Histology and histopathology Vol. 3, nº 1 (1988)
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- PublicationOpen AccessUltrastructural pathologic changes of rat extraembryonic visceral endodermal cells exposed to teratogenic antibodies in vivo(Murcia : F. Hernández, 1988) Leung, C.C.K.; DeSha, D.L.; Bui, L.; Cheewatrakoolpong, B.
- PublicationOpen Accesslmmunocytochemical localization of parathyroid hormone in rabbit parathyroid glands(Murcia : F. Hernández, 1988) Shizuko Shoumura; Noriko Ishizaki; Shoichi Emura; Yuji Iwasaki; Tomo Yamahira; Hideo IsonoImmunocytochemical localization of parathyroid hormone was examined in the rabbit parathyroid gland by means of protein A-gold technique. Protein A-gold particles were observed on the secretory granules and the large secretory granules thought to be storage granules. No protein A-gold particles were observed on cisternae of the endoplasmic reticulum and the Golgi apparatus
- PublicationOpen AccessUltrastructural computerized morphometry of platelets in chronic myelogenous leukemia making use of ultra-thin sections and freeze-fracture procedure a preliminary approach(Murcia : F. Hernández, 1988) Bianciardi, G.; Battistelli, S.; Toti, P.; Vallesi, G.; Weber, G.Biochemical and morphological data have shown that the circulating platelets are deeply altered in patients with chronic mielogenous leukemia. In this report we describe the results of ultrastructural morphometry performed by means of a computerized device of the platelet shape (ultra-thin sectioned platelets) and of the platelet plasma-membrane (freezefractured platelets). Platelets appeared deeply modified: reduced mean platelet area and perimeter, increased surface density of the openings of the surface connected canalicular system, abnormal features of the platelet cytoplasm and some aspects of platelets joined together even if with heterogeneity in these findings did appear from patient to patient.
- PublicationOpen AccessHepatitis B core and surface antigens and delta agent in chronic liver disease in Kuwait(Murcia : F. Hernández, 1988) Al-Adnani, M. S.
- PublicationOpen AccessDistribution of ferric iron in larval lampreys, Petromyzon marinus L(Murcia : F. Hernández, 1988) Hall, S.J.; Youson, J.H.The distribution and abundance of ferric iron in larval lampreys (Petromyzon marinus L.) were investigated using light microscopy and the Prussian blue stain. Animals from various watersheds contained different concentrations of iron, although the sites of deposition were the same for all animals. A major portion of iron is within adipose tissue. while the liver, and cartilage contain predominantly low to trace amounts of iron. respectively. Iron is associated with fibrous connective tissue in several places in the body, and this association may have particular significance in the inner ear. Iron is also located in cells of the meninges. The presence of iron in the epithelia1 cells of the posterior intestine may reflect elimination of the metal through the extrusion of iron-loaded cells into the intestinal lumen. Iron within mucous cells of the epidermis, suggest elimination of iron during mucous secretion. Iron-loaded cells of bipolar shape are also present in the epidermis, but are particularly prominent around the branchiopore. Low concentrations of iron are observed within in melanin-containing macrophages (melano-macrophages) in regions of iron absorption, erythrophagocytosis, and haemopoiesis. High levels of iron in the epithelia and lumina of pronephric tubules are concomitant with degeneration of this organ. These data are evidence of the wide spread distribution of iron in lamprey tissues and additional evidence for the potential value of lampreys for the study of iron metabolism in vertebrates.
- PublicationOpen Accesslmmunophenotypic characterization of primary and secondary lymphoid follicles(Murcia : F. Hernández, 1988) Luaces, César P. de; Peral, José I.; García Tejeiro, Manuel; Aguirre, CésarThc need for an immunophenotypical referential framework relative to lymphoid follicle has led us to apply a panel of monoclonal and polyclonal antibodics, by means of a sensitive immunostaining method. Lymphoid follicle is an immunophenotypically complex structure made up of three lymphoid populations (B, being its bulk, and a few T and NK cells). dcndritic reticulum cells (DRCs) and Flemming's macrophagcs. Follicular B population is To 15 +. B1 + , OKB 7 +, HLA-DR + and C3bR +. In secondary follicles therc are differential characteristic reactivities for cach topographic compartment: Mantle zone is positive for OKB 2 and surface IgM (sIgM) and IgD (sIgD): germinal center (GC) clear zone (with centrocytic predominance) for OKT 9, sIgM and weakly for OKB 2: and GC dark zone (with centroblastic predoiniiiance) only for OKT 9. In sections, OKT 10 allows one to see immunoblasts and plasma cells, the latter being with lymphoplasmacytoid cells the only intracytoplasmic immunoglobulin holders. 10% of GC lympliocytcs are T cells, almost exclusively T-helper (Leu 3a +). Another 10% to 15% of lymphoid cells are Leu 7 (HNK- 1) +. In histological sections, DRCs are spccifically marked with R4123 and Flemming's inacrophages with anti-alpha,-antitrypsin and antialpha,- antichymotrypsin antibodies, both populations bcing negative to OKM 1 and OKM 5.
- PublicationOpen AccessNeuron specific enolase-positive breast carcinomas(Murcia : F. Hernández, 1988) Erikstein, Bjerrn; Nesland, Jahn M.; Ottestad, Lars; Lund, Eiliv; Johannessen, Jan VicentsNinety-eight patients treated for breast carcinomas were followed from 54 to 75 months after primary diagnosis. All had undergone a modified radical mastectomy with removal of axillary lymph nodes. 36 breast carcinomas were NSE-positive and 62 were negative. NSE-positive tumours were significantly more frequently estrogen receptor-positive than the NSEnegative tumours, and the estrogen receptor values were higher in the NSE-positive groups. Patients with NSE-positive tumours and patients with NSE-negative tumours did not differ with regard to presence of lymph node metastases at the time of primary surgery. However, the study showed that patients with NSE-positive tumours had a tendency towards more lymph node metastases after primary surgical intervention, but a better outcome than patients with NSE-negative tumours and metastases. This study, with a 5-year follow up, failed to demonstrate any major prognostic significance of immunostaining for NSE.
- PublicationOpen Accesslmmunohistochemical study of Warthin's tumour with special regard to the germinal centre(Murcia : F. Hernández, 1988) Akihiro Masuda
- PublicationOpen AccessRetinal epithelial fine structure in the vervet monkey (Cercopithecus aethiops)(Murcia : F. Hernández, 1988) Braekevelt, Charlie R.The morphology of the retinal pigment epithelium (RPE) and closely associated Bruch's membrane (complexus basalis) and choriocapillaris have been investigated by electron microscopy in the vervet monkey (Cercopithecus aethiops). The RPE is composed of a single layer of cuboidal cells joined laterally by apically-located junctional complexes. Basally (sclerally) these cells display numerous infoldings while apically (vitreally) abundant processes enclose and interdigitate with rod outer segments. Internally the large vesicular nucleus is centrally located and smooth endoplasmic reticulum. mitochondria and lysosome-like bodies. are plentiful. Rough endoplasmic reticulum, polysomes and melanosomes while present are not abundant. Phagosomes of outer segment discs are noted in various stages of uptake and degradation. The choriocapillaris is highly fenestrated over large areas. Bruch's membrane shows the typical pentalaminate structure noted in other mammalian species without a tapetum lucidum.
- PublicationOpen AccessSquamous and glandular differentiation in urothelial bladder carcinomas. Histopathology, histochemistry and immunohistochemical expression of carcinoembryonic antigen(Murcia : F. Hernández, 1988) López-Beltrán, A.; Martín, J.; García, J.; Toro, ManuelThis paper reports the itiin~~~nochemical expression of carcinoembryonic antigen in the s c l u a n~o~~glsa.n dular anci mixed clifferrentiation areas ohser\.cd in l90 ~~rothe l iuarli nary bladder carcinomas. Tlic antigen kvas found to occur in 7596 and 81 96 of all papillary and solid infiltrating carcinomas. rcspectivcly. Thc use of thc imm~~nohistochemicdael termination of CEA in improving the niorphological examination of these cliffcrcntiation arcas and their scant nrescnce in thc prognosis of urothelial carcinomas involbing squun~ous andlor glandular focal differentiation are discussed
- PublicationOpen AccessEffects of orthodontic tooth movement on the alcian blue staining patterns of rat alveolar bone,an histochemical study(Murcia : F. Hernández, 1988) Martínez, R.H.; Johnson, R. B.
- PublicationOpen AccessA light and electron microscopic study of the preoptic nucleus of the grass frog. Rana pipiens, under normal conditions and following transection of the preoptico-neurohypophysial tract(Murcia : F. Hernández, 1988) Al-Tikriti, M.A.; Henry, R. W.