Publication: Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism
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Date
2020-02-22
Authors
Sánchez-Pujante, Pedro Joaquín ; Gionfriddo, Matteo ; Díaz-Vivancos, Pedro ; Almagro Romero, Lorena ; Pedreño García, María Ángeles ; Sabater Jara, Ana Belén
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.jplph.2020.153136
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info:eu-repo/semantics/article
Description
© 2020, Elsevier GnbH. Allrights reserved. This document is the Published version of a Published Work that appeared in final form in Journal of Plant Physiology. To access the final edited and published work see https://doi.org/10.1016/j.jplph.2020.153136
Abstract
Elicited broccoli suspension-cultured cells (SCC) provide a useful system for obtaining bioactive compounds, including glucosinolates (GS) and phenolic compounds (PCs). In this work, coronatine (Cor) and methyl jas
monate (MJ) were used to increase the bioactive compound production in broccoli SCC. Although the use of Cor and MJ in secondary metabolite production has already been described, information concerning how elicitors
affect cell metabolism is scarce. It has been suggested that Cor and MJ trigger defence reactions affecting the antioxidative metabolism. In the current study, the concentration of 0.5 μM Cor was the most effective treatment for increasing both the total antioxidant capacity (measured as ferulic acid equivalents) and glucosinolate content in broccoli SCC. The elicited broccoli SCC also showed higher polyphenol oxidase activity than the control cells. Elicitation altered the antioxidative metabolism of broccoli SCC, which displayed biochemical changes in antioxidant enzymes, a decrease in the glutathione redox state and an increase in lipid peroxidation
levels. Furthermore, we studied the effect of elicitation on the protein profile and observed an induction of defence-related proteins. All of these findings suggest that elicitation not only increases bioactive compound production, but it also leads to mild oxidative stress in broccoli SCC that could be an important factor triggering the production of these compounds.
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Citation
Journal of Plant Physiology 248 (2020) 153136
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