Publication: Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics
Technique, SWATH-MS
Authors
Hernández-Caravaca, Ivan ; González-Brusi, Leopoldo ; Romero de Ávila, Mª José ; De Paco Matallana, Catalina ; Castaño-Molina, Mª Angeles ; Díaz-Meca, Lucía ; Sánchez-Romero, Javier ; Martínez-Alarcón, Laura ; Izquierdo Rico, María José ; Avilés Sánchez, Manuel ; Moros Nicolás, Carla ; Pelegrín Vivancos, Pablo
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DOI
10.3390/children10081423.
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info:eu-repo/semantics/article
Description
©2023. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/
This document is the Published, version of a Published Work that appeared in final form inChildren. To access the final edited and published work see https://doi.org/10.3390/children10081423
Abstract
Colostrum performs nutritional, anti-inflammatory and anti-infective functions and promotes immune system formation and organ development. The new coronavirus, SARS-CoV-2, has
generated concerns about viral transmission through human milk, with a lack of evidence about
human milk’s protective effects against the infection. This study aimed at analyzing presence of the
virus and at identifying the protein expression profile of human colostrum in active and COVID-19-
recovered patients. Colostrum samples were collected from women with COVID-19 (n = 3), women
recently recovered from the infection (n = 4), and non-infected women (n = 5). The samples were analyzed by means of RT-qPCR to determine presence of the virus and using SWATH-MS for proteomic
analysis. Proteomic results were then analyzed using bioinformatic methods. The viral tests were
negative for SARS-CoV-2 in the colostrum from COVID-19 patients. The proteomic analysis identified
301 common proteins in all samples analyzed. Nineteen proteins were upregulated and 7 were
downregulated in the COVID-19 group versus the control samples, whereas 18 were upregulated
and 7 were downregulated when comparing the COVID-19 group to the recovered group. Eleven
proteins were biomarkers of active COVID-19 infection. Ten were upregulated: ACTN1, CD36,
FAM3B, GPRC5B, IGHA2, IGK, PLTP, RAC1, SDCBP and SERPINF1, and one was downregulated:
PSAP. These proteins are mainly related to immunity, inflammatory response and protein transport.
In conclusion, the results of this study suggest that colostrum is not a vehicle for mother-to-child
SARS-CoV-2 transmission. Moreover, the colostrum’s proteome of active and recuperated patients
indicate that it could provide immune benefits to infants.
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Children
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by/4.0/