Browsing by Subject "Glucosinolates"

Now showing 1 - 7 of 7
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    Bioavalability and new biomarkers of cruciferous sprouts consumption
    (Elsevier, 2017-07-21) Baenas, Nieves; Suárez-Martínez, Clara; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    The evaluation of the bioavailability of cruciferous compounds is one of several challenges in the design of clinical trials. A 7-days-cross-over study with fourteen women was undertaken to compare the bioavailability of glucosinolates from broccoli and radish sprouts. The urinary excretion of isothiocyanates, indoles and metabolites was analysed by UHPLC-QqQ-MS/MS. Even though the bioavailability of broccoli compounds has been studied, as far as we are aware, there are not any biomarkers established for radish sprouts intake. For the first time, sulforaphene, sulforaphane-N-acetyl-L-cysteine (SFN-NAC) and 3,3’diindolylmethane (DIM), were studied as biomarkers of dietary exposure to radish. The SFN-NAC and DIM were already considered biomarkers of broccoli consumption. Higher excretion of conjugated isothiocyanates and homogeneous excretion of indoles were found during the first 12 h after ingestion. Metabolites were excreted homogeneously during the study, suggesting no accumulation. These results provide valuable information to better understand the bioavailability of cruciferous bioactives.
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    Biotic Elicitors Effectively Increase The Glucosinolates Content In Brassicaceae Sprouts
    (American Chemical Society, 2014-01-31) Baenas, Nieves; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    Several biotic elicitors have been used in Brassicaceae species to enhance their phytochemical quality. However, there is no comparison between elicitors under controlled-growth conditions. In order to draw general conclusions about the use of elicitors to enrich ready-to-eat sprouts in health-promoting glucosinolates, the aim of this study was to unvail the effect of the phytohormones methyl jasmonate (25µM), jasmonic acid (150 µM), and salicylic acid (100 µM), the oligosaccharides glucose (277mM) and sucrose (146mM), and the amino acid DL-methionine (5mM), as elicitors over 8-day sprouting Brassica oleraceae (broccoli), Brassica napus (rutabaga cabbage), Brassica rapa (turnip) and Raphanus sativus (China rose radish and red radish), representative species high in glucosinolates previously studied. Results indicated that the phytohormones methyl jasmonate and jasmonic acid, and the sugars, acted as effective elicitors, increasing the total glucosinolate contents and, particularly, health related compounds such as glucoraphanin, glucoraphenin, dehydroerucin and indoles, in all the Brassicaceae species studied.
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    Dietary-Fibre-Rich Fractions Isolated from Broccoli Stalks as a Potential Functional Ingredient with Phenolic Compounds and Glucosinolates
    (MDPI, 2022-11-01) Periago, María Jesús; Núñez-Gómez, Vanesa; Baenas, Nieves; Moreno, Diego; González-Barrio, Rocío
    The Brassica oleracea industry generates large amounts of by-products to which value could be added because of the characteristics of their composition. The aim was to extract different fibre fractions from broccoli stalks to obtain potential new added-value ingredients. Using an ethanol and water extraction procedure, two fibre-rich fractions (total fibre fraction, TFB, and insoluble fibre fraction, IFB) were obtained. These fractions were analysed to determine the nutritional, (poly)phenols and glucosinolates composition and physicochemical properties, comparing the results with those of freeze-dried broccoli stalks (DBS). Although TFB showed a higher content of total dietary fibre, IFB had the same content of insoluble dietary fibre as TFB (54%), better hydration properties, higher content of glucosinolates (100 mg/100 g d.w.) and (poly)phenols (74.7 mg/100 g d.w.). The prebiotic effect was evaluated in IFB and compared with DBS by in vitro fermentation with human faecal slurries. After 48 h, the short-chain fatty acid (SCFA) production was higher with IFB than with DBS because of the greater presence of both uronic acids, the main component of pectin, and (poly)phenols. These results reveal that novel fibre-rich ingredients—with antioxidant, technological and physiological effects—could be obtained from broccoli stalks by using green extraction methods.
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    Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism
    (Elsevier, 2020-02-22) Sánchez-Pujante, Pedro Joaquín; Gionfriddo, Matteo; Díaz-Vivancos, Pedro; Almagro Romero, Lorena; Pedreño García, María Ángeles; Sabater Jara, Ana Belén; Biología Vegetal
    Elicited broccoli suspension-cultured cells (SCC) provide a useful system for obtaining bioactive compounds, including glucosinolates (GS) and phenolic compounds (PCs). In this work, coronatine (Cor) and methyl jas monate (MJ) were used to increase the bioactive compound production in broccoli SCC. Although the use of Cor and MJ in secondary metabolite production has already been described, information concerning how elicitors affect cell metabolism is scarce. It has been suggested that Cor and MJ trigger defence reactions affecting the antioxidative metabolism. In the current study, the concentration of 0.5 μM Cor was the most effective treatment for increasing both the total antioxidant capacity (measured as ferulic acid equivalents) and glucosinolate content in broccoli SCC. The elicited broccoli SCC also showed higher polyphenol oxidase activity than the control cells. Elicitation altered the antioxidative metabolism of broccoli SCC, which displayed biochemical changes in antioxidant enzymes, a decrease in the glutathione redox state and an increase in lipid peroxidation levels. Furthermore, we studied the effect of elicitation on the protein profile and observed an induction of defence-related proteins. All of these findings suggest that elicitation not only increases bioactive compound production, but it also leads to mild oxidative stress in broccoli SCC that could be an important factor triggering the production of these compounds.
