Publication: Frog stomach enteric plexuses in culture, isolation, morphological characterization and bioelectrical recordings
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Date
1990
Authors
Azanza, M.J. ; Peg, M.T. ; Junquera, C. ; Aisa, J. ; MartÃnez Ciriano, C. ; Blasco, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We have succeeded in the isolation, culture
and morphological characterization of Rana ridibunda
stomach enteric plexuses. We have furthermore
obtained intra and extracellular bioelectric recordings
from the explants in culture. The cultare medium used
(Eagle MEM), the collagenase digestion and the general
culture conditions followed are similar to those applied
to mammal enteric plexus explant cultures. The most
striking difference is that the solutions were diluted
to 70% in order to maintain the osmolar conditions
required by the amphibian cells. Acetylcholinesterase,
osmium tetroxide-zinc iodide- and para-formaldehydeinduced
fluorescence methods reveal similar morphological
images from the perivascular fibre plexuses. The
different cell types observed by phase contrast light
microscopy from the myenteric explants in culture have
been identified by comparison with those revealed by the
acetylcholinesterase method. The prevailing neurons
show piramidal somas; other neurons are bipolar with
oval somas and a third type shows oval somas tightly
aligned, following sinusoidal courses. The intra and
extracellular bioelectric recordings from the explants in
culture show that the culture conditions we have applied
preserve the electrophysiological properties of the
neuronal membranes. These preliminary recordings will
allow us to undertake the synaptic characterization of the
gastrointestinal neurotransmitters in frogs.
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