a varied degree of facial deformities, malocclusions, and/or dental arch dimensions. We present a case with a 49-year-old man, diagnosed with homozygous α thalassemia that came to the Faculty of Dentistry at the University of Murcia for a dental treatment. It was observed that the patient had an unusual mandibular manifestation of hematopoiesis.
Open Access
Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment
(2019-03-03) Rodríguez Lozano, Francisco Javier; Collado-González, Mar; López-García, Sergio; García Bernal, David; Moraleda Jiménez, José María; Lozano, Adrián; Forner, Leopoldo; Murcia, Laura; Oñate Sánchez, Ricardo Elías; Biología Celular e Histología
Aim To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (a < 0.05). Results The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.
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Dental extractions management in Bernard-Soulier syndrome
(Lippincott, Williams & Wilkins, 2015-09) Ruiz Roca, Juan Antonio; Oñate Sánchez, Ricardo Elías; Cabrerizo Merino, María del Carmen; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina