Browsing by Subject "Ovariectomy"

Now showing 1 - 5 of 5
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    Comparative analysis of acute-phase protein profiles in cats undergoing ovariectomy: laparoscopic vs. conventional surgery in short time after procedure
    (MDPI, 2024-11-14) Cuervo, Belén; Satué, Katy; Velasco Martínez, María Gemma; Sopena, Joaquín Jesús; Carrillo, José María; Damiá, Elena Deborah; Chicharro, Deborah; Peláez, Pau; Martins, Emma; García Martínez, Juan Diego; Rubio, Mónica; Medicina y Cirugía Animal
    Acute-phase proteins (APPs) are biomarkers of systemic inflammation, which allow monitoring the response to surgery and post-operative complications. Ovariectomy (OVE) can be a useful model to evaluate surgical trauma and inflammation in feline species. The objective of this study was to evaluate and compare the acute-phase response (APR) after applying two different OVE techniques by measuring serum levels of serum amyloid A (SAA), haptoglobin (Hp), total serum proteins (TSPs), albumin (ALB), and paraoxonase-1 (PON-1) during the first 48 h post-surgery (hospital stay). Forty-two female cats were selected for elective spaying. The animals were randomly distributed into two groups: laparoscopic OVE (L-OVE) and midline OVE (M-OVE). Serum SAA, Hp, TSPs, ALB, and PON-1 were measured before surgery and 1, 6, 12, 24, and 48 h post-surgery. Unlike negative APPs such as PON-1 and ALB, SAA is the most sensitive positive APP for monitoring the inflammatory response in cats undergoing OVE following laparoscopic and conventional surgery. OVE performed in cats via both laparoscopic and midline techniques results in a significative increase in SAA levels, with early recovery, minor variations in serum total protein, and no significant changes in albumin, haptoglobin, or PON-1 over a 48-h period post-procedure. Based on SAA concentrations, the response to surgical trauma does not differ between surgical techniques.
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    Mandibular bone alterations of ovariectomized rats under vitamin D insufficiency
    (Murcia : F. Hernández, 2008) Said, Faika; Ghoul-Mazgar, Sonia; Ruhin, Blandine; Abdellaoui, Mohieddine; Chlaghmia, Faycel; Safta, Sihem; Guezguez, Leila; Saidane-Mosbahi, Dalila; Khemiss, Fathia
    Experimental osteoporosis was studied in mandible bone by means of ovariectomy and vitamin D insufficiency. METHODS: 42 female Wistar rats were divided into the following four groups: (1) ovariectomized rats maintained in 12h day-night light conditions (ov-l), (2) ovariectomized rats maintained in 24h dark light conditions (ov-ob), (3) sham-operated rats maintained in 12h day-night light conditions (ch-l) and (4) sham-operated rats maintained in 24 h dark conditions (ch-ob). 12 weeks later the animals were sacrificed, the mandibles were excised, cleaned and weighed, the right side of the mandibles were histologically examined and the left side of the mandibles were prepared for mineral phase analysis by X-ray diffraction. Immunohistochemical analysis was performed to detect apoptotic cells by anti-PARP p85 antibody. RESULTS: In group 2, the weight of mandibles significantly decreased. Chondroid areas were observed in ovariectomized groups and polarized light observation validated the collagen distribution disturbance in these groups (groups 1 and 2). Apoptotic osteoblasts were localized in groups 1, 2 and 4. They were numerous in group 2. The mineral phase analysis did not find differences between the groups. CONCLUSION: This study validates a new model of osteoporotic animal associating estrogens deficiency and vitamin D insufficiency where matrix synthesis and osteoblast biology are altered, but not biomineralization.
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    Ovariectomy-induced changes in the adrenal cortex of spontaneously hypertensive rats
    (Murcia : F. Hernández, 1991) Stachowiak, A.; Nussdorfer, G.G.; Malendowicz, L.K.
    Many lines of evidence indicate that adrenocortical steroid hormones are involved in the development and maintenance of hypertension in spontaneously hypertensive rats (SHR). Twenty-eight days after ovariectomy a notable decrease in the sistolic blood pressure (BP) was found in SHR, along with a marked atrophy of their adrenal cortices. The hypothesis is advanced that the ovariectomy-induced lowering in BP in SHR may be, at least partly, mediated by the suppression of the adrenal secretory activity, due to the lack of circulating estrogens, which are well known to stimulate hypophyseal ACTH release.
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    The effect of oestradiol and neta on immunohistochemical staining of iNOS and eNOS in coronary arteries of ovariectomized rats
    (Murcia : F. Hernández, 2006) Koyuncu, F.M.; Ozbilgin, K.; Kuscu, N.K.; Inan, S.; Vatansever, S.; Ceylan, E.
    Aim: The postmenopausal period is associated with increased risk for coronary atherosclerosis, and the effect of hormone replacement therapy in reducing this risk is controversial. Previous studies reported that nitric oxide synthetase (NOS) level might be important for the development of atherosclerosis, but no study has shown the interaction between hormone replacement therapy and endothelial NOS and inducible NOS intensity on coronary arteries yet. Our goal was to find out the immunostaining intensity of endothelial NOS and inducible NOS in ovariectomized rats which received oestradiol and norethisterone treatment. Methods: We performed bilateral ovariectomy in 15, female, 90-day-old Wistar rats with an average weight of 250 grams. After waiting for 4 weeks for the menopausal state, they were divided into 3 groups to receive either placebo, 0.1 mg/day 17-ßoestradiol (group E2), or 0.1 mg/day 17-ß-oestradiol + 0.1 mg/day norethisterone acetate (group E2-NETA) for 5 weeks. Another group included 5, normal, adult, female intact rats and served as controls. At the end of the treatment, all rats were sacrificed and coronary arteries were stained with inducible NOS and endothelial NOS polyclonal antibodies using streptavidin-biotin technique. Results: The immunostaining of inducible NOS was prominent in perivascular connective tissue of the ovariectomized group but not in the control group. The inducible NOS immunostaining immunoreactivity was not detected in either treated groups. Immunostaining intensity of endothelial NOS did not differ in any 4 groups with similar staining. Conclusion: The present findings indicate that hormone replacement therapy down-regulates iNOS expression in coronary arteries of ovariectomized rats, and reduced iNOS may likely be involved in estrogen’s beneficial effects.
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    Young coconut juice significantly reduces histopathological changes in the brain that is induced by hormonal imbalance: A possible implication to postmenopausal women
    (Murcia : F. Hernández, 2009) Radenahmad, Nisaudah; Saleh, Farid; Sawangjaroen, Kitja; Rundorn, Wilart; Withyachumnarnkul, Boornsirm; Connor, James R.
    Background and aim: Some degenerative diseases of the nervous system have been linked to hormonal imbalance in postmenopausal women. It is argued that young coconut juice (YCJ) could have some estrogen-like characteristics, but this is still debatable. Our aim was to investigate this argument, and to examine whether YCJ has any neuroprotective effects. Materials and methods: Four groups of female rats (10 in each group) were included in this study. These included sham-operated, overiectomized (ovx), ovx and receiving estradiol benzoate (EB) injections intraperitoneally, and ovx and receiving YCJ orally. At the end of the five-week study, the rats were sacrificed, and their serum estradiol (E2) level was measured by chemiluminescent immunoassay. Moreover, the rat brains were excised, and the cortical pyramidal neurons were examined using markers of neuronal cell death, namely anti-neurofilament (NF200) and antiparvalbumin (PV) antibodies. Results: Our results showed that the rat group which received YCJ had its serum E2 level significantly (P<0.05) higher than the ovx group which did not receive any treatment, and the sham-operated group. A similar trend was observed with the group which received EB injections, but no significant difference was present when the latter was compared with the shamoperated group. In addition, a significant reduction in neuronal cell death was observed in the YCJ-treated group, as compared to the ovx group which did not receive any treatment. This was indicated by the significantly (P<0.05) higher number of neurons which were immunopositive for NF200 and PV. Interestingly, the number of these neurons was also significantly (P<0.05) higher in the YCJ group, as compared to the EB group. Conclusion: This study confirms the argument that YCJ has estrogen-like characteristics, and it also adds more evidence to the observation that hormonal imbalance could induce some brain pathologies in females.