Publication: Biocompatibilidad y bioactividad de materiales para terapia vital pulpar
Authors
Mora Martínez, Alejandro
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Escuela Internacional de Doctorado
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López García, Sergio ; Murcia Flores, Laura ; Rodríguez Lozano, Francisco Javier
Publisher
Universidad de Murcia
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DOI
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info:eu-repo/semantics/doctoralThesis
Description
Abstract
Objetivo: El objetivo del presente estudio fue evaluar la biocompatibilidad y bioactividad de tres materiales basados en silicato de calcio, frente a células madre de pulpa dental.
Material y Métodos: Los materiales empleados para su análisis fueron MTA Angelus, TheraCal PT y Biodentine XP. Para el estudio de las propiedades tanto de biocompatibilidad como de bioactividad se llevaron a cabo un total de 10 ensayos: análisis de la viabilidad celular (MTT) (24, 48 y 72 horas), ensayo de migración celular (24, 48 y 72 horas), inmunofluorescencia (72 horas), estudio de la morfología y adhesión celular a los materiales, espectrometría dispersiva de rayos X (EDX), análisis de la liberación de iones de los materiales (ICP-MS), ensayo de apoptosis/necrosis celular, análisis de la producción de especies reactivas de oxígeno (ROS), evaluación de la expresión de marcadores osteo-odontogénicos de las DPSCs (3 y 7 días), así como el análisis de su potencial de mineralización (21 días). El análisis estadístico se efectuó mediante ANOVA unidireccional y la prueba post-hoc de Tukey (p < 0,05).
Resultados: Los resultados muestran como TheraCal PT sin diluir presenta una menor actividad metabólica celular respecto al control, así como una menor capacidad de migración celular, en cambio MTA Angelus y Biodentine XP reflejan resultados óptimos semejantes al control. Del mismo modo los mejores resultados en cuanto a adhesión y morfología celular fueron los observados para MTA Angelus y Biodentine XP, siendo este último quien mayor cantidad de calcio mostró tras el análisis EDX, y el material con menor tasa tanto de apoptosis/necrosis celular como de liberación de especies reactivas de oxígeno. En cuanto a las propiedades bioactivas, Biodentine XP mostró una regulación significativa de los marcadores osteo-odontogénicos, ALP y DSPP, en comparación con los grupos control; así como una formación de nódulos calcificados claramente superior en comparación con el resto de materiales.
Conclusiones: Los hallazgos del estudio muestran como los materiales basados en silicato de calcio mejoran las propiedades biológicas y el potencial de mineralización de las DPSCs. Especialmente, el nuevo Biodentine XP presentó resultados especialmente favorables en comparación con el resto de materiales, aunque semejantes a los mostrados por MTA Angelus, siendo TheraCal PT el material con resultados ligeramente inferiores en cuanto a biocompatibilidad y bioactividad.
Objective: The aim of this study was to evaluate the biocompatibility and bioactivity of three calcium silicate-based materials against dental pulp stem cells. Material and Methods: The materials used for analysis were MTA Angelus, TheraCal PT and Biodentine XP. For the study of both biocompatibility and bioactivity properties, a total of 10 assays were carried out: cell viability analysis (MTT) (24, 48 and 72 hours), cell migration assay (24, 48 and 72 hours), immunofluorescence (72 hours), study of cell morphology and adhesion to the materials, X-ray dispersive spectrometry (EDX), ion release analysis of the materials (ICP-MS), cell apoptosis/necrosis assay, analysis of the production of reactive oxygen species (ROS), evaluation of the expression of osteo-odontogenic markers of the DPSCs (3 and 7 days), as well as analysis of their mineralisation potential (21 days). Statistical analysis was performed by one-way ANOVA and Tukey´s post-hoc test (p < 0,05). Results: The results show that, compared to the control, undiluted TheraCal PT exhibits lower cell metabolic activity and cell migration capacity, while MTA Angelus and Biodentine XP demonstrate results that are similar to the control. Similarly, MTA Angelus and Biodentine XP produced the best results in terms of cell adhesion and morphology. The latter showed the highest amount of calcium after EDX analysis and was the material with the lowest rate off cell apoptosis/necrosis and reactive oxygen species release. In terms of bioactivity, Biodentine XP significantly regulated the osteo-odontogenic markers ALP and DSPP compared to the control groups and clearly promoted higher calcified nodule formation than the other materials. Conclusions: The study´s findings show calcium silicate-based materials can enhance the biological properties and mineralisation potential of DPSCs. The new Biodentine XP in particular showed favourable results, similar to those MTA Angelus, though slightly lower than TheraCal PT in terms of biocompatibility and bioactivity.
Objective: The aim of this study was to evaluate the biocompatibility and bioactivity of three calcium silicate-based materials against dental pulp stem cells. Material and Methods: The materials used for analysis were MTA Angelus, TheraCal PT and Biodentine XP. For the study of both biocompatibility and bioactivity properties, a total of 10 assays were carried out: cell viability analysis (MTT) (24, 48 and 72 hours), cell migration assay (24, 48 and 72 hours), immunofluorescence (72 hours), study of cell morphology and adhesion to the materials, X-ray dispersive spectrometry (EDX), ion release analysis of the materials (ICP-MS), cell apoptosis/necrosis assay, analysis of the production of reactive oxygen species (ROS), evaluation of the expression of osteo-odontogenic markers of the DPSCs (3 and 7 days), as well as analysis of their mineralisation potential (21 days). Statistical analysis was performed by one-way ANOVA and Tukey´s post-hoc test (p < 0,05). Results: The results show that, compared to the control, undiluted TheraCal PT exhibits lower cell metabolic activity and cell migration capacity, while MTA Angelus and Biodentine XP demonstrate results that are similar to the control. Similarly, MTA Angelus and Biodentine XP produced the best results in terms of cell adhesion and morphology. The latter showed the highest amount of calcium after EDX analysis and was the material with the lowest rate off cell apoptosis/necrosis and reactive oxygen species release. In terms of bioactivity, Biodentine XP significantly regulated the osteo-odontogenic markers ALP and DSPP compared to the control groups and clearly promoted higher calcified nodule formation than the other materials. Conclusions: The study´s findings show calcium silicate-based materials can enhance the biological properties and mineralisation potential of DPSCs. The new Biodentine XP in particular showed favourable results, similar to those MTA Angelus, though slightly lower than TheraCal PT in terms of biocompatibility and bioactivity.
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