Publication:
Comparison of retinal nerve fiber layer thinning and retinal ganglion cell loss after optic nerve transection in adult albino rats

Thumbnail Image
Files
Rovere (IOVS 2015) SD-OCT Retinal Thickness ONT Rat.pdf(2.33 MB)
Date
2015-07-15
relationships.isAuthorOfPublication
Profile Picture
Person
Profile Picture
Person
Profile Picture
Person
Profile Picture
Person
Profile Picture
Person
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Rovere, Giuseppe ; Nadal-Nicolás, Francisco Manuel ; Agudo Barriuso, Marta ; Sobrado Calvo, Paloma ; Nieto López, Leticia ; Nucci, Carlo ; Villegas Pérez, Maria Paz ; Vidal Sanz, Manuel
item.page.secondaryauthor
Facultades de la UMU::Facultad de Medicina
item.page.director
Publisher
Association for Research in Vision and Ophthalmology.
publication.page.editor
publication.page.department
Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
DOI
https://doi.org/10.1167/iovs.15-17145
item.page.type
info:eu-repo/semantics/article
Description
Abstract
PURPOSE. We compared the time-course and magnitude of retinal nerve fiber layer (RNFL) thinning with that of retinal ganglion cell (RGC) loss after intraorbital optic nerve transection (IONT) in adult rats. METHODS. At 3, 7, 12, or 21 days, or 1, 2, or 4 months after ONT, the retinas were imaged with spectral-domain optical coherence tomography (SD-OCT) using the circular-peripapillary scan and volume scan raster pattern (61 horizontal sections equally spaced) both centered in the optic nerve. In all sections, the RNFL and retinal thickness were measured to obtain the total values of the peripapillary scan and the values of three concentric sectors (400, 1200, and 2400 lm in diameter) from the volume scan. After SD-OCT, retinas were dissected and immunoreacted for Brn3a and neurofilaments (pNFH) to identify RGCs and their intraretinal axons, respectively. Total numbers of RGCs were quantified. RESULTS. Thinning of the RNFL was first observed at 12 days in peripapillary scan (10% decrease) and progressed up to 4 months (72% decrease). The volume scan showed transient RNFL swelling in central and medial sectors at 3, 7, and 12 days followed by progressive significant thinning first observed at 21 days (central sector, 30%; medial sector, 40%) and 12 days (peripheral sector, 15%), respectively. Following IONT, Brn3aþ RGCs decreased to approximately 80%, 52%, 17%, 9%, 5%, 3%, and 2% at 3, 7, 12, 21 days, and at 1, 2, and 4 months, respectively. Retinal ganglion cell axon immunodetection decreased from 12 days onwards. CONCLUSIONS. After IONT, RGC death is more severe and precedes thinning of the RNFL.
publication.page.subject
Retinal ganglion cells RGCs , Spectral domain optical coherence tomography SDOCT , Brn3a , Antibodies antineurofilaments , Intraorbital optic nerve transection IONT , Adult albino rats , Retinal nerve fiber layer , Retrograde axonal degeneration , Axotomy , Glaucoma , Retinal neurodegeneration
Citation
Invest Ophthalmol Vis Sci. 2015 Jul;56(8):4487-98.
URI
http://hdl.handle.net/10201/194589
item.page.embargo
Collections
Artículos
Ir a Estadísticas