Navarro Cámara, José Antonio

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Navarro Cámara, José Antonio

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Navarro Cámara, José Antonio

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Universidad de Murcia. Departamento de Anatomía y Anatomía PatológicaComparadas

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Canine carcinosarcomas in the head
(SAGE Publications, 2005-11) Sánchez, J.; Villafranca, M.; Velarde, R.; Altamira, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Four cases of neoplasms in the heads of old dogs were studied. All the dogs showed both carcinomatous and sarcomatous malignant components in an admixed growing pattern. Histologic analysis of the tumors showed that the carcinomatous cells resembled squamous cell carcinoma in all dogs except one, where an adenoid arrangement of the neoplastic cells was also observed. The sarcomatous component showed osteoid matrix produced by pleomorphic poorly differentiated cells, which is regarded as a typical feature of osteosarcomas. Immunohistochemical analysis demonstrated that the components were positive only for cytokeratin (carcinomatous component) or vimentin (sarcomatous component). This observation led us to classify the neoplasms as true carcinosarcomas, thus providing evidence of a new preferential location for this unusual tumor in dog.
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Bone union formation in the rat mandibular symphysis using hydroxyapatite with or without simvastatin: effects on healthy, diabetic, and osteoporotic rats
(Springer Heidelberg, 2020-01-11) Camacho Alonso, Fabio; Martínez Ortiz, C.; Plazas Buendía, L.; Mercado Díaz, A.M.; Vilaplana Vivo, C.; Merino, J.J:; Martínez Beneyto, Yolanda; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Dermatología, Estomatología, Radiología y Medicina Física
Objective The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporotic rats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV). Materials and methods A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eightwithHA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). Results In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA+ SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone. Conclusions Combined application ofHAand SVimproves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporotic rats. Clinical relevance This studymight help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done.
Open Access
Value of anatomopathological examination in goats with a positive comparative intradermal tuberculin test as part of a tuberculosis control programme
(Wiley, 2025-01-23) Navarro Cámara, José Antonio; Sánchez Campillo, Joaquín; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparada; Facultades de la UMU::Facultad de Veterinaria
Background: Caprine tuberculosis is a chronic infectious disease caused by Mycobacterium bovis and Mycobacterium caprae. A tuberculosis control programme has been implemented using the comparative intradermal tuberculin (CIT) test. However, infection with Corynebacterium pseudotuberculsis and infection with or vaccination against Mycobacterium avium subspecies paratuberculosis (MAP)may have a negative impact on specificity. Therefore, a postmortem examination is necessary to verify the tuberculosis status of each animal where positive predictive value is low. Methods: Thirty-seven CIT-positive goats from 14 herds with a previously tuberculosis-free status were slaughtered between 2015 and 2020, and postmortem examination was carried out at the School of Veterinary Medicine of Murcia. Results: Anatomopathological examination ruled out the presence of tuberculosis lesions. In 23 of the goats, the anatomopathological examination revealed the presence of the paucibacillary form of paratuberculosis (associated with MAP infection), and in another six goats, Corynebacterium pseudotuberculosis infection was confirmed. Positive reactors have not reappeared in any of the herds in the annual CIT tests up to the year 2023. Limitations: Only those herds that sent their positive animals to the veterinary school were included in the study. Participation in the postmortem analysis was notmandatory for farmers. Conclusions: Anatomopathological examination is a useful, rapid and economically viable tool to determine the tuberculosis status of goat herds.
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Natural killer (NK) cells play a critical role in the early innate immune response to Chlamydophila abortus infection in mice
(Elsevier, 2004-01) Río, L. del; Caro, M. R.; Gallego, M. C.; Sánchez, J.; Cuello, F.; Salinas, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Ortega Hernández, Nieves; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Chlamydophila abortus, the aetiological agent of ovine enzootic abortion, induces a strong inflammatory reaction that leads to the T helper cell (Th1) specific immune response necessary for the clearance of infection. Because the role of natural killer (NK) cells during the first stages of this response has received little attention, this study focused on determining the function of these cells in a mouse model of infection. The location of NK cells in the liver and spleen of infected mice was examined immunohistochemically with an anti-Ly49G monoclonal antibody. The number of NK cells increased during the infection both in spleen and liver. In subsequent experiments, an anti-asialo GM1 polyclonal antibody was injected to deplete the NK cells. NK-depleted mice showed a substantial increase in their susceptibility to C. abortus infection, with high mortality rates and an increased burden of bacteria in the liver. Histopathological studies showed that inflammatory foci, composed mainly of neutrophils, were greater in size and number in depleted mice, while numerous chlamydial inclusions were associated with the foci. Serum concentrations of IFN-gamma, a key cytokine in the control of C. abortus infection, were substantially reduced in the NK-depleted mice. To establish the relationship between NK cells and other components of the innate immune response, neutrophils were depleted with the RB6-8C5 antibody. These cells were shown to be crucial in the recruitment of NK cells to the inflammatory foci.
Open Access
Histopathological and immunophenotypical characterization of a combined melanoma and mucoepidermoid carcinoma in a dog
(Murcia, Universidad de Murcia, Servicio de Publicaciones, 2009) Martínez, M. C.; Sánchez, J.; Vilafranca, M.; Altimira, J.; García, B.; Buendía Marín, Antonio Julián; Navarro Cámara, José Antonio; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
