Histology and histopathology Vol. 5, nº 1 (1990)

Ir a Estadísticas

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    Morphological studies of cytotoxic lesions in reversible endotoxic shock
    (Murcia : F. Hernández, 1990) García, R.; Díaz-Laviada, l.; Bosch, M.A.; Abarca, S.; Portolés, M.T.; Risco, C.; Ainaga, M.J.; Pagani, R.; Municio, A.M.; Escalona, J.
    Reversible endotoxic shock was induced in adult rats by intravenous injection of E. coli Olll:B4 lipopolysaccharide (LPS) and the progression of metabolic and morphological alterations was evaluated. Serum samples and biopsies from adrenal gland, liver and lung were studied at different times after LPS injection. Histological changes in these tissues were observed after endotoxin administration, coinciding with both the acute-phase and the recovery-phase of shock (24-72h after LPS injection). Signs of tissue regeneration can be correlated with the regression of some serum parameters to their normal values. Al1 these results indicate that in this experimental model of endotoxic shock, a reversible status was established, which will allow further studies of the endotoxic pathophysiological mechanisms in vivo, avoiding the complexity of the non-reversible process.
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    Subcutaneous fat necrosis of newborn children
    (Murcia : F. Hernández, 1990) Salas Valién, J.S.; Ribas Ariño, M.T.; Egido Romo, M.; Palau Benavides, M.T.
    We report a case of subcutaneous fat necrosis of a newborn child which appeared 9 days after birth and was cured without any complications. We propose the reclassification of the etiological factors accompanying this lesion by classifying them in a plurietiological syndrome with some basic or essential factors and other causative factors.
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    Frog stomach enteric plexuses in culture, isolation, morphological characterization and bioelectrical recordings
    (Murcia : F. Hernández, 1990) Azanza, M.J.; Peg, M.T.; Junquera, C.; Aisa, J.; Martínez Ciriano, C.; Blasco, J.
    We have succeeded in the isolation, culture and morphological characterization of Rana ridibunda stomach enteric plexuses. We have furthermore obtained intra and extracellular bioelectric recordings from the explants in culture. The cultare medium used (Eagle MEM), the collagenase digestion and the general culture conditions followed are similar to those applied to mammal enteric plexus explant cultures. The most striking difference is that the solutions were diluted to 70% in order to maintain the osmolar conditions required by the amphibian cells. Acetylcholinesterase, osmium tetroxide-zinc iodide- and para-formaldehydeinduced fluorescence methods reveal similar morphological images from the perivascular fibre plexuses. The different cell types observed by phase contrast light microscopy from the myenteric explants in culture have been identified by comparison with those revealed by the acetylcholinesterase method. The prevailing neurons show piramidal somas; other neurons are bipolar with oval somas and a third type shows oval somas tightly aligned, following sinusoidal courses. The intra and extracellular bioelectric recordings from the explants in culture show that the culture conditions we have applied preserve the electrophysiological properties of the neuronal membranes. These preliminary recordings will allow us to undertake the synaptic characterization of the gastrointestinal neurotransmitters in frogs.
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    Postnatal development of the dog pineal gland. Light microscopy
    (Murcia : F. Hernández, 1990) Calvo, J.L.; Boya, J.; García-Mauriño, A.; López Carbonell, A.
    The light microscopical morphology of the dog pineal gland from the first postnatal day to maturity is described. In the first postnatal week, the pineal parenchyma shows immature cells and many mitotic figures. In this week, pigmented cells are obsemed for the first time, both in the pineal gland and in extrapineal nodules. Throughout the second week, the pineal parenchyma shows a cordonal pattern that disappears progressively in the following stages. From the 20-30th day onward, it is feasible to discern the cell types characteristic for the adult pineal gland. In the adult animals, the length of the pineal gland axes almost quadruplies that of the pineal gland in neonatal stages. The light microscopical features of the adult dog pineal gland are also described.
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    Cytoarchitecture of the dorsal lateral geniculate nucleus in the rabbit
    (Murcia : F. Hernández, 1990) Carmona Martos, R.; Abadía Molina, F.; Calvente Iglesias, R.; Abadía Fenoll, F.
    The cytoarchitecture and morphometry of the neurons in the a, sector of the dorsal lateral geniculate nucleus of the rabbit have been studied. The preparation techniques used were those of Golgi-Adams and Klüver-Barrera. Our method was to subdivide the a, sector into three zones (superior, media1 and inferior) and then to cut each zone along the horizontal, transverse and saggital planes in order to obtain precise measurements and descriptions of the neurons whatever their orientation. Differences exist in both the size and distribution of neurons in the inferior zone of the a, sector compared to the other two.