Publication: The therapeutic effects of apigenin and dexamethasone on 5-fluorouracil-induced oral mucositis – a pilot study using a Syrian hamster model
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Date
2016-07-05
Authors
Molina Prats, Patricia ; Gómez García, Francisco ; Martínez Díaz, Francisco ; Amaral Mendes, Rui ; López Jornet, María Pia
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Publisher
Wiley
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DOI
https://doi.org/10.1111/jop.12473
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info:eu-repo/semantics/article
Description
© 2016 John Wiley & Son. This document is the Published version of a Published Work that appeared in final form in Journal of Oral Pathology & Medicine. To access the final edited and published work see https://doi.org/10.1111/jop.12473
Abstract
Objective: Oral mucositis (OM) is a common complication of chemotherapy and radiotherapy. The aim of this study was to evaluate the effects of treating 5-fluorouracil-induced OM with apigenin and dexamethasone.
Methods: Thirty-six male Syrian hamsters were randomly assigned to one of three groups: control (50% acetic acid + 5-FU), 50% acetic acid + 5-FU + potassium Apigenin (KA), and 50% acetic acid + 5-FU + dexamethasone. The animals from each group were sacrificed 5, 7, 10, and 14 days after inducing the mucositis, and two samples collected from each animal, accounting a total of 72 samples. Macroscopic changes were assessed by histomorphometric analysis, with ulcers being assessed by imaging analysis and the number of inflammatory cells in the ulcerated region quantified in all periods through histomorphometric analysis (H&E). Furthermore, immunohistochemical changes were evaluated by proliferating cell nuclear antigen. Results: All groups presented an increased inflammatory infiltrate after 7 days, compared to other evaluation times (P ≥ 0.05). There was significant difference between apigenin and control group in the 10-days period. Lower quantity of inflammatory cells in the apigenin-treated group in comparison with control group in the 7- and 10-days periods was observed (P < 0.05). No statistically significant difference was verified among the groups in 5- and 14-days periods. The healing process of the control group was slower than that of apigenin and dexamethasone-treated groups, with an overall significant difference between apigenin and the control group in the 10-days period. Conclusions: Apigenin treatment may enhance healing of OM induced by 5-fluorouracil, thus suggesting that more extensive research in this area may be useful to assess the role of agents of natural origin capable of preventing OM. Hence, further studies involving broader samples are need to confirm the therapeutic potential shown by this study.
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Citation
J Oral Pathol Med (2017) 46: 142–147
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