Hernández Ruiz, Josefa

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Hernández Ruiz, Josefa

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Hernández Ruiz, Josefa

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Universidad de Murcia. Departamento de Biología Vegetal

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Open Access
Validation of an automated assay for the measurement of cupric reducing antioxidant capacity in serum of dogs.
(BioMed Central, 2016) Peres Rubio, Camila; Tvarijonaviciute, Asta; Martínez-Subiela, Silvia; Cerón, J.J.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
Background: The objective of the present study was to optimize and validate an automated method to assess the total antioxidant capacity (TAC) in serum of dogs using the cupric reducing antioxidant capacity (CUPRAC) methodology (TACc) with bathocuproinedisulfonic acid disodium salt as chelating agent, evaluating also possible variations due to the use of two different automated analyzers. The method is based on the reduction of Cu2+ into Cu1+ by the action of the non-enzymatic antioxidants that are present in the sample. Results: Imprecision was low in both apparatus utilized, and the results were linear across serial Trolox and canine serum samples dilutions. Lipids did not interfere with the assay; however, hemolysis increased the TACc concentrations. When TACc concentrations were determined in ten healthy (control) dogs and in twelve dogs with inflammatory bowel disease (IBD), dogs with IBD had lower TACc concentrations when compared with the healthy dogs. Conclusions: The method validated in this paper is precise, simple, and fast and can be easily adapted to automated analyzers.
Open Access
Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: An update
(BioMed Central, 2016-08-15) Peres Rubio, Camila; Martínez-Subiela, Silvia; Tvarijonaviciute, Asta; Cerón, J.J.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
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Serum biomarkers of oxidative stress in dogs with idiopathic inflammatory bowel disease
(Elsevier, 2017-03) Peres Rubio, Camila; Martínez-Subiela, S.; Tvarijonaviciute, Asta; Cerón, J.J.; Allenspach, K.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
The objective of this work was to study and compare a panel of various serum biomarkers evaluating both the antioxidant response and oxidative damage in dogs with idiopathic inflammatory bowel disease (IBD). Eighteen dogs with IBD and 20 healthy dogs were enrolled in the study. Trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of the plasma (FRAP), total thiol concentrations, and paraoxonase 1 (PON1) activity were evaluated in serum to determine antioxidant response. To evaluate oxidative status, ferrous oxidation-xylenol orange (FOX), thiobarbituric acid reactive substances (TBARS) and reactive oxygen species production (ROS) concentrations in serum were determined. Mean concentrations of all antioxidant biomarkers analyzed, with exception of FRAP, were significantly lower (P < 0.0001) in the sera of dogs with IBD than in healthy dogs. The oxidant markers studied were significantly higher (P < 0.0001) in sera of dogs with IBD than in healthy dogs. These findings support the hypothesis that oxidative stress could play an important role in the pathogenesis of canine IBD.
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Mecanismo de la inactivación de peroxidasas vegetales por hidroperóxidos: implicación en procesos fisiológicos y biotecnológicos / Josefa Hernández Ruiz ; directores Manuel Acosta Echevarría y Marino Bañón Arnao.
(Murcia : Universidad de Murcia, Departamento de Biología Vegetal,, 1999) Hernández Ruiz, Josefa
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Changes in serum biomarkers of oxidative stress after treatment for canine leishmaniosis in sick dogs
(Elsevier, 2016-12) Peres Rubio, Camila; Martínez-Subiela, Silvia; Tvarijonaviciute, Asta; Pardo-Marin, Luis; Segarra, S.; Cerón, J.J.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
Canine leishmaniosis (CanL) is a zoonotic disease being endemic in several parts of the world. In this studywe investigated the behavior of a panel of biomarkers of oxidative stress in 12 sick dogs naturally infectedby CanL before and at days 30 and 180 of a successful therapy with a standard treatment. The assays totaloxidant status (TOS), trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma(FRAP), cupric reducing antioxidant capacity (CUPRAC), serum thiol and paraoxonase 1 (PON1) wereincluded in the panel. In addition, correlations between biomarkers of oxidative stress and inflammation(C-reactive protein (CRP) and ferritin) and urinary protein:creatinine ratio (UPC) were calculated. SerumCUPRAC, thiol and PON1 significantly increased after treatment and were negatively correlated with CRP,ferritin and UPC. This study demonstrates that biomarkers of oxidative stress, not previously studied inleishmaniosis such as CUPRAC and thiol, can change after a successful treatment for CanL showing apotential for use in monitoring the treatment of this disease