Publication: Mandibular bone alterations of ovariectomized rats under vitamin D insufficiency
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Date
2008
Authors
Said, Faika ; Ghoul-Mazgar, Sonia ; Ruhin, Blandine ; Abdellaoui, Mohieddine ; Chlaghmia, Faycel ; Safta, Sihem ; Guezguez, Leila ; Saidane-Mosbahi, Dalila ; Khemiss, Fathia
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Experimental osteoporosis was studied in
mandible bone by means of ovariectomy and vitamin D
insufficiency. METHODS: 42 female Wistar rats were
divided into the following four groups: (1)
ovariectomized rats maintained in 12h day-night light
conditions (ov-l), (2) ovariectomized rats maintained in
24h dark light conditions (ov-ob), (3) sham-operated rats
maintained in 12h day-night light conditions (ch-l) and
(4) sham-operated rats maintained in 24 h dark
conditions (ch-ob). 12 weeks later the animals were
sacrificed, the mandibles were excised, cleaned and
weighed, the right side of the mandibles were
histologically examined and the left side of the
mandibles were prepared for mineral phase analysis by
X-ray diffraction. Immunohistochemical analysis was
performed to detect apoptotic cells by anti-PARP p85
antibody. RESULTS: In group 2, the weight of
mandibles significantly decreased. Chondroid areas were
observed in ovariectomized groups and polarized light
observation validated the collagen distribution
disturbance in these groups (groups 1 and 2). Apoptotic
osteoblasts were localized in groups 1, 2 and 4. They were numerous in group 2. The mineral phase analysis
did not find differences between the groups.
CONCLUSION: This study validates a new model of
osteoporotic animal associating estrogens deficiency and
vitamin D insufficiency where matrix synthesis and
osteoblast biology are altered, but not biomineralization.
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