Publication: A genetic model for undifferentiated cell tumor
formation: similar tumors formed by different
cell lines transformed by the EIA oncogene
Authors
Ramón y Cajal, S. ; Sánchez-Prieto, R. ; Anaya, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The activation of oncogenes and the
mutation/deletion of supressor genes may be involved in
tumor heterogeneity. In an attempt to study tumor
heterogeneity, we transformed cell lines from epithelial
(PAM 212), mesenchymal (NIH-3T3), or melanocytic
origin (L-BIOBR) with the wild type Ela oncogene. To
make the cell lines tumorigenic, cells were infected with
Harvey sarcoma virus carrying the v-H-ras oncogene.
The transformed cells were injected into nude mice and
the tumors studied by optical and electron microscopy.
The tumors formed by v-H-ras-transformed cells
consisted of epitheliod melanomas, spindle cells
sarcomas and poorly-differentiated carcinomas,
depending on the cell of origin. In contrast, the tumors
obtained from cells also carrying the Ela oncogene
showed a predominant small and undifferentiated cell
pattem regardless of the cell of origin. We conclude that
the Ela oncogene products induce a negative control of
differentiation, independent of the cell type, and that
tumors formed by cells carrying the Ela oncogene
display an undifferentiated cell pattem.
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