Histology and histopathology Vol. 2, nº 4 (1987)

Ir a Estadísticas

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http://hdl.handle.net/10201/177571

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Ultrastructure of the tubular nephron of Testudo graeca (Chelonia). A comparison between hibernating and non-hibernating animals
(Murcia : F. Hernández, 1987) Zuasti, A.; Ferrer, C.; Ballesta Germán, José; Pastor García, Luis Miguel
The tubular nephron of hibernating and nonhibernating specimens of Testudo graeca (Chelonia) was studied by means of conventional light and electron microscopy and histochemistry. The tubular nephron was composed of proximal, intermediate, distal and collecting tubules in both hibernating and nonhibernating animals. The cells of the proximal tubule showed long microvilli, cytoplasmic vacuoles, a developed endoplasmic reticulum and abundant mitochondria. Fat droplets were also observed. The intermediate segment was lined by ciliated and nonciliated cells. The lining cells of the distal tubule presented few microvilli, abundant dense mitochondria and clear vesicles of mucous appearance in the terminal portion. Collecting ducts are composed of mucous and non-mucous cells. Mucous cells presented strong reaction to the histochemical techniques detecting sialoand sulpho-mucins. During hibernation, a progressive vacuolar degeneration of the endoplasmic reticulum was observed in al1 the segments of tubular nephron, which may be caused by a massive intake of extracellular water into the cell.
Open Access
Morphometric study of hepatic ultrastructure in alcoholic hepatitis
(Murcia : F. Hernández, 1987) Chen, T.S.; Murphy, D.P.; Marquet, G.; Chedid, A.; Mendenhall, C.L.; Rabin, L.; Veterans Administration Cooperative Study Group on Alcoholic Hepatitis
We undertook a morphometric analysis of hepatocellular organelles in an attempt to correlate their changes with the clinical stages of patients.with alcoholic hepatitis. Although hepatic ultrastructural alterations did not correlate with disease severity, we found significant differences between patient and control groups in the measured parameters of non-organelle cytoplasm, mitochondria, SER, RER, glycogen, and lipid.
Open Access
Thermotherapy for Harding-Passey melanoma, light and electron microscopic study
(Murcia : F. Hernández, 1987) Vicente, Vicente; Gómez, M.; Gómez, S.; Calderon, F.; Canteras Jordana, Manuel; Cremades, A.
The changes of implanted Harding-Passey melanoma in C57Blí6J mice following treatment with wholebody hyperthermia were studied. The treated tumours showed a progressive growth delay, cellular and architectural irregularities as well as cell injury characteristics. The presence of distended and irregular blood vessels, the peripheral localization of the melanosomes and the melanosome complexes were constant.
Open Access
Interstitial collagen in alcoholic human liver
(Murcia : F. Hernández, 1987) Stenbäck, F.; Wasenius, Veli-Matti; Sotaniemi, Eero A.
The occurrence and intensity of staining for specific antibodies against the aminoterminal propeptide of type 111 procollagen (PIIIP), which is indicative of the synthesis and the degradation of that collagen type, was studied in sections from normal and alcoholic livers and compared with serum PIIIP levels, serum antipyrine clearance, fibronectin distribution and morphology as revealed by conventional stains and electronmicroscopy. Positive staining for PIIIP and fibronectin was observed in the perisinusoidal space of the normal liver and in portal tracts. In alcohol-induced fatty liver positive staining increased around the central veins, in alcoholic hepatitis increased staining reaction was seen to a limited extent in areas of cell injury. Extensive reticulin and PIIIP-positive areas were found in the periportal interstitium of the cirrhotic livers and in large fibrotic areas extending into the surrounding parenchyma in cases of active disease. The results show a distinct relationship between collagen type 111 metabolism, morphologically detectable hepatic injury and liver cell function tests, with tissue deposition occurring later in the disease process than biochemically detectable serum collagen levels and signs of altered liver cell function.
Open Access
Immunohistochemical change of actin in exprerimental myocardial ischemia. Its usefulness to detect very early myocardial damages
(Murcia : F. Hernández, 1987) Shozo Nishida; Shingo Hiruma; Shigeo Hashimoto
Pathomorphological diagnosis of acute myocardial infarction has many problems in human autopsy materials less than 4 to 6 hours after clinical onset and in rats 2 to 3 hours after experimental coronary occlusion. Since immunohistochemical reaction deuends - ~ on the antigei determinant site of the material, chañges in the reaction may reflect alterations at the molecular level in myocardial fibers. With this consideration in mind, the effectiveness of diagnosing infarction at the earliest (possible) stage, and the changes of actin filaments were investigated through experiments, using immunohistochemical methods involving anti-actin antibodies produced from chicken gizzards in our laboratory. The left coronary arteries of rats were ligated to produce ischemia. Dehydrogenases were shown to be still present by triphenyltetrazolium chloride (TTC) reaction, but the anti-actin antibody reaction had disappeared in areas corresponding to the ischemic sites. However, on electron microscopic examination of these sites, actin fibers were clearly revealed. In the case of ischemia lasting for more than 6 hours, the anti-actin antibody reaction had disappeared, corresponding to the disappearance of the TTC reaction. At this stage, myocardial actin fibers were revealed by electron microscopic examination. These results indicate that ischemia induces some type of biochemical degeneration at the molecular level of myocardial actin, most likely the change of actin polymerization. Moreover, they show that the anti-actin antibody technique is capable of detecting such very early degenerative and ischemic changes proving itself to be better suited for determining the range and degree of early infarction.

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