Browsing by Subject "Adenosine deaminase"
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- PublicationOpen AccessAdenosine deaminase, not immune to a mechanistic rethink in central nervous system disorders?(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2022) Hall, Benjamin; George, Jonathan G.; Allen, Scott P.Adenosine deaminase (ADA) is a purine metabolism enzyme that catalyses the breakdown of adenosine and deoxyadenosine. The enzyme is important in several cellular processes, including the innate immune response and cellular differentiation, and it is also an important enzyme for the maintenance of brain homeostasis, in part due to its regulation of adenosine. Aberrant regulation of ADA enzyme activity has been linked to several neurodegenerative diseases and diseases that can result in neurological impairment. However, the mechanisms behind altered ADA regulation and how this leads to the development of neurological dysfunction are poorly characterised. This review summarises the current research on ADA and its role and regulation in disease pathology, with a focus on the central nervous system (CNS) and the neurodegenerative disease, amyotrophic lateral sclerosis (ALS)
- PublicationOpen AccessChanges in serum biomarkers of inflammation in bovine besnoitiosis(2021-09-22) González Barrio, David; Huertas López, Ana; Diezma Díaz, Carlos; Ferre, Ignacio; Cerón Madrigal, José Joaquín; Ortega Mora, Luis Miguel; Álvarez García, Gema; Medicina y Cirugía Animal
- PublicationOpen AccessDifferences in weight, hierarchy, and incidence of lameness between two groups of adult pigs derived from assisted reproductive technologies(MDPI, 2022-12-17) Ramírez, Lisette L.; Ortin Bustillo, Alba; Ramis, Guillermo; Romar, Raquel; Coy, Pilar; Romero Aguirregomezcorta, Jon; Ramírez, Lisette L.; Ortín Bustillo, Alba; Ramis Vidal, Manuel Guillermo; Romar Andrés, Raquel; Coy Fuster, Pilar; Anatomía y Anatomía Patológica Comparadas ; Facultad de VeterinariaThe in vitro production (IVP) and subsequent transfer of embryos (ET) to recipient mothers is not yet an established reproductive technology in the pig industry, as it is in cattle. However, that the trade of IVP-cryopreserved pig embryos is expected to start in the next decades. Society and governments are increasingly aware of the repercussions that IVP could have for animal health, welfare, behavior, or food safety, but proven scientific information for this type of animal does not exist, since no colonies of pigs have been created to this end. We created a small one and studied the differences between 16 IVP-derived pigs and 14 pigs derived from artificial insemination (AI), at 3.5 years of age, conceived from the same boar, and housed and fed under the same conditions since they were born. Incidence of lameness, position in the herd hierarchy, weight, adenosine deaminase activity, and hematological and biochemical analytes were compared between the two groups of animals. The results showed that the IVP animals weighed more, occupied higher positions in the herd hierarchy, and had a lower incidence of lameness. Although genetic differences from the maternal line could explain some of these results, it is also possible that the IVP animals developed better adaptative abilities, but more studies with a higher number of animals are necessary to reach consistent conclusions.
- PublicationEmbargoSalivary adiponectin, but not adenosine deaminase, correlates with clinical signs in women with Sjögren's syndrome: a pilot study(2019-03) López Jornet, María Pía; Tvarijonaviciute, Asta; Zamora, Carmen; Martinez Subiela, Silvia; Tecles, Fernando; Pina, Francisca; Dermatología, Estomatología, Radiología y Medicina FísicaAbstract Objectives: To evaluate salivary adiponectin and adenosine deaminase (ADA) in women suffering from Sjögren's syndrome (SS). Methods: Salivary adiponectin and ADA were measured in patients with SS (n = 17) and compared to their values in healthy controls (n = 13) and patients suffering from drug-induced xerostomia (non-SS sicca group; n = 19). A clinical history was made for each patient, patients were examined clinically, and xerostomia inventory (XI) was performed. Results: Salivary adiponectin corrected by total protein was higher in patients with SS than in healthy individuals (P < 0.05) or patients with non-SS sicca (P < 0.01) and correlated with XI (r = 0.555; P < 0.05). Salivary ADA was higher in patients with SS and non-SS sicca compared to controls (P < 0.05 in both cases). Conclusion: The results of the present study indicate that adiponectin and ADA are increased in the saliva of patients with SS. Clinical relevance: Salivary adiponectin corrected by total protein can be a potential biomarker of SS. Trial registration: NCT03156569.
- PublicationRestrictedSalivary adiponectin, but not adenosine deaminase, correlates with clinical signs in women with Sjögren’s syndrome: a pilot study(https://link.springer.com/, 2018-07-20) Tvarijonaviciute, Asta; Zamora, Carmen; Martinez-Subiela, Silvia; Tecles, Fernando; Pina, Francisca; Lopez Jornet, Pia; Dermatología, Estomatología, Radiología y Medicina FísicaObjectives To evaluate salivary adiponectin and adenosine deaminase (ADA) in women suffering from Sjögren’s syndrome (SS). Methods Salivary adiponectin and ADA were measured in patients with SS (n = 17) and compared to their values in healthy controls (n = 13) and patients suffering from drug-induced xerostomia (non-SS sicca group; n = 19). A clinical history was made for each patient, patients were examined clinically, and xerostomia inventory (XI) was performed. Results Salivary adiponectin corrected by total protein was higher in patients with SS than in healthy individuals (P < 0.05) or patients with non-SS sicca (P < 0.01) and correlated with XI (r = 0.555; P < 0.05). Salivary ADAwas higher in patients with SS and non-SS sicca compared to controls (P<0.05 in both cases). Conclusion The results of the present study indicate that adiponectin and ADA are increased in the saliva of patients with SS. Clinical relevance Salivary adiponectin corrected by total protein can be a potential biomarker of SS.
- PublicationOpen AccessUse of adenosine deaminase (ADA) as a biomarker for lameness and growth speed in pigs(Springer Nature, 2022) Ramirez, Lisette; Romar, Raquel; Canovas, Sebastian; Ortin, Alba; Coy, Pilar; Romero Aguirregomezcorta, Jon; FisiologíaAdenosine deaminase 2 (ADA) is an enzyme considered a biomarker of the immune system, which is found in serum, saliva, and lymphoid tissue. A direct relationship between ADA in saliva and the immune status of the animal has been shown, but there are few studies on the use of ADA as a biomarker for growth rate or lameness. Here, a colony of pigs (N=27, 22 females and 5 males, Large-White x Landrace living in semi-free conditions in a sanctuary located at the Teaching Farm of the University of Murcia (Spain) was used. From birth to the present, their individualized growth curves were recorded. At 3.5-year age, saliva samples were taken to detect ADA, and the animals suffering from lameness, one of the most frequent pathologies in the pig sector, were identified. ADA measurement was performed using a commercial automated spectrophotometric assay (Diazyme Laboratories, Poway, CA, USA) adapted to the Olympus AU600 analyzer, a validated method for pig saliva. Statistical analysis was performed in ``R´´ version 4.0.3 (R Core Team 2020). The variables used for the analysis were ADA2 values in saliva and presence/absence of lameness at the age of 3.5 years, and average daily weight gain (ADG) () at different age points. Spearman's correlation coefficient (s) was used to measure the correlations between the variables. The results showed a negative correlation (s=-0.4) between ADA values and ADG in the period between days 45 and 90. These ADA values could indicate a greater predisposition to diseases in animals with low ADG at that time due to a worse immune status. No correlation with the ADA values was found for the incidence of lameness (s=-0.19). However, a strong relationship was observed between ADG in 90-135 days and the incidence of lameness at 4 years of age (s=0.52). Likewise, positive correlations were observed between ADG at 0-45 days (s=0.45), 45-135 (s=0.46) and 0-135 days (s=0.47) and the incidence of lameness at 4 years. These data indicate that rapid growth rate during the first 6 months of life predisposes animals to lameness in adulthood.