Browsing by Subject "Cow"

Now showing 1 - 6 of 6
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    Influence of BMP-2 on early follicular development and mRNA expression of oocyte specific genes in bovine preantral follicles cultured in vitro
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Ross, Rodrigo O.D.S.; da Cunha, Ellen V.; Portela, Antonia M.L.R.; Passos, José R.S.; Costa, José J.N.; Silva, Anderson W.B.; Saraiva, Márcia V.A.; Peixoto, Christina A.; Donato, Mariana A.M.; van den Hur, Robert k; Silva, José R.V.
    This study evaluates the effect of different concentrations (0, 10, 50 and 100ng/mL) of bone morphogenetic protein-2 (BMP-2) on primordial and secondary follicle development. It also investigates the effects of FSH and BMP-2 on the growth, morphology, ultrastructure and expression of mRNA for GDF9, NLRP5 and NPM2 genes in secondary follicles cultured for 18 days. The presence of BMP-2 at all tested concentrations increased the development of primordial follicles in vitro, but the highest concentration of BMP-2 (100 ng/mL) reduced the percentage of normal follicles when compared with tissues cultured with 10 ng/mL BMP-2. During culture of secondary follicles, in contrast to higher concentrations (50 or 100 ng/mL), 10 ng/mL BMP-2 kept the morphology of follicles during initial stages of in vitro culture. This concentration of BMP-2 also benefits maintenance of the ultrastructure of 18-day cultured follicles. The presence of both BMP-2 and FSH in culture medium resulted in a significant (P<0.05) increase in follicular diameter after 18 days of culture. However, both FSH and BMP-2 reduced follicular mRNA expression of GDF9 and NLRP5 when compared to follicles cultured in media containing only FSH. In combination with FSH, BMP-2 reduced the mRNA levels of NPM2, when compared to follicles cultured in control medium. It is concluded from these data that 10 ng/mL BMP-2 promotes the growth of primordial in vitro and it helps to maintain the ultrastructure of secondary follicles, while FSH is more important for better expression of follicular markers like GDF9 and NLRP5.
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    Melatonin-like immunoreactivity in the pineal gland of the cow: an immunohistochemical study
    (Murcia : F. Hernández, 2004) Carvajal, J.C.; Gómez Esteban, M.B.; Carbajo, S.; Muñoz Barragan, L.
    With a view to checking the presence of melatonin in the pineal gland of the cow, in the present work we used six adult animals, ranging in age from one to six years, which were sacrificed at dawn. Sections of 6 µm thickness of Bouin-fixed and paraffin-embedded pineal glands were incubated in an anti-melatonin serum, which was provided by the Institute for Molecular and Cellular Recognition, Gunma University, Maebshi, Japan. After incubation and successive washings in PBS, some of the sections were treated with the avidin-biotinperoxidase complex (ABC) technique using antisera from Sigma, and developed with the method of Graham and Karnovsky (which employs 3,3’-diaminobenzidine and H2O2 as developer). Other sections were incubated in a goat-anti-rabbit IgG (H+L) bound to fluorochrome Cy5 for immunofluorescence studies. An intense reaction for melatonin was observed in the cytoplasm but not in the nucleus of melatonin secreting pinealocytes located in peripheral and intermediate zones of the pineal gland. Immunoabsorption of the antimelatonin primary antibody with melatonin at a dilution of 10 mM per 0.1 ml of serum prevented the reaction, as happened when any of the antisera used in the procedure were used. Immunoabsorption of antimelatonin serum with different amounts of bovine albumin (ranging between 1/5 to 1/50) failed to inhibit the immunoreactivity. When a bovine anti-albumin antibody was employed, working with the above methods, no immunoreaction was detected. Our data suggest that the pinealocytes of cows sacrificed at dawn contain immunoreactive melatonin.
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    Protocolo de sincronización de celo en vacas de lidia con monta natural
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2011) Mas, A.; Sanes, J.M.; Diego, R.; Vallejo, P.; Martínez Gomariz, Francisco; Seva Alcaraz, Juan; Anatomía y Anatomía Patológica Comparada
    La reproducción en la ganadería de lidia se encuentra condicionada por una serie de factores que pueden afectar tanto al semental como a las vacas de vientre, entre los que se pueden destacar el estrés derivado del manejo, la sanidad, el hábitat y la alimentación, así como la selección a la que han sido sometidos los progenitores. Es por esto que diferentes técnicas reproductivas que se aplican en otros bovinos no dan buenos resultados y provocan que el ganadero de bravo descarte su utilización. El objetivo de este estudio es obtener un protocolo de sincronización del celo válido para una ganadería de lidia con monta natural, para planificar la paridera. El estudio que llevamos a cabo se realizó en 20 vacas de la raza de lidia de diferentes edades y 1 semental de 8 años ya utilizado con anterioridad, que formaban un lote de reproductores de una explotación de lidia de la zona centro. Se empleó con las vacas un protocolo de sincronización de celo en dos manejos. Inicialmente se utiliza una esponja intravaginal de progestágeno y a los 8 días se inyecta por vía intramuscular gonadotropina coriónica equina y prostaglandina F2α, introduciendo entonces al animal sincronizado al cercado con el semental para monta natural. La sincronización y posterior introducción al cercado de monta se realizó de modo gradual con 4 vacas en cada grupo de sincronización. Los resultados obtenidos fueron positivos, con un incremento de la fertilidad en el conjunto de animales sincronizados hasta un 85%.
