Publication: The tumor necrosis factor a of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner
Authors
García-Castillo, Jesús ; Chaves-Pozo, Elena ; Olivares, Pedro ; Pelegrin, Pablo ; Meseguer, José ; Mulero Méndez, Victoriano Francisco
item.page.secondaryauthor
item.page.director
Publisher
Springer
publication.page.editor
publication.page.department
DOI
10.1007/s00018-004-4068-1
item.page.type
info:eu-repo/semantics/article
Description
©2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Published version of a Published Work that appeared in final form in Cellular and Molecular Life Sciences. To access the final edited and published work see https://doi.org/10.1007/s00018-004-4068-1
Abstract
Information on the bioactivities of non-mammalian cytokines is scant due to the lack of the recombinant molecules and specific antibodies. We produced the mature predicted peptide of tumor necrosis factor a (TNFa) from the bony fish gilthead seabream (Sparus aurata L.) (sbTNFa), and its biological role was determined in vitro and in vivo. We first demonstrated by analytical size-exclusion chromatography that sbTNFa is an oligomeric protein but the dimer appears to predominate over the trimeric form, in contrast to mammalian TNFa. Intraperitoneal injection of native sbTNFa resulted in (i) priming of the respiratory burst of the peritoneal exudate and head-kidney (HK) leukocytes, the latter being the bone marrow equivalent in fish; (ii) rapid recruitment of phagocytic granulocytes to the injection site, and (iii) induction of granulopoiesis in the HK. Interestingly, sbTNFa was able to induce a strong proliferation of HK cells in vitro, whereas human TNFa did not. Conversely, sbTNFa was not cytotoxic for murine L929 fibroblasts.
publication.page.subject
Citation
Cellular and Molecular Life Sciences, volumen 61, nº 11, año 2004, páginas 1331-1340.
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/