Histology and histopathology Vol.10, nº 4 (1995)
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- PublicationOpen AccessCentral projections from the goldfish pineal organ traced by HRP-immunocytochemistry(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 1995) Jiménez, A.; Fenández Llebrez, P.; Pérez Fígares, J. M.Pineal efferent projections have been traced in the brain of the goldfish (Carassius auratus) by administration of a concentrated solution of horseradish peroxidase onto the pineal organ. After different survival times, fish were sacrificed and the administered peroxidase was revealed by immunocytochemistry on paraffin sections using an anti-horseradish peroxidase antiserum. Immunoreactive fibres were seen in the anterior hypothalamus, habenula, dorsal thalamus, ventral thalamus, optic tectum, torus longitudinalis, area pretectalis, torus semicircularis and dorsal tegmentum. No immunoreactive cell bodies were visualized in the central nervous system, thus suggesting the absence of central pinealopetal innervation. Since all areas showing pineal labelled fibres are also known to receive retinal inputs, it can be suggested that an overlapping of information from retinal and extraretinal photoreceptors may be important to processes depending on photic stimulation such as entrainment of circadian rhythms or photoneuroendocrine responses.
- PublicationOpen AccessCharacteristics of distribution of peptide-containing nerve fibres in the atrioventricular valves of the rat(Murcia : F. Hernández, 1995) Tsumori, T.; Domoto, T.; Yasui, Y.The distribution of vasoactive intestinal polypeptide-, neuropeptide Y-, and calcitonin generelated peptide-irnrnunoreactive nerve fibres was investigated in the atrioventricular valves of the rat. These nerve fibres were visualized by imrnunostaining of whole-mount preparations by the avidin-biotinperoxidase cornplex method. Vasoactive intestinal polypeptide-irnmunoreactive nerve fibres were observed mainly in the anterior cusp of the mitral valve and, to a lesser extent, in the media1 cusp of the tricuspid valve. Numerous neuropeptide Y-immunoreactive nerve fibres were found covering al1 of the cusps. Both types of peptidergic nerve fibre formed dense networks that consisted of interlacing and anastomosing nerve fibres. Calcitonin gene-related peptide-imrnunoreactive nerve fibres were seen in every cusp, but did not fom a fine network. These results provide detailed anatomical information for evaluation of the possible roles of each type of peptide-containing nerve fibre in the function of atrioventricular valves.
- PublicationOpen AccessMorphological changes of myoepithelial cells of mouse lacrimal glands during postnatal development(Murcia : F. Hernández, 1995) Wang, Y.L.; Tan, Y.; Satoh, Y.; Ono, K.To reveal the correlation between myoepithelial cell configuration and sizelshape of glandular endpieces (acini), we observed postnatal developmental changes of myoepithelial cells in the lacrimal glands of mice. Glandular and myoepithelial cells were examined in paraffin sections and in isolated acini. In newborns, rudiments of acini showed no clear lumina and glandular cells had few secretory granules. There were no myoepithelial cells with actin. At 3 days after birth, some rudiments showed lumina; however, secretory granules were not salient. Round cells in the periphery of the acini showed immunoreactivity for actin. In l-weekold mice, glandular cells were polarized: luminal cytoplasm contained some secretory granules, and nuclei were located basally. Most myoepithelial cells were flattened and sometimes projected thin processes in various directions. At 2 weeks, the glandular cells increased their size and contained numerous secretory granules, and the myoepithelial cells were almost stellate. In 4-8 week-old mice, acini increased their size, and myoepithelial cells were very thin and processes were prolonged in length and increased in number. During postnatal development, their distribution of myoepithelial cells was more scarce, while the size of acini increased. This reciprocal relation of myoepithelial cell distribution and acinar size may indicate that the changes of myoepithelial cell configurations depend on the change of acinus size.
- PublicationOpen AccessCharacteristics of MHC antigen expression and tumor-infiltrating mononuclear cells in renal cell adenomas and carcinomas(Murcia : F. Hernández, 1995) Ohmori, Takaaki; Okada, K.; Arita, N.; Watanabe, Y.; Miyazaki, T.; Tabei, RyoWe compared the expression of major histocompatibility complex (MHC; HLA class 1 and 11) antigens and the presence of tumor-infiltrating mononuclear cells presenting S 100 protein (S 100), CD68 antigen, or CD45RO antigen in formalin-fixed, paraffin-embedded tissue sections of 10 renal cell carcinomas and 9 renal cell adenomas using immunohistochemistry. The expression of B2-microglobulin (B2MG) as an HLA class 1 antigen in al1 10 cases (100%) and that of HLA-DR/a as an HLA class 11 antigen in 7 of 10 cases (70%) of carcinoma was stronger than that in the adjacent proximal convoluted tubule, but was respectively not different to weaker in 8 of 9 cases and not different to markedly weaker in al1 cases of adenoma. Furthermore, there was comparatively dense infiltration by S 100(+) antigen-presenting cells in the carcinomas, but almost none in the adenomas and generally dense infiltration by CD45RO(+) T cells and CD68(+) macrophages in the carcinomas, but little to none in the adenomas. We concluded that the generaily enhanced expression of MHC antigens in carcinomas must be an immunophenotypic deviation from not only the adjacent proximal convoluted tubule but also adenomas, and that the predominant infiltration of antigen-presenting cells, T cells and macrophages in the carcinomas, but not in the adenomas, reflects the anticancer immune reaction
- PublicationOpen AccessA genetic model for undifferentiated cell tumor formation: similar tumors formed by different cell lines transformed by the EIA oncogene(Murcia : F. Hernández, 1995) Ramón y Cajal, S.; Sánchez-Prieto, R.; Anaya, A.The activation of oncogenes and the mutation/deletion of supressor genes may be involved in tumor heterogeneity. In an attempt to study tumor heterogeneity, we transformed cell lines from epithelial (PAM 212), mesenchymal (NIH-3T3), or melanocytic origin (L-BIOBR) with the wild type Ela oncogene. To make the cell lines tumorigenic, cells were infected with Harvey sarcoma virus carrying the v-H-ras oncogene. The transformed cells were injected into nude mice and the tumors studied by optical and electron microscopy. The tumors formed by v-H-ras-transformed cells consisted of epitheliod melanomas, spindle cells sarcomas and poorly-differentiated carcinomas, depending on the cell of origin. In contrast, the tumors obtained from cells also carrying the Ela oncogene showed a predominant small and undifferentiated cell pattem regardless of the cell of origin. We conclude that the Ela oncogene products induce a negative control of differentiation, independent of the cell type, and that tumors formed by cells carrying the Ela oncogene display an undifferentiated cell pattem.