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Human dental Pulp stem cells exhibit different biological behaviours in response to commercial bleaching products

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dc.contributor.authorLlena, Carmen
dc.contributor.authorCollado-González, Mar
dc.contributor.authorTomás Catalá, Christopher Joseph
dc.contributor.authorGarcía Bernal, David
dc.contributor.authorOñate Sánchez, Ricardo Elías
dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.authorForner, Leopoldo
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-09-25T07:34:41Z
dc.date.available2025-09-25T07:34:41Z
dc.date.copyright© 2018, by the authors
dc.date.issued2018-06-27
dc.description.abstractThe purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using analysis of variance and Kruskal–Wallis or Mann–Whitney tests ( < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 x 15 min groups (p < 0.001), while 1 x 30 min of PerfectBleach and 1 x 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 x 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs.
dc.formatapplication/pdf
dc.format.extent15
dc.identifier.citationMaterials (2018) 11(7):1098
dc.identifier.doihttps://doi.org/10.3390/ma11071098
dc.identifier.issn1996-1944
dc.identifier.urihttp://hdl.handle.net/10201/161430
dc.languageeng
dc.publisherMDPI
dc.relationResearch was funded by by the Spanish Net of Cell Therapy (TerCel), RETICS subprograms of the I+D+I 2013–2016 Spanish National Plan, and projects “RD12/0019/0001” and “RD16/0011/0001” funded by the Instituto de Salud Carlos III to JMM and co-funded by the European Regional Development Fund.
dc.relation.publisherversionhttps://www.mdpi.com/1996-1944/11/7/1098
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBleaching products
dc.subjectDiffusion
dc.subjectCytotoxicity
dc.subjectDental pulp
dc.subjectStem cells
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleHuman dental Pulp stem cells exhibit different biological behaviours in response to commercial bleaching products
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublication.latestForDiscoveryba32f760-d55c-40a7-9452-62dfd47cbeaf
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