Histology and histopathology Vol. 7, nº 4 (1992)
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- PublicationOpen AccessDermal cylindroma. Expression of intermediate filaments, epithelial and neuroectodermal antigens(Murcia : F. Hernández, 1992) Wollina, Uwe; Rülke, D.; Schaarschmidt, H.We report on irnmunohistochemical staining pattems in so-called apocrine tumors of skin with special emphasis on the dermal cylindroma. The results were compared with apocrine tubular adenoma, syringocystadenoma papilliferum and the normal eccrine sweat gland. A relationship of dermal cylindroma to the apocrine gland is suggested by expression of lysozyme and alphal-antichymotrypsin. The tumor shares keratin, epithelial membrane antigen (EMA) and EGF-receptor expresssion with eccrine and apocrine glands. The presence of intermingled cells with a coexpression of keratin and vimentin argues for a partial myoepithelialike differentiation. Neuroectodermal antigens are rnissing. Therefore, dermal cylindroma is classified as an adnexal tumor of skin with a variable rate of cells of apocrine secretory, myoepithelial and undifferentiated phenotypes
- PublicationOpen AccessSex-specific response of the vasopressin-reacting neurons of the paraventricular nucleus of the rat hypothalamus following chronic administration of met-enkephalin(Murcia : F. Hernández, 1992) Sánchez, F.; González, R.; Carretero, J.; Rubio, M.; Riesco, J. M.; Blanco, E.; Juanes, J.A.; Vázquez, R.Using the peroxidase-antiperoxidase immunocytochernical technique, a morphometric study of the magnocellular neurons of the Paraventricular nucleus of the rat hypothalamus, reactive to specific anti-vasopressin rabbit serum, was made. Following systemic and chronic administration of met-enkephalin the number of immunoreactive neurons was higher, especially in females. Additionally, in the females, it was possible to observe an increase in the immunoreactivity and the presence of well-stained fibres. These findings suggest, especially in females, a blockage in the release of vasopressin, facilitating its immunocytochemical visualization.
- PublicationOpen AccessIron-binding proteins in human colorectal adenomas and carcinomas: an immunocytochemical investigation(Murcia : F. Hernández, 1992) Tuccari, G.; Rizzo, A.; Crisafulli, C.; Barresi, G.By immunocytochemistry, the presence of major iron-binding proteins (lactoferrin, transferrin and ferritin) was investigated in tubular adenomas (12 cases), villous adenomas (7 cases), carcinomas of the large bowel and rectum (39 cases) and lymph nodes involved in carcinomas (8 cases); 5 samples of colonic inflarnrnatory pseudopolyps were aiso studied. Dysplastic areas of tubular and villous adenomas as well as adenocarcinomas and colloid carcinomas showed a variable cytoplasmic immunoreactivity for al1 antisera, although no staining was noted in some cases; tubular adenomas without dysplasia and colonic inflarnmatory pseudopolyps were always unstained. Metastatic elements present in lymph nodes maintained the irnmunohistochemicai staining for iron-binding proteins. An autoctone production of lactoferrin, transferrin and ferritin by tumour cells may be hypothesized in relation to the increased requirement of iron for the tumover of rapidly dividing cells.
- PublicationOpen AccessExpression of intermediate filaments and other special markers by testicular germ cell tumors. With reference to embryogenesis(Murcia : F. Hernández, 1992) Hanai, J.; Lin, M.; Wada, A.; Ishiguro, S.; Miki, T.; Sakaguchi, H.; Kanda, H.Distribution of intermediate filament proteins (IFs) and severa1 special markers was studied in 39 testicular germ cell tumors and 8 embryos and foetuses. The similarity and difference between development of germ cell tumor and embryogenesis were immunohistochemically investigated. Seminoma and embryonal carcinoma, as tumoral counterparts of undifferentiated germ cells, were characterized by little IF expression. This study revealed that the maturing and differentiating process in germ cell tumor is different from normal embryonal development and the tumor cells showed leaping maturing steps in tumorigenesis. Immunostaining for IFs helped to discover the further differentiation occurring in embryonal carcinoma and to demonstrate heterogeneous elements in non-seminoma germ cell tumors, which sometimes might not be apparent by light microscopical observation of H&E staining section. According to the findings, two patterns in mixed germ cell tumors are suggested; Le., combined and diffuse types. The mechanism of tumorigenesis of the two types is supposed to be different. Clinically, the prognosis of most patients with testicular germ cell tumor is fairly good because of the improved chemotherapies that are dependent on histological diagnosis.
- PublicationOpen AccessExpression of neuronal and glial markers in so-called oligodendrogilial tumors induced by transplacental administration of ethyl-nitrosourea in the rat(Murcia : F. Hernández, 1992) Vaquero, J.; Coca, S.; Moreno M.; Oya, S.; Arias, A.; Zurita, M.; Morales, C.A series of 18 tumors with histological features of oligodendrogliomas, induced in the rat by means of transplacental ethyl-nitrosurea administration were studied for immunohistochemical demonstration of neuronal (synaptophysin and neurofilament protein) and glial (gliofibrillar acidic protein and vimentin) markers. Most of the tumors showed cells with strong positivity to synaptophysin and to a lesser degree, to neurofilament protein, suggesting the neuronal character of these neoplasms. In 10 tumors, cells with strong positivity to vimentin were found, and in three cases, tumoral cells expressed gliofibrillar acidic protein. The observation that ENU-induced oligodendroglial tumors express neuronal and, to a minor degree, glial markers, suggests their interpretation as prirnitive neuroectodermal tumors with clear neuronal differentiation.