Publication: The effect of oestradiol and neta on immunohistochemical staining of iNOS and eNOS in coronary arteries of ovariectomized rats
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Date
2006
Authors
Koyuncu, F.M. ; Ozbilgin, K. ; Kuscu, N.K. ; Inan, S. ; Vatansever, S. ; Ceylan, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Aim: The postmenopausal period is
associated with increased risk for coronary
atherosclerosis, and the effect of hormone replacement
therapy in reducing this risk is controversial. Previous
studies reported that nitric oxide synthetase (NOS) level
might be important for the development of
atherosclerosis, but no study has shown the interaction
between hormone replacement therapy and endothelial
NOS and inducible NOS intensity on coronary arteries
yet. Our goal was to find out the immunostaining
intensity of endothelial NOS and inducible NOS in
ovariectomized rats which received oestradiol and
norethisterone treatment. Methods: We performed
bilateral ovariectomy in 15, female, 90-day-old Wistar
rats with an average weight of 250 grams. After waiting
for 4 weeks for the menopausal state, they were divided
into 3 groups to receive either placebo, 0.1 mg/day 17-ßoestradiol
(group E2), or 0.1 mg/day 17-ß-oestradiol +
0.1 mg/day norethisterone acetate (group E2-NETA) for
5 weeks. Another group included 5, normal, adult,
female intact rats and served as controls. At the end of
the treatment, all rats were sacrificed and coronary
arteries were stained with inducible NOS and endothelial
NOS polyclonal antibodies using streptavidin-biotin technique. Results: The immunostaining of inducible
NOS was prominent in perivascular connective tissue of
the ovariectomized group but not in the control group.
The inducible NOS immunostaining immunoreactivity
was not detected in either treated groups.
Immunostaining intensity of endothelial NOS did not
differ in any 4 groups with similar staining. Conclusion:
The present findings indicate that hormone replacement
therapy down-regulates iNOS expression in coronary arteries of ovariectomized rats, and reduced iNOS may
likely be involved in estrogen’s beneficial effects.
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