Publication: Effects of alcohol on laminin in rat gastric mucosa
Authors
Rightor, Kathryn S. ; Schmidt, Carmen L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Following ethanol exposure, tlie gastric
surface epithelium often exfoliates. leaving a denuded
basal lamina. Viable cells from tlie gland migrate along
thc basal lamina to repair the defect. a process known
as restitution. Laniinin. the major lion-collagenous
glycoprotein of basal laminac, functions in cellular
adhesion and migration and. tliereforc. any alteration
of this molecule b! ethunol may influence subsequent
rcs t i t~~t ionA. fter a 5 o r 60 minutes treatment with
saline. 5OCl/ll or 100% ethanol. gastric tissues were
removed from fasted female Sprag~~e-Dawlerayt s, fixed
in 1% paraformaldehyde and processed in Lowicryl.
Once embedded and sectioned. specimens were incubated
in anti-laminin followed hy protein A-gold. The area
of lamina drnsa from interfoveolal-. pit and gland regions
was measured and g,old particles counted. Absolute
cthanol caused dirnin~shed imrnunogold binding in all
regions at all time periods. except the gland at 60
minutes. Exposure to 50% ethanol for 5 minutes did not
alter laminin bincling. although 60 minutes after 50%)
alcohol, laminin immunolabelling was increased.
Alcohol concentration alters laminin immuno~old
binding. and therefore may influence restitituion.
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