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    Rendimiento de OPU en vacas de raza murciano-levantina: respuesta a la estimulación ovárica mediante FSHp (Pluset®)
    (2025-04) Metony, Océane; Heras García, Sonia; Ramal Barea, Ismael; Ruiz López, Salvador; Romero Aguirregomezcorta, Jon; Departamentos; Universidad de Murcia, Servicio de publicaciones.; Facultades,Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria
    La técnica OPU (Ovum Pick-Up) permite obtener ovocitos de animales vivos para la fecundación (FIV) y producción in vitro de embriones (PIV). El objetivo del trabajo ha sido evaluar el rendimiento de la OPU en vacas de raza Murciano-Levantina (M-L), con el fin de recuperar ovocitos para la preservación de esta raza en peligro de extinción. Se seleccionaron 2 vacas M-L, Madroña y Unicornio, de 13 y 6 años, respectivamente, que fueron someti-das a un protocolo de estimulación hormonal con FSHp (Pluset®) durante 4 semanas, con un total de 4 sesiones de OPU/animal. Los ovocitos recuperados fueron cuantificados y clasificados en 5 grados (I a IV y degenera-dos) según su calidad ovocitaria. Los ovocitos viables (grados I a III) fueron vitrificados o madurados in vitro para posterior FIV y PIV. Se obtuvieron un total de 108 ovocitos, 34,26% de Madroña y 65,74% de Unicornio, de los cuales 92 (85,18%) fueron viables. El número medio de ovocitos totales (p = 0,03) y viables (p = 0,02) fue superior en Unicornio comparado con Madroña. Se obtuvieron significativamente más ovocitos grados I (p = 0,04) y III (p = 0,03) de Unicornio que de Madroña, y no se encontraron diferencias significativas para ovocitos grado II, IV y degenerados. En Madroña, el mayor número de ovocitos obtenidos fue grado III, con diferencias significativas (p = 0,03) entre este grupo y ovocitos grado I, IV y degenerados. En Unicornio, el mayor número de ovocitos fue grado III, con diferencias significativas (p = 0,02; p = 0,03), entre éste y ovocitos grado IV y degenerados. Este estudio demuestra que la OPU puede aplicarse con éxito en vacas M-L. Los resultados obtenidos muestran que existe variabilidad entre donantes en el número y calidad de ovocitos recuperados. Resultaría conveniente optimizar los protocolos de conservación genética en esta raza.
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    Reproductive fluids reduce the rate of telomer shortening during the first year of life in in vitro derived- pig and cattle
    (Springer Nature, 2022) Garcia-Cobarro, Claudia; Romar, Raquel; Ramis Vidal, Guillermo; Canovas, Sebastian; Gutiérrez-Adán, Alfonso; Coy, Pilar; Fisiología
    The shortening rate of telomere length in blood cells is a potential predictive tool for health and lifespan. It is unknown whether the differences in telomere shortening observed between individuals are genetically determined or whether early environmental factors produce such differences. In this study, we analyzed the relative length of leukocyte telomeres throughout the first year of life in two animal colonies of the bovine and porcine species. The animals were generated by artificial insemination (AI) (control group) or by in vitro production (IVP) and transfer of embryos, either using conventional culture media (C-IVP, with bovine serum albumin as the only protein source) or enriched culture media (ET-IVP, with reproductive fluids as an extra source of proteins). Blood samples obtained 3 days, one month (day 28-30), 4 months (day 120), and one year (day 365) after birth were used. Cellular DNA was extracted, purified, and quantified following standardized protocols. Subsequently, the telomere length was determined by real-time quantitative PCR, normalizing the telomere amplification values with respect to the amount of the constitutive gene Rn18S (relative quantification). Statistical analysis (one way ANOVA and Bonferroni in cattle, two-sample t-test for inhomogeneous variances in pig, Pearson correlation and regression analysis) of the relative telomere size with respect to age and animal group allowed us to observe the dynamics of shortening that occurs in both species. The results showed a progressive shortening in telomere length with age, with the greatest shortening occurring in cattle at 30 days and in pigs at 120 days. As for the speed of shortening, in cattle, it was observed that the C-IVP group suffered a greater shortening between days 30 and 120 compared to the AI and ET-IVP groups. In pigs, a greater reduction was also observed in the C-IVP group than in the ET-IVP group. The results in both species suggest that supplementation with reproductive fluids during IVP reduces the rate of shortening of telomere length during the early development of individuals.
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    The Witches of Hegel. On the Milky Origin of Evil
    (Universidad de Murcia. Servicio de Publicaciones, 2017) Rodríguez Gómez, Federico
    This essay proposes a reading of the philosophical problem of evil in Derrida’s works by calling attention to the figures of witch, cow and milk. I will follow this problem through his interpretations of Hegel, Nietzsche and Freud (especially as found in Glas [1974] and the unpublished seminar Manger l’autre [1989-90]). I first propose certain philosophical and psychoanalytic variations that aid in understanding a set of complicated cross- references directly or indirectly established with Hamacher’s works and the problem of eating (especially: Pleroma [1978]). Secondly, I show how the discussion of the problem of evil in these three figures can become a central locus for “deconstructive readings” (for readings that privilege a certain irreducible “complication” or “double-bind”).