Browsing by Subject "Dexamethasone"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Open Access
    Period 1 (PER1): A novel glucocorticoid-responsive gene involved in cortisol-induced proliferation of androgen-independent human prostate cancer DU145 cells
    (2025) Hiroki Shimada; Atsushi Yokoyama; Yasuhiro Nakamura; Shuko Hata; Biología Celular e Histología
    Glucocorticoid receptor (GR) has been implicated in prostate carcinoma and possibly involved in cancer growth and progression. Therefore, in this study, we examined whether the genes involved in cell proliferation regulation were induced by cortisol in androgen-independent human prostate cancer DU145 cells. Gene expression profiling of the DU145 cell pathway was conducted using the RT2 Profile PCR Array System, quantitative reverse transcriptase polymerase chain reaction, and immunoblot analysis. These analyses demonstrated that the expression level of Period 1 (PER1), a gene associated with an organism's biological clock and involved in anti-apoptosis and cell growth, was markedly increased in DU145 cells treated with dexamethasone (DEX). In addition, analysis using short hairpin RNA demonstrated that products of PER1 were involved in the DEX-induced proliferation of DU145 cells. Therefore, PER1 is considered a glucocorticoid-responsive gene that regulates DU145 cell proliferation induced by GR stimulation, thus potentially playing an important role in GR-related androgen-independent human prostate cancer.
  • Thumbnail Image
    Publication
    Restricted
    The therapeutic effects of apigenin and dexamethasone on 5-fluorouracil-induced oral mucositis – a pilot study using a Syrian hamster model
    (Wiley, 2016-07-05) Molina Prats, Patricia; Gómez García, Francisco; Martínez Díaz, Francisco; Amaral Mendes, Rui; López Jornet, María Pia; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
    Objective: Oral mucositis (OM) is a common complication of chemotherapy and radiotherapy. The aim of this study was to evaluate the effects of treating 5-fluorouracil-induced OM with apigenin and dexamethasone. Methods: Thirty-six male Syrian hamsters were randomly assigned to one of three groups: control (50% acetic acid + 5-FU), 50% acetic acid + 5-FU + potassium Apigenin (KA), and 50% acetic acid + 5-FU + dexamethasone. The animals from each group were sacrificed 5, 7, 10, and 14 days after inducing the mucositis, and two samples collected from each animal, accounting a total of 72 samples. Macroscopic changes were assessed by histomorphometric analysis, with ulcers being assessed by imaging analysis and the number of inflammatory cells in the ulcerated region quantified in all periods through histomorphometric analysis (H&E). Furthermore, immunohistochemical changes were evaluated by proliferating cell nuclear antigen. Results: All groups presented an increased inflammatory infiltrate after 7 days, compared to other evaluation times (P ≥ 0.05). There was significant difference between apigenin and control group in the 10-days period. Lower quantity of inflammatory cells in the apigenin-treated group in comparison with control group in the 7- and 10-days periods was observed (P < 0.05). No statistically significant difference was verified among the groups in 5- and 14-days periods. The healing process of the control group was slower than that of apigenin and dexamethasone-treated groups, with an overall significant difference between apigenin and the control group in the 10-days period. Conclusions: Apigenin treatment may enhance healing of OM induced by 5-fluorouracil, thus suggesting that more extensive research in this area may be useful to assess the role of agents of natural origin capable of preventing OM. Hence, further studies involving broader samples are need to confirm the therapeutic potential shown by this study.