Publication: Ultrastructural and hormonal metabolic studies of rat liver maintained in vitro by perfusion at 30° C and 37O C: a time course study by TEM, SEM and RIA
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Date
1989
Authors
Hassan, Ibrahim M. ; Al-Ali, Saad Y. ; Hassan, Mithal
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Isolated rat liver perfusion system has been
extensively used for metabolic and functional studies.
Results derived from the application of this system may
reflect true biochemical changes but they may also be
associated with some structural changes. This study was
undertaken to correlate the cytological changes and
functional integrity of isolated rat liver perfused in vitro
at normal physiological temperature (37°C) and 30°C,
using a non-recirculating system. The livers were
perfused for 3 hours with modified Ham's F10 culture
medium supplemented with thyroxine hormone (T4).
The hepatocyte structural integrity was studied by light
microscopy, transmission and scanning electron microscopy.
The triiodothyronine (T3) and T4 hormones in the
perfusion medium and the effluent fractions were
assessed by radioimmunoassay. The livers perfused at
30°C remained morphologically intact at the ultrastructural
level for 3 hours whilst at 37"C, hepatocytes in the
centrilobular zone exhibited marked structural alterations.
The percentage of T4 uptake was significantly
higher (P < 0.01) in livers perfused at 30°C (50.8 * 7.7%
vs 38 * 7.7%, 37"C), but the net T3 output (3.16 * 1.04
pg) and the conversion of T4 to T3 (4 * 0.62%) were
significantly higher (P < 0.001) in livers perfused at 37°C in comparison to livers perfused at 30°C (1.61 * 0.84 pg
and 1.68 2 0.76%, respectively). In conclusion, at 30°C
the hepatic T4 uptake is not inhibited, but the rate of T4
to T3 conversion has decreased, additionally the livers
remain morphologically well preserved throughout the
experimental period. At 37"C, although T4 to T3 conversion
is higher, structurally the livers could not be maintained
intact for more than 2 hours. Therefore, isolated
rat livers perfused in vitro at 30°C offer the best compromise
for further morphological and metabolic studies.
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