Publication: Morphologic changes within the cerebellar cortex in the unilateral 6-hydroxydopamine lesioned rat model for Parkinson disease
Authors
Wu, Chenghua ; Fan, Guoguang ; Wu, Chunli ; Yu, Guibo ; Li, Zixuan
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-760
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info:eu-repo/semantics/article
Description
Abstract
Parkinson’s disease (PD) is a common
neurodegenerative disorder caused by the progressive
loss of dopaminergic neurons in the substantia nigra.
Most investigations have focused on the cerebral regions
such as the basal ganglia, thalamus, or the substantia
nigra, but whether there is pathologic impairment within
the cerebellum has rarely been assessed. Synapsin and
neurofilament as the inner markers of neurons and
synapses reflect the functional state by their distribution
or expression. Significant morphologic changes at the
cellular level have been demonstrated directly or
indirectly in multiple neurodegenerative diseases. The
purpose of this study was to determine whether the
behavioral abnormalities that accompany PD are
associated with the cerebellum using an in vivo 6-
hydroxydopamine lesioned rat model. Forty-two rats
were divided into three groups, the Parkinsonian group
(N=22), sham group (N=10) and control group (N=10).
The dopaminergic lesion was determined by immunohistochemical analysis for tyrosine hydroxylaseimmunopositive cells. Immunohistochemical studies
showed that the density of synapsin I in the granular
layer of the cerebellum on both sides of the Parkinsonian
-model was not statistically significantly different
compared to the control and sham groups. However,
expression of neurofilament H in the cortex within
bilateral paramedian lobule (PML) and Crus 2 of the
ansiform lobule (C2AL) in cerebellum posterior lobe of
Parkinsonian rats was decreased compared with controls
(P<0.05), especially in the loss of Purkinje cells and the
presence of morphologic abnormalities in the cell
nucleus. The study suggested that loss of neurons and
synapses may take place in the cerebellar cortex of
Parkinson’s disease, and might play an important role in
the pathologic mechanism of PD.
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Citation
Histology and Histopathology, Vol.31, nº12, (2016)
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