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    In vitro study of the differential anti-inflammatory activity of dietary phytochemicals upon human macrophage-like cells as a previous step for dietary intervention
    (MDPI, 2024-10-05) Ruiz Alcaraz, Antonio José; Baquero, Lorena; Martínez Pérez-Munar, Paula; Oliva-Bolarín, Alba; Sánchez-Martínez, María A.; Ramos-Molina, Bruno; Núñez-Sánchez, María A.; Moreno, Diego A.; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
    Chronic inflammatory diseases pose a substantial health challenge globally, significantly contributing to morbidity and mortality. Addressing this issue requires the use of effective anti-inflammatory strategies with fewer side effects than those provoked by currently used drugs. In this study, a range of phytochemicals (phenolic di-caffeoylquinic acid (Di-CQA), flavonoid cyanidin-3,5-diglucoside (Cy3,5DiG), aromatic isothiocyanate sinalbin (SNB) and aliphatic isothiocyanate sulforaphane (SFN)) sourced from vegetables and fruits underwent assessment for their potential anti-inflammatory activity. An in vitro model of human macrophage-like cells treated with a low dose of LPS to obtain a low degree of inflammation that emulates a chronic inflammation scenario revealed promising results. Cell viability and production of the key pro-inflammatory cytokines were assessed in the presence of various phytochemicals. The compounds Di-CQA and Cy-3,5-DiG, within low physiologically relevant doses, demonstrated notable anti-inflammatory effects by significantly reducing the production of key pro-inflammatory cytokines TNF-α and IL-6 without affecting cell viability. These findings underscore the potential of plant-derived bioactive compounds as valuable contributors to the prevention or treatment of chronic inflammatory diseases. These results suggest that these compounds, whether used individually or as part of natural mixtures, hold promise for their inclusion in nutritional interventions designed to mitigate inflammation in associated pathologies.
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    Optimizing elicitation and seed priming to enrich broccoli and radish sprouts in glucosinolates
    (Elsevier, 2016-02-26) Baenas, Nieves; Villaño, Débora; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    Elicitation is a cheaper and socially acceptable tool for improving plant food functionality. Our objective was to optimize the treatment doses of the elicitors: methyl jasmonate (MeJA), jasmonic acid (JA) and DL-methionine (MET), in order to find a successful and feasible treatment to produce broccoli and radish sprouts with enhanced levels of health-promoting glucosinolates. Also a priming of seeds as a novel strategy to trigger the glucosinolates content was carried out with water (control), methyl jasmonate (250 µM), jasmonic acid (250 µM) and DL-methionine (10mM) before the elicitor exogenous treatment. The results showed that almost all treatments could enhance effectively the total glucosinolates content in the sprouts, achieving the most significant increases from 34 to 100% of increase in broccoli and from 45 to 118% of increase in radish sprouts after MeJA priming and treatments. Consequently, our work demonstrates the feasibility of using elicitors, such as plant stress hormones, by priming and exogenously, as a way of increase the phytochemical profile of these sprouts to enhance their consumption in the diet.
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    Systematic review on the metabolic interest of glucosinolates and their bioactive derivatives for human health
    (MDPI, 2023-03-15) Núñez Gómez, Vanesa; Costa-Pérez, Antonio; Baenas Navarro, Nieves; Di Pede, Giuseppe; Achour, Mariem; Manach, Claudine; Mena, Pedro; Del Rio, Daniele; García-Viguera, Cristina; Moreno, Diego A.; Domínguez-Perles, Raúl; Tecnología de Alimentos, Nutrición y Bromatología; Facultad de Veterinaria
    In the last decade, most of the evidence on the clinical benefits of including cruciferous foods in the diet has been focused on the content of glucosinolates (GSL) and their corresponding isothiocyanates (ITC), and mercapturic acid pathway metabolites, based on their capacity to modulate clinical, biochemical, and molecular parameters. The present systematic review summarizes findings of human studies regarding the metabolism and bioavailability of GSL and ITC, providing a comprehensive analysis that will help guide future research studies and facilitate the consultation of the latest advances in this booming and less profusely researched area of GSL for food and health. The literature search was carried out in Scopus, PubMed and the Web of Science, under the criteria of including publications centered on human subjects and the use of Brassicaceae foods in different formulations (including extracts, beverages, and tablets), as significant sources of bioactive compounds, in different types of subjects, and against certain diseases. Twenty-eight human intervention studies met inclusion criteria, which were classified into three groups depending on the dietary source. This review summarizes recent studies that provided interesting contributions, but also uncovered the many potential venues for future research on the benefits of consuming cruciferous foods in our health and well-being. The research will continue to support the inclusion of GSL-rich foods and products for multiple preventive and active programs in nutrition and well-being.