El presente trabajo describe el primer caso de un carcinoma mucoepidermoide canino pobremente diferenciado en el que se ha observado una proliferación maligna de melanocitos. La masa tumoral se localizó a nivel del 7º cuerpo vertebral en perro de raza mestiza. No se detectaron otros signos macroscópicos de carcinoma y/o melanoma oral o epitelial. El examen microscópico reveló la proliferación conjunta de ambas subpoblaciones. La proliferación y colonización de melanocitos en tumores de origen no melánico es rara y no se conoce la causa. En Patología humana, se han descrito casos de carcinomas mucoepidermoides pigmentados, pero en todos los casos se consideró la proliferación melanocítica como hiperplasia, no observándose en ningún caso signos de neoplasia. No existen en Patología humana ni veterinaria ningún caso descrito de carcinoma mucoepidermoide concomitante con un melanoma maligno, siendo éste el primer caso descrito.
Open Access
Estudio de las células productoras de prolactina (PRL)en cabritos (capra hircus)mediante técnicas de microscopía óptica y electrónica
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1997) Gómez Sánchez, Miguel Ángel; Gómez Cabrera, Serafín; Bernabé Salazar, Antonio; Navarro Cámara, José Antonio; Sánchez Campillo, Joaquín; Seva Alcaraz, Juan; Facultad de Veterinaria
Open Access
Sistema inmunitario asociado al intestino en la cabra: distribución y evolución de las poblaciones linfocitarias
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1999) Pallarés Martínez, Francisco José; Gómez Sánchez, Miguel Ángel; Bernabé Salazar, Antonio; Navarro Cámara, José Antonio; Seva Alcaraz, Juan; Facultad de Veterinaria
Open Access
Modificaciones ultraestructurales d elas células foliculoestrelladas y cavidades foliculares adenohipofisarias en cabras (Capra hircus) en anestro, gestación y producción láctea
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1988) Gómez Sánchez, Miguel Ángel; Bernabé Salazar, Antonio; Gómez Cabrera, Serafín; Sánchez Campillo, Joaquín; Carrasco, L.; Navarro Cámara, José Antonio; Facultad de Veterinaria
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Effects of diabetes on oxidative stress, periodontal ligament fiber orientation and matrix metalloproteinase 8 and 9 expression during orthodontic tooth movement
(2021) Vicente, Ascensión; Bravo-González, Luis-Alberto; Camacho-Alonso, Fabio; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Dermatología, Estomatología, Radiología y Medicina Física
Objectives: To evaluate the influence of diabetes on oxidative stress, periodontal ligament (PDL) orientation, and matrix metalloproteinase (MMP) 8 and 9 expressions during orthodontic tooth movement in a rat model. Materials and methods: An orthodontic appliance was placed in 60 Sprague-Dawley rats divided into three groups: normoglycemics (n = 20) and two streptozotocin-induced diabetic groups, one untreated (n = 20) and one insulin-treated (n =20). At 24, 48, and 72 h and 1 week, rats were sacrificed. At each time point, myeloperoxidase (MPO) and malondialdehyde (MDA) were quantified by spectrophotometry, tooth movement was evaluated by micro-CT analysis, and hematoxylin and eosin staining was used to evaluate PDL fiber orientation and immunohistochemistry staining with semi-quantitative H-score analysis of MMP-8 and MMP-9 was performed. Results: At 24 h, MPO activity was significantly higher in untreated-diabetics than normoglycemics. At 24 and 48 h, the MDA level in untreated-diabetic rats was significantly higher than in normoglycemics and insulin-treated animals. At 72 h and 1 week, PDL fibers were oriented significantly more irregularly in untreated-diabetics than in normoglycemics. At all time points,MMP-8 and MMP-9 expressions were significantly higher in both diabetic groups than in the normoglycemic group. After the second day, tooth movement was significantly greater in untreated-diabetics than in the insulin-treated and normoglycemic groups. Conclusions: Mechanical stress in untreated-diabetic rats produces more inflammatory response, oxidative stress, tooth movement, PDL disorganization, and MMP-8 and MMP-9 expressions than among normoglycemics. Insulin reverses these effects, favoring the reorganization of periodontal ligament. Clinical relevance: Our results suggest that the application of orthodontic force in diabetic patients would increase inflammation and delay periodontal restructuring. Insulin would partly reverse this situation although glycemic decompensation episodes may occur. For these reasons, the periods between fixed orthodontic appliance activations should be of sufficient duration to allow adequate tissue recovery.
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Characterization of a murine model of intranasal infection suitable for testing vaccines against C. abortus
(Elsevier, 2007-01-15) Nicolás, L.; Gallego, M. C.; Sánchez, J.; Caro, M. R.; Salinas, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Ortega Hernández, Nieves; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Mouse models have been widely used to test candidate vaccines against Chlamydophila abortus infection in mice. Although the induction of a systemic infection by endogenous or intraperitoneal inoculation is a useful tool for understanding the immune mechanism involved in the protection conferred by the vaccination, a different approach is necessary to understand other factors of the infection, such as mucosal immunity or the colonization of target organs. To test whether C. abortus intranasal model of infection in mice is a useful tool for testing vaccines in a first group of experiments mice, were infected intranasally with C. abortus to characterize the model of infection. When this model was used to test vaccines, two inactivated experimental vaccines, one of them adjuvated with QS-21 and another with aluminium hydroxide, and a live attenuated vaccine (strain 1B) were used. Non-vaccinated control mice died within the first 8 days, after displaying substantial loss of weight. Histologically, the mice showed lobar fibrinopurulent bronchointerstitial pneumonia. Prior immunization with QS-21 adjuvated vaccine or 1B vaccine presented mortality and the recipients showed a greater number of T cells in the lesions, especially CD8+ T cells, than the control mice and mice immunized with vaccine adjuvated with aluminium hydroxide. The results confirm that the C. abortus intranasal model of infection in mice is a useful tool for testing vaccines