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Ploidy and chromatin pattern analysis as an aid for cervical smear diagnosis

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dc.contributor.authorNemec, E.es
dc.contributor.authorVandeputte, S.es
dc.contributor.authorVan Pachterbeke, C.
dc.contributor.authorVokaer, R.
dc.contributor.authorBudel, V.
dc.contributor.authorDeprez, C.
dc.contributor.authorKiss, R.
dc.contributor.authorDecaestecker, C.
dc.date.accessioned2011-05-11T10:58:17Z
dc.date.available2011-05-11T10:58:17Z
dc.date.issued2002
dc.description.abstractIn the present study we used computerassisted microscopy to analyze the morphology of Feulgen-stained cell nuclei in cell populations obtained at the same time as routinely performed cervical smears and in the same way. We investigated in a series of 110 cases whether the quantitative morphonuclear description of cytological cervical samples is able to aid pathologists to distinguish between benign and more suspect premalignant lesions. For this task nuclear DNA content, nuclear morphometry (size and anisonucleosis l evel) and chromatin pattern-related parameters were compiled for each specimen enrolled in the database. A set of 32 normal and 17 high-grade squamous intraepithelial lesion (HSIL) specimens (with diagnostic c o n firmations) were selected as references and used to establish a discriminant model on the basis of cytometrygenerated variables. This model was then used to score the remaining 61 cases in our series (including cases exhibiting benign cellular changes, squamous cells of undetermined significance, low-grade SIL and cancers). The results show that a model discriminating efficiently between normal and HSIL groups can be obtained by combining 5 quantitative features (1 DNA ploidyrelated, 2 morphometrical and 2 chromatin tex t u r e features). A 97% specificity and an 88% sensitiv i t y characterized the boundary so established. When applied to new cases, the model was in fact able to correct diagnoses for cases which had been down- or up-graded on the basis of the Bethesda system, and provided scores in accordance with histological control.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/20796
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCervical cytologyes
dc.subjectDysplasiaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titlePloidy and chromatin pattern analysis as an aid for cervical smear diagnosises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.17, nº 2 (2002)