Publication: Human dental Pulp stem cells exhibit different biological behaviours in response to commercial bleaching products
Authors
Llena, Carmen ; Collado-González, Mar ; Tomás Catalá, Christopher Joseph ; García Bernal, David ; Oñate Sánchez, Ricardo Elías ; Rodríguez Lozano, Francisco Javier ; Forner, Leopoldo
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Publisher
MDPI
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DOI
https://doi.org/10.3390/ma11071098
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using
analysis of variance and Kruskal–Wallis or Mann–Whitney tests ( < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 x 15 min groups (p < 0.001), while 1 x 30 min of PerfectBleach and 1 x 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 x 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs.
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Citation
Materials (2018) 11(7):1098
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