Publication:
Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

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Oral Diseases - 2020 - Camacho‐Alonso - Regeneration of lingual musculature in rats using myoblasts over porcine bladder.2021 (27).pdf(1.49 MB)
Date
2021
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Authors
Camacho Alonso, Fabio ; García Carrillo, Nuria ; Peñarrocha Oltra, David ; Peñarrocha Diago, Miguel ; Lacal Luján, José ; Buendía Marín, Antonio Julián ; Navarro Cámara, José Antonio
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Publisher
Wiley
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Dermatología, Estomatología, Radiología y Medicina Física
DOI
https://doi.org/10.1111/odi.13674
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info:eu-repo/semantics/article
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Abstract
Objectives: To use tissue engineering muscle repair (TEMR) for regenerating the lingual musculature of hemiglossectomized rats using neonatal myoblasts (NM) on porcine acellular urinary bladder matrix (AUBM). Material and Methods: The study used 80 male rats. A volumetric muscle loss (VML) injury was created on the left side of the tongue. The rats were randomized into four groups: Group 1 (AUBM + myoblasts); Group 2 (AUBM); Group 3 (myoblasts); and Group 4 (control). NM were obtained from neonatal rats. The animals were weighed on day 0 and just before euthanasia. Five rats in each group were euthanized at days 2, 14, 28, and 42; the tongues were prepared for morphometric analysis, postoperative left hemitongue weight, and immunohistochemical analysis (desmin, CD-31, and anti-neurofilament). Results: The weight gain from greatest to least was as follows: AUBM + myoblasts > myoblasts > AUBM > control. The tongue dorsum occupied by VML, and difference in mg between control side and intervened side from least to great was as follows: AUBM + myoblasts < myoblasts < AUBM < control. The order from highest to lowest antibody positivity was as follows: AUBM + myoblasts > myoblasts > AUBM > control. Conclusion: The use of porcine AUBM and NM for the regeneration of lingual musculature was found to be an effective TEMR treatment for repairing tongue VML injury.
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Hemiglossectomized tongues , Neonatal myoblasts , Porcine acellular urinary bladder matrix , Tissue engineering muscle repair , Volumetric muscle loss
Citation
Oral Diseases 2021 27(6):1580-1589
URI
http://hdl.handle.net/10201/138324
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