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Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism

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dc.contributor.authorSánchez-Pujante, Pedro Joaquín
dc.contributor.authorGionfriddo, Matteo
dc.contributor.authorDíaz-Vivancos, Pedro
dc.contributor.authorAlmagro Romero, Lorena
dc.contributor.authorPedreño García, María Ángeles
dc.contributor.authorSabater Jara, Ana Belén
dc.contributor.departmentBiología Vegetales
dc.date.accessioned2025-07-04T06:38:52Z
dc.date.available2025-07-04T06:38:52Z
dc.date.issued2020-02-22
dc.description© 2020, Elsevier GnbH. Allrights reserved. This document is the Published version of a Published Work that appeared in final form in Journal of Plant Physiology. To access the final edited and published work see https://doi.org/10.1016/j.jplph.2020.153136
dc.description.abstractElicited broccoli suspension-cultured cells (SCC) provide a useful system for obtaining bioactive compounds, including glucosinolates (GS) and phenolic compounds (PCs). In this work, coronatine (Cor) and methyl jas monate (MJ) were used to increase the bioactive compound production in broccoli SCC. Although the use of Cor and MJ in secondary metabolite production has already been described, information concerning how elicitors affect cell metabolism is scarce. It has been suggested that Cor and MJ trigger defence reactions affecting the antioxidative metabolism. In the current study, the concentration of 0.5 μM Cor was the most effective treatment for increasing both the total antioxidant capacity (measured as ferulic acid equivalents) and glucosinolate content in broccoli SCC. The elicited broccoli SCC also showed higher polyphenol oxidase activity than the control cells. Elicitation altered the antioxidative metabolism of broccoli SCC, which displayed biochemical changes in antioxidant enzymes, a decrease in the glutathione redox state and an increase in lipid peroxidation levels. Furthermore, we studied the effect of elicitation on the protein profile and observed an induction of defence-related proteins. All of these findings suggest that elicitation not only increases bioactive compound production, but it also leads to mild oxidative stress in broccoli SCC that could be an important factor triggering the production of these compounds.
dc.formatapplication/pdfes
dc.identifier.citationJournal of Plant Physiology 248 (2020) 153136
dc.identifier.doihttps://doi.org/10.1016/j.jplph.2020.153136
dc.identifier.issnPrint: 0176-1617
dc.identifier.issnElectronic: 1618-1328
dc.identifier.urihttp://hdl.handle.net/10201/156944
dc.languageenges
dc.publisherElsevier
dc.relationFundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (No. 19876/GERM/15).es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0176161720300249?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntioxidative metabolism
dc.subjectBroccoli suspension-cultured cells
dc.subjectCoronatine
dc.subjectGlucosinolates
dc.subjectMethyl jasmonate
dc.subjectPhenolic compounds
dc.titleEnhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolismes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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relation.isAuthorOfPublication6a60e21b-276a-41c1-9f7e-16b7c933da77
relation.isAuthorOfPublication4c6e7506-0db4-477e-8704-2a1783040719
relation.isAuthorOfPublication.latestForDiscovery26b10f99-26d5-4ed0-a830-5b27d6665ae6
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