Histology and histopathology Vol.32, nº1 (2017)
Ir a Estadísticas
Permanent URI for this collection
Browse
Recent Submissions
- PublicationOpen AccessThe role of dendritic cells in immune regulation of nasal polyps(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Liang, Zhuoping; Yang, Ting; Xu, Wei; Huang, Ying; Jiang, Liang; Yin, Zedeng; Qin, GangNasal polyps (NPs) are caused by a variety of immune cells and inflammatory cells. However, as the most potent antigen-presenting cells in the immune system, the role of dendritic cells (DCs) in NPs is still unclear. In the present research, we studied the role of DCs in immune regulation of NPs. Thirty patients with NPs, who served as the experimental group, received systemic and local glucocorticoids for 4-7 d, and specimens were collected prior to hormone treatment and during surgery. Normal middle turbinate mucosa tissues from 18 patients who underwent nasal septum surgery were collected as controls. The expression levels of CD83, tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and eosinophils (EOS) in NP tissues before and after glucocorticoid therapy and in control middle turbinate mucosa tissues were studied. After glucocorticoid therapy, the expression levels of CD83, TNF-α, IL-4 and EOS decreased significantly. In addition, the expression of IL-4 was lower than that of TNF-α, reversing the Th2 cytokine-dominant condition. CD83 and EOS showed a positive correlation. DCs participated in the development and progression of NPs and could promote the generation of Th2 cytokines. After interference by glucocorticoid therapy, DCs could inhibit the expression of Th2 cytokines and induce secretion of Th1 cytokines. DCs and EOS thus might both play roles in promoting the development and progression of NPs, but the underlying mechanism requires further study.
- PublicationOpen AccessRenovascular hypertensive decrease immunoreactivity of cells containing chromogranin A and pancreastatin in the pancreas of rats(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Piotrowska, Żaneta; Janiuk, Izabela; Lewandowska, Alicja; Kasacka, IrenaHypertension significantly increases the risk of hyperglycemia in patients. It is known that chromogranin (CgA) and pancreastatin (PST) are involved in regulation of blood pressure and endocrine function of the pancreas. However, still little is known about the physiology of these hormones’ secretion in hypertension. The objective of the study was to examine the effects of hypertension on pancreatic islet cells containing CgA and PST in rats. The studies were carried out on the pancreas of rats. After 6 week period of the renal artery clipping procedure, eight 2K1C rats developed stable hypertension. Cells containing CgA and PST were detected using immunohistochemical method. The hypertension significantly decreased the number of pancreatic endocrine cells immunoreactive to CgA and PST antisera. The differences between the hypertensive and normotensive rats concerned not only the number of endocrine cells but also intensity of reactions. In conclusion, the research results indicate that hypertension causes the diminished biosynthesis of CgA and PST in the pancreas of rats and suggests the participation of those peptides in pancreatic disorders occurring in a state of elevated blood pressure.
- PublicationOpen AccessThe suppressive effects of miR-1180-5p on the proliferation and tumorigenicity of bladder cancer cells(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Ge, Qiangqiang; Wang, Chenghe; Chen, Zhong; Li, Fan; Hu, Jia; Ye, ZhangqunIn our previous research, we have reported that a candidate microRNA (miR-1180-5p) has the capacity to induce overexpression of tumor suppressor gene p21 and inhibit the growth of human bladder cancer (BCa) cell lines in vitro. However, the exact mechanism as to how miR-1180-5p suppresses BCa cell proliferation remains unknown, and the inhibitory effect of miR-1180-5p in vivo also need to be investigated. In the present study, we found that the expression level of miR-1180-5p was lower in BCa cells than in normal human urothelial cells. Furthermore, we found that overexpression of p21, activated by miR-1180-5p, interfered with cell cycle progress by inhibiting the cell cycle related proteins (CDK4, CDK6, Cyclin D1 and Cyclin A2), and thereby suppressed BCa cell proliferation. In addition, miR-1180-5p also suppressed the tumor growth in vivo significantly. Taken together, our study provides evidence that up-regulation of p21 is mainly responsible for the suppressive effect of miR1180-5p on BCa cells and miR-1180-5p can significantly inhibit tumorigenicity in vivo.
- PublicationOpen AccessProtective effect of 2-deoxy-D-glucose on the brain tissue in rat cerebral ischemia-reperfusion models by inhibiting caspase-apoptotic pathway(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Min, He-ming; Wang, Yan; Ren, Da Yong; Cheng, Xue; Li, Jian; Jiang, Xing qian; Min, Lian qiu; Bao, Cui fenWe observed the effect of 2-deoxy-D-glucose (2-DG) on the brain tissue in rat cerebral ischemiareperfusion (I/R) and explored its mechanism. After observing the effect of 2-DG on endoplasmic reticulum stress (ERS), rats were randomly divided into shamoperation group, I/R group and I/R+2-DG group (each group with 60 rats). I/R models were prepared by middle cerebral artery occlusion. In I/R+2-DG group, each rat was given intraperitoneal 2-DG of 100 mg/kg once a day for 7 days before brain ischemia. According to different time points (3 h, 6 h, 12 h, 24 h and 48 h) after I/R, each group was divided into 5 subgroups (each subgroup with 12 rats). Nerve cell apoptosis, and the expressions of mRNA and protein of glucose regulated protein 78 (GRP78), cleaved-caspase-9 and cleaved-caspase-3 were determined with TUNEL, Western blotting and RT-PCR, respectively, in rat cerebral hippocampal CA1 area at each time point. TUNEL-positive cells were significantly less in I/R+2-DG group than in I/R group at each time point (all P<0.01). In I/R and I/R+2-DG groups, the expressions of mRNA and protein of GRP78 reached the maximum 12 h after I/R, and cleavedcaspase-9 and cleaved-caspase-3 reached the maximum 24 h after I/R. Compared with sham-operation group, the expressions of mRNA and protein of GRP78, cleavedcaspase-9 and cleaved-caspase-3 were all significantly increased (all P<0.01) in I/R and I/R+2-DG groups. However, the expressions of mRNA and protein of GRP78 were significantly higher in I/R+2-DG group than in I/R group (all P<0.05), but the expressions of mRNA and protein of cleaved-caspase-9 and cleavedcaspase-3 were all significantly lower in I/R+2-DG group than in I/R group (all P<0.05). We conclude that 2-DG has a neuroprotective effect on the brain tissue in rat cerebral ischemia-reperfusion models. The mechanism may be that 2-DG starts ERS followed by up-regulation of mRNA and protein of GRP78 and down-regulation of mRNA and protein of cleavedcaspase-9 and cleaved-caspase-3, which blocks the apoptotic pathway.
- PublicationOpen AccessGenistein attenuates monocrotaline-induced pulmonary arterial hypertension in rats by activating PI3K/Akt/eNOS signaling(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Zheng, Zeqi; Yu, Songping; Zhang, Wan; Peng, Yongchao; Pu, Mingyu; Kang, Ting; Zeng, Junyi; Yu, Yuefei; Li, GuorongIntroduction: Phytoestrogen genistein may be useful to treat pulmonary arterial hypertension (PAH). However, its mechanism is still not clear. The aim of the present study was to confirm the therapeutic effects of phytoestrogen genistein on PAH in monocrotalineinduced rat model and to explore its mechanism. Materials and Methods: Sprague-Dawley male rats were randomly divided into 4 groups: control group (n=8), PAH group (n=8), genistein treament group with three different doses (n=8 in each dose group) and group of PI3K inhibitor LY294002. The rat model of PAH was induced by monocrotaline (MCT). The situation of survival of rats was observed. Pathological studies of lung and heart tissues were performed. Western-blot detection of P-Akt and P-eNOS expression levels in lung tissue was carried out. Nitrate reductase analysis was used to measure nitric oxide (NO) in lung tissue. Results: Genistein treatment resulted in significant improvement in the speed of tricuspid regurgitation, diameter of pulmonary artery, mean pulmonary artery pressure and right ventricular hypertrophy index. Genistein treatment also resulted in significant improvement in the stenosis of pulmonary artery, proliferation of smooth muscle, right ventricular hypertrophy and myocardial hypertrophy. These therapeutic effects were more obvious with increasing dose of genistein. After genistein treatment, amelioration