Publication: Reproductive fluids reduce the rate of telomer shortening during the first year of life in in vitro derived- pig and cattle
Authors
Garcia-Cobarro, Claudia ; Romar, Raquel ; Ramis Vidal, Guillermo ; Canovas, Sebastian ; Gutiérrez-Adán, Alfonso ; Coy, Pilar
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Publisher
Springer Nature
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DOI
https://doi.org/10.1007/s13105-022-00923-3
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info:eu-repo/semantics/other
Description
Abstract
The shortening rate of telomere length in blood cells is a potential predictive tool for health and lifespan. It is unknown whether the differences in telomere shortening observed between individuals are genetically determined or whether early environmental factors produce such differences. In this study, we analyzed the relative length of leukocyte telomeres throughout the first year of life in two animal colonies of the bovine and porcine species. The animals were generated by artificial insemination (AI) (control group) or by in vitro production (IVP) and transfer of embryos, either using conventional culture media (C-IVP, with bovine serum albumin as the only protein source) or enriched culture media (ET-IVP, with reproductive fluids as an extra source of proteins). Blood samples obtained 3 days, one month (day 28-30), 4 months (day 120), and one year (day 365) after birth were used. Cellular DNA was extracted, purified, and quantified following standardized protocols. Subsequently, the telomere length was determined by real-time quantitative PCR, normalizing the telomere amplification values with respect to the amount of the constitutive gene Rn18S (relative quantification). Statistical analysis (one way ANOVA and Bonferroni in cattle, two-sample t-test for inhomogeneous variances in pig, Pearson correlation and regression analysis) of the relative telomere size with respect to age and animal group allowed us to observe the dynamics of shortening that occurs in both species. The results showed a progressive shortening in telomere length with age, with the greatest shortening occurring in cattle at 30 days and in pigs at 120 days. As for the speed of shortening, in cattle, it was observed that the C-IVP group suffered a greater shortening between days 30 and 120 compared to the AI and ET-IVP groups. In pigs, a greater reduction was also observed in the C-IVP group than in the ET-IVP group. The results in both species suggest that supplementation with reproductive fluids during IVP reduces the rate of shortening of telomere length during the early development of individuals.
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Citation
Journal of Physiology and Biochemistry 2022, 78: pp O4-07
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