Publication: The thyroid gland of Callithrix jacchus in organ culture
Authors
Maile, S. ; Merker, H. J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Thyroid glands from 7 marmosets (Callithrix
jacchus) of different age groups (newbom, 2 weeks, 2,7,
8 and 11 months, and 8 years) were grown as organ
culture according to Trowell at the mediumlair
interphase for 3 to 30 days. The morphology of thyroid
tissue was well preserved until the end of the culture
period. Necroses were only occasionally seen in
connective tissue. In contrast to the in vivo situation, the
number of lysosome-like inclusions changed
considerably and cell-cell contacts became loose. After
the addition of TSH the number of apical microvilli,
apical granules and coated vesicles rose. The basa1
surface of follicle cells enlarged due to the formation of
processes or ridges and invaginations. Mitosis could be
observed. These findings show that, using the Trowell
culture technique, thyroid tissue can be grown for up to
4 weeks without showing distinct changes in its
morphology. Its responsiveness to the application of
TSH is maintained in vitro. Hence, this technique is
suitable also for long-term endocrinological,
pharmacological and toxicological investigations.
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