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Pluripotent stem cells isolated from umbilical cord form embryonic like bodies in a mesenchymal layer culture

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dc.contributor.authorTsagias, Nikos
dc.contributor.authorKouzi-Koliakos, Kokkona
dc.contributor.authorKaragianis, Vasileios
dc.contributor.authorTsikouras, P.
dc.contributor.authorKoliakos, George G.
dc.date.accessioned2020-03-18T13:20:31Z
dc.date.available2020-03-18T13:20:31Z
dc.date.issued2015
dc.description.abstractRecently the matrix of umbilical cord began to use as an alternative source of stem cells additionally to the blood of umbilical cord. Umbilical cord has been used mainly for mesenchymal stem cell banking. The immunological characteristics of mesenchymal stem cells in combination with their ability to avoid rejection make them an attractive biological material for transplantations. In this study the isolation of small in size pluripotent stem cells from umbilical cord expressing early transcription factors with characteristics that resemble to embryonic stem cells is investigated. Pluripotent stem cells were isolated from human umbilical cords, by a new strategy method based on unique characteristics such as the small size and the positivity on early transcription factors OCT and Nanog. An enriched population of CXCR4+ OCT+ Nanog+ CD45- small stem cells from the cord was isolated. This fraction was able to create alkaline phosphatase positive like spheres forms in a mesenchymal layer with multilineage differentiation capacity. Our results were assessed by RT PCR and electophoresis for the pluripotent genes. These data suggest that umbilical cord provides an attractive source not only of mesenchymal stem cells but moreover of pluripotent stem cells. The method described herein should be applied in the field of stem cell banking in addition to the classical umbilical cord harvesting method. Isolation of a population of cells with pluripotent characteristics from umbilical cord. Adoption of a second centrifugation step for the pluripotent stem isolation. Increasing the value of the cord and explaining the pluripotency. This work will enhance the value of umbilical cord harvesting.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology, Vol. 30, n.º 3 (2015)
dc.identifier.doihttps://doi.org/10.14670/HH-30.373
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/88214
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectUmbilical cordes
dc.subjectPluripotent stem cellses
dc.subjectDifferentiationes
dc.subjectTransplantationes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titlePluripotent stem cells isolated from umbilical cord form embryonic like bodies in a mesenchymal layer culturees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nº3 (2015)