Publication: Ploidy and chromatin pattern analysis as an aid for cervical smear diagnosis
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Date
2002
Authors
Nemec, E. ; Vandeputte, S. ; Van Pachterbeke, C. ; Vokaer, R. ; Budel, V. ; Deprez, C. ; Kiss, R. ; Decaestecker, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In the present study we used computerassisted
microscopy to analyze the morphology of
Feulgen-stained cell nuclei in cell populations obtained
at the same time as routinely performed cervical smears
and in the same way. We investigated in a series of 110
cases whether the quantitative morphonuclear
description of cytological cervical samples is able to aid
pathologists to distinguish between benign and more
suspect premalignant lesions. For this task nuclear DNA
content, nuclear morphometry (size and anisonucleosis
l evel) and chromatin pattern-related parameters were
compiled for each specimen enrolled in the database. A
set of 32 normal and 17 high-grade squamous
intraepithelial lesion (HSIL) specimens (with diagnostic
c o n firmations) were selected as references and used to
establish a discriminant model on the basis of cytometrygenerated
variables. This model was then used to score
the remaining 61 cases in our series (including cases
exhibiting benign cellular changes, squamous cells of
undetermined significance, low-grade SIL and cancers).
The results show that a model discriminating efficiently
between normal and HSIL groups can be obtained by
combining 5 quantitative features (1 DNA ploidyrelated,
2 morphometrical and 2 chromatin tex t u r e
features). A 97% specificity and an 88% sensitiv i t y
characterized the boundary so established. When applied
to new cases, the model was in fact able to correct
diagnoses for cases which had been down- or up-graded
on the basis of the Bethesda system, and provided scores
in accordance with histological control.
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