Publication: lmmunophenotypic characterization of primary and secondary lymphoid follicles
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Date
1988
Authors
Luaces, César P. de ; Peral, José I. ; García Tejeiro, Manuel ; Aguirre, César
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Thc need for an immunophenotypical
referential framework relative to lymphoid follicle has
led us to apply a panel of monoclonal and polyclonal
antibodics, by means of a sensitive immunostaining
method. Lymphoid follicle is an immunophenotypically
complex structure made up of three lymphoid
populations (B, being its bulk, and a few T and NK cells).
dcndritic reticulum cells (DRCs) and Flemming's
macrophagcs. Follicular B population is To 15 +. B1 + ,
OKB 7 +, HLA-DR + and C3bR +. In secondary
follicles therc are differential characteristic reactivities
for cach topographic compartment: Mantle zone is
positive for OKB 2 and surface IgM (sIgM) and IgD
(sIgD): germinal center (GC) clear zone (with
centrocytic predominance) for OKT 9, sIgM and weakly
for OKB 2: and GC dark zone (with centroblastic
predoiniiiance) only for OKT 9. In sections, OKT 10
allows one to see immunoblasts and plasma cells, the
latter being with lymphoplasmacytoid cells the only
intracytoplasmic immunoglobulin holders. 10% of GC
lympliocytcs are T cells, almost exclusively T-helper
(Leu 3a +). Another 10% to 15% of lymphoid cells are
Leu 7 (HNK- 1) +. In histological sections, DRCs are
spccifically marked with R4123 and Flemming's
inacrophages with anti-alpha,-antitrypsin and antialpha,-
antichymotrypsin antibodies, both populations
bcing negative to OKM 1 and OKM 5.
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