Reproductive fluids reduce the rate of telomer shortening during the first year of life in in vitro derived- pig and cattle

Garcia-Cobarro, Claudia; Romar, Raquel; Ramis Vidal, Guillermo; Canovas, Sebastian; Gutiérrez-Adán, Alfonso; Coy, Pilar

Publication:
Reproductive fluids reduce the rate of telomer shortening during the first year of life in in vitro derived- pig and cattle

dc.contributor.author	Garcia-Cobarro, Claudia
dc.contributor.author	Romar, Raquel
dc.contributor.author	Ramis Vidal, Guillermo
dc.contributor.author	Canovas, Sebastian
dc.contributor.author	Gutiérrez-Adán, Alfonso
dc.contributor.author	Coy, Pilar
dc.contributor.department	Fisiología	es
dc.date.accessioned	2025-09-05T09:56:34Z
dc.date.available	2025-09-05T09:56:34Z
dc.date.copyright	© 2022
dc.date.issued	2022
dc.description.abstract	The shortening rate of telomere length in blood cells is a potential predictive tool for health and lifespan. It is unknown whether the differences in telomere shortening observed between individuals are genetically determined or whether early environmental factors produce such differences. In this study, we analyzed the relative length of leukocyte telomeres throughout the first year of life in two animal colonies of the bovine and porcine species. The animals were generated by artificial insemination (AI) (control group) or by in vitro production (IVP) and transfer of embryos, either using conventional culture media (C-IVP, with bovine serum albumin as the only protein source) or enriched culture media (ET-IVP, with reproductive fluids as an extra source of proteins). Blood samples obtained 3 days, one month (day 28-30), 4 months (day 120), and one year (day 365) after birth were used. Cellular DNA was extracted, purified, and quantified following standardized protocols. Subsequently, the telomere length was determined by real-time quantitative PCR, normalizing the telomere amplification values with respect to the amount of the constitutive gene Rn18S (relative quantification). Statistical analysis (one way ANOVA and Bonferroni in cattle, two-sample t-test for inhomogeneous variances in pig, Pearson correlation and regression analysis) of the relative telomere size with respect to age and animal group allowed us to observe the dynamics of shortening that occurs in both species. The results showed a progressive shortening in telomere length with age, with the greatest shortening occurring in cattle at 30 days and in pigs at 120 days. As for the speed of shortening, in cattle, it was observed that the C-IVP group suffered a greater shortening between days 30 and 120 compared to the AI and ET-IVP groups. In pigs, a greater reduction was also observed in the C-IVP group than in the ET-IVP group. The results in both species suggest that supplementation with reproductive fluids during IVP reduces the rate of shortening of telomere length during the early development of individuals.	es
dc.format	application/pdf	es
dc.format.extent	2	es
dc.identifier.citation	Journal of Physiology and Biochemistry 2022, 78: pp O4-07
dc.identifier.doi	https://doi.org/10.1007/s13105-022-00923-3
dc.identifier.eissn	1877-8755
dc.identifier.issn	1138-7548
dc.identifier.uri	http://hdl.handle.net/10201/158201
dc.language	eng	es
dc.publisher	Springer Nature	es
dc.relation	PID2020-113366RB-I00 funded by MCIN/AEI/10.13039/501100011033/ and “FEDER Una manera de hacer Europa	es
dc.relation.ispartof	XL Congress of the Spanish Society of Physiological Sciences (SECF)	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s13105-022-00923-3	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Telomere length	es
dc.subject	Pig	es
dc.subject	Cow	es
dc.subject	Reproductive fluids	es
dc.subject.other	CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos	es
dc.title	Reproductive fluids reduce the rate of telomer shortening during the first year of life in in vitro derived- pig and cattle	es
dc.type	info:eu-repo/semantics/other	es
dspace.entity.type	Publication	es