Publication: Palatal shelf reorientation in hamster embryos following treatment with 5-fluorouracil
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Date
1989
Authors
Shah, Ravindra M. ; Chen, Yung Ping ; Burdett, David N.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A study was undertaken to examine the issue
of whether achieving a critical mass of cells andor palatal
shelf volume during vertical development of shelf is
essential for reorientation to occur. In control and 5-
fluorouracil (5FU)-treated hamster embryos' palatal
shelves, at different times during gestation, the numbers
of both epithelial and mesenchymal cells were counted
and cross-sectional area was measured. DNA synthesis
was measured by 3H-thymidine incorporation and was
used as an index of growth by cell proliferation. The
control data indicated that, unlike development during
initial 24 hours, the later period of vertical palatal
development was characterized by a steady level of
mesenchymal and epithelial cell numbers and palatal
shelf area. Following 5FU treatment all the measurements
were reduced, and until they reached the
equivalent of control values, the palatal shelves did not
reorient. The density of mesenchymal cells in the
developing palate did not seem to affect cell number. On
the basis of the analysis of results of the present study,
along with those reported in the literature, it is suggested
that, in hamsters, acquisition and maintenance of both a
specified number of mesenchymal cells and shelf area, at
least 24 hours prior to reorientation, may be critical for
ensuing mesenchymal differentiation to enforce palatal
shelf reorientation on schedule. 5FU affected these
features to delay reorientation of the palatal shelf.
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