Histology and histopathology Vol. 3, nº 4 (1988)

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http://hdl.handle.net/10201/177572

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Electron microscopic pathological patterns of alveolar septum in acute dextran-induced and alloxan-induced pulmonary edema in dogs
(Murcia : F. Hernández, 1988) Koji Nakahara; Michio Arakawa; Kenjiro Kambara; Hiroshi Oda; Senri Hirakawa; Shizuko Shoumura; Hideo Isono
We studied the incidence of electron microscopic pathological patterns of the alveolar septum observed 30 min after induction of pulrnonary edema by dextran-70 infusion (6 dogs, dextran group) and by alloxan injection (6 dogs, alloxan group). For comparable amounts of extravascular lung water in both dextran and alloxan groups, which were twice as much as control group (6 dogs), we characterized the pathological changes. The incidence of the electron microscopic pathological patterns that appeared in dextran group compared with that in control group was significantly high in terms of the widening of the interstitial space. dispersion and disarray of collagen fibrils, and erythrocytes in the interstitial space. The incidence in alloxan group compared with that in control group was significantly high in terms of the swelling of epithelial cells and endothelial cells as well as the widening of the interstitial space, and dispersion and disarray of collagen fibrils. We conclude that dextran causes interstitial changes exclusively and alloxan causes cellular changes primarily coupled with secondary interstitial changes in acute pulmonary edema.
Open Access
Electron microscopic observations on the formation of elastic fibres in cultures of aortic medial cells and adventitial cells
(Murcia : F. Hernández, 1988) Masumi Akita; Katsuji Kaneko; Merker, H. J.
The formation of elastic fibres was observed in the cultured cells derived from the tunica media and the tunica adventitia of mouse aorta. Bundles of myofilaments with dense bodies were abundantly observed in the cytoplasm of the cultured media1 cells, and numerous bundles of microfibrillar components were present in the intercellular spaces. Fine granules of approximately 50 nm in diameter were observed in the bundles of microfibrillar components. lt was supposed that these fine granules of elastin fused with each other and formed elastic aggregates and then formed large elastic clumps. Numerous bundles of microfibrillar components were also present in the intercellular spaces of the cultured adventitial cells. Elastic aggregates were scarcely observed in the bundles of microfibrillar components. However, large elastic clumps as observed in the media1 cell culture could not be found in the adventitial cell culture. It is suggested that the formation of large elastic clumps might be related to the sheet structures or lamellae of elastic fibres in the tunica media.
Open Access
The lectin binding pattern of normal and pathologically altered synovial tissue
(Murcia : F. Hernández, 1988) Zschabitz, A.; Stofft, E.
Light-microscopical lectin-binding studies were carried out in healthy and pathologically altered synovial tissue (osteoarthrosis, rheumatoid arthritis (RA)). Seven lectins were studied: Con A, DBA, PNA, RCA, SBA, UEA-1, and WGA. Con A and WGA mark al1 lining cells and the majority of subintimal synovial cells. RCA and SBA stain only a portion of lining cells, regardless of the basic pathology. The lectin PNA reacts only with RA and arthrotic material, and is thus suitable for the diagnosis of inflammatory changes in synovial tissue. UEA-1 is a consistent marker for capillary endothelium and large vessels.
Open Access
Abnormal development of the notochord and perinotochordal sheath in duplicitas posterior, patch and tail-short mice
(Murcia : F. Hernández, 1988) Center, Elizabeth M.; Marcus, Normala M.; Wilson, D.B.
Interest in developmental interactions involving the notochord and perinotochordal sheath led to a comparative investigation of these structures in three mouse mutants. Alcian blue or periodic acid-Schiff staining of 9 1/2-13 days' gestational age embryos revealed a supernumerary notochordal-like mass of cells or a deflected notochord in association with duplication of the neural tube in mice of the duplicitas posterior stock. The perinotochordal sheath and basement membrane of the accessory notochordal masses were frequently defective. Patch and Tail-short embryos were also utilized for study by means of light rnicroscopy using Alcian blue staining. In Patch embryos, although the notochord was sometimes compressed dorso-ventrally . it had an intact perinotochordal sheath and a defined, but undulated, basement membrane. Mesenchymal cells between the notochord and neural tube were occasionally replaced by cell-free space. In contrast, in Tail-short embryos a poorly formed, lightly staining or totally absent notochordal sheath was revealed. Indeed, it was sometimes difficult to distinguish the notochord from surrounding mesenchymal cells. In both the Patch and Tail-short embryos the notochord was also deflected from its media1 position. In the three mutants studied, the director indirect effect of gene action appeared to be on the notochord and perinotochordal sheath. and the important role of these structures in abnormal axial development was established.
Open Access
Structural and ultrastructural hepatic changes in experimental canine leishmaniasis
(Murcia : F. Hernández, 1988) González, J.L.; Rollán, E.; Novoa, C.; Castaño, M.
Six 4 month-old beagles were inoculated with Leishmania donovani infantum, three of them intraperitoneally (Group A) and the other three intravenously (Group B). The animals from Group A were killed 109, 433 and 592 days after inoculation and animals from Group B 109, 171 and 334 after inoculation. The liver of each of them was examined by means of light and electron microscopy. The lesions observed in both groups were very similar, but developed more rapidly in Group B. A chronic hepatitis appeared due to infection, characterized by the presence of multiple intralobular granulomas and portal inflammatory infiltrates consisting of lymphocytes, plasmocytes and macrophages with a variable number of amastigotes. The Kupffer cells were hyperplastic and contained parasites in their cytoplasm. Gradually the hepatocytes developed a progressive cellular swelling. which during the end-stages of the process showed itself with severe nuclear degeneration, disintegration of cytoplasmic organelles, enlargement of the cytoplasmic matrix and disruption of the plasma membranes, leading to cytolysis.

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