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Comparison of retinal nerve fiber layer thinning and retinal ganglion cell loss after optic nerve transection in adult albino rats

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dc.contributor.authorRovere, Giuseppe
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorSobrado Calvo, Paloma
dc.contributor.authorNieto López, Leticia
dc.contributor.authorNucci, Carlo
dc.contributor.authorVillegas Pérez, Maria Paz
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T11:14:17Z
dc.date.available2026-01-27T11:14:17Z
dc.date.copyright© 2015 The Association for Research in Vision and Ophthalmology, Inc.
dc.date.issued2015-07-15
dc.description.abstractPURPOSE. We compared the time-course and magnitude of retinal nerve fiber layer (RNFL) thinning with that of retinal ganglion cell (RGC) loss after intraorbital optic nerve transection (IONT) in adult rats. METHODS. At 3, 7, 12, or 21 days, or 1, 2, or 4 months after ONT, the retinas were imaged with spectral-domain optical coherence tomography (SD-OCT) using the circular-peripapillary scan and volume scan raster pattern (61 horizontal sections equally spaced) both centered in the optic nerve. In all sections, the RNFL and retinal thickness were measured to obtain the total values of the peripapillary scan and the values of three concentric sectors (400, 1200, and 2400 lm in diameter) from the volume scan. After SD-OCT, retinas were dissected and immunoreacted for Brn3a and neurofilaments (pNFH) to identify RGCs and their intraretinal axons, respectively. Total numbers of RGCs were quantified. RESULTS. Thinning of the RNFL was first observed at 12 days in peripapillary scan (10% decrease) and progressed up to 4 months (72% decrease). The volume scan showed transient RNFL swelling in central and medial sectors at 3, 7, and 12 days followed by progressive significant thinning first observed at 21 days (central sector, 30%; medial sector, 40%) and 12 days (peripheral sector, 15%), respectively. Following IONT, Brn3aþ RGCs decreased to approximately 80%, 52%, 17%, 9%, 5%, 3%, and 2% at 3, 7, 12, 21 days, and at 1, 2, and 4 months, respectively. Retinal ganglion cell axon immunodetection decreased from 12 days onwards. CONCLUSIONS. After IONT, RGC death is more severe and precedes thinning of the RNFL.
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dc.identifier.citationInvest Ophthalmol Vis Sci. 2015 Jul;56(8):4487-98.
dc.identifier.doihttps://doi.org/10.1167/iovs.15-17145
dc.identifier.eissn1552-5783
dc.identifier.issn0146-0404
dc.identifier.urihttp://hdl.handle.net/10201/194589
dc.languageeng
dc.publisherAssociation for Research in Vision and Ophthalmology.
dc.relationSupported by Spanish Ministry of Economy and Competitiveness: SAF-2012-38328; Instituto de Salud Carlos III-FEDER ‘‘Una manera de hacer Europa’’ PI13/00643, PI13/01266, and Red Temática de Investigación Cooperativa en Oftalmología RETICS: RD12/0034/0014.
dc.relation.publisherversionhttps://iovs.arvojournals.org/article.aspx?articleid=2397687
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectRetinal ganglion cells RGCs
dc.subjectSpectral domain optical coherence tomography SDOCT
dc.subjectBrn3a
dc.subjectAntibodies antineurofilaments
dc.subjectIntraorbital optic nerve transection IONT
dc.subjectAdult albino rats
dc.subjectRetinal nerve fiber layer
dc.subjectRetrograde axonal degeneration
dc.subjectAxotomy
dc.subjectGlaucoma
dc.subjectRetinal neurodegeneration
dc.subject.odsObjetivo 3: Salud
dc.titleComparison of retinal nerve fiber layer thinning and retinal ganglion cell loss after optic nerve transection in adult albino rats